New Mn(II), Ni(II), Co(II) and Cu(II) complexes of an azo dye ligand based on p‐phenylenediamine with 5‐nitro‐8‐hydroxyquinoline were synthesized and characterized using elemental analysis, inductive ...coupled plasma analysis, molar conductance, powder X‐ray diffraction, thermogravimetric analysis, magnetic moment measurements, and infrared, 1H NMR, electron ionization mass and UV–visible spectral studies. The spectral and analytical data reveal that the azo dye ligand acts as a monobasic bidentate ligand via deprotonated OH and nitrogen atom of the quinoline ring. The data support the formulation of all complexes with a 2:1 ligand‐to‐metal ratio, except the Mn(II) complex that has a mononuclear formula. All complexes have an octahedral structure. The molar conductance data reveal that all the metal complexes are non‐electrolytic in nature. From the X‐ray data, the average particle size of the ligand and its complexes is 0.32–0.64 nm. The colour fastness to light, washing, perspiration, sublimation and rubbing of the prepared ligand and its complexes on polyester fabrics and colorimetric properties were measured. The results reveal that the ligand and its complexes have a good to moderate affinity to polyester fibres.
New Mn(II), Ni(II), Co(II) and Cu(II) complexes of an azo dye ligand were synthesized. Spectral and analytical data revealed that the azo dye ligand acts as a monobasic bidentate ligand. X‐ray data for the investigated compounds were obtained. The colour fastness of the prepared ligand and its complexes on polyester fabrics and colorimetric properties were measured.
Background
An association exists between hepatitis C virus (HCV) infection and non-Hodgkin’s lymphoma (NHL), but a causal relationship is not fully established. HCV is a lymphotropic virus that ...represents a major etiologic agent of mixed cryoglobulinemia (MC) type II which is characterized by a low-grade B cell clonal lymphoproliferative disorder that usually progresses to a more aggressive malignant lymphoma. This study assessed the role of cryoglobulin and B lymphocyte stimulator (BLys) in the pathogenesis of NHL in chronic HCV patients.
Methods
Sixty HCV patients, 30 free of B cell NHL (group I) and 30 with B cell NHL (group II), and 30 healthy controls (group III) were studied. Qualitative cryoglobulin assessment and a quantitative assay for BLys were done.
Results
In group II, BLyS positivity rate was 1.5-fold higher than of group I (
p
≤ 0.01). A positive association was found between positivity rate of MC and the level of BLyS (
p
≤ 0.01).
Conclusion
High BLyS levels were associated with HCV-associated lymphoproliferative disorder coupled with positive MC.
Seven Cu(II)-complexes with 2-amino-5-substituted aryl-1,3,4-oxadiazole Schiff bases are presented. The donors and possible geometries of the complexes were investigated by elemental and thermal ...(differential thermal analysis and thermogravimetric analysis) analyses, molar conductance, magnetic moments, IR,
1
H-NMR, ESR, UV-Vis, and mass spectra. The ligands are bidentate, coordinating through the nitrogen of azomethine and the nearest nitrogen to it. The results are supported by 3-D molecular modeling of 2 using CS Chem 3-D Ultra Molecular Modeling and Analysis Program. The investigated complexes have been screened for in-vitro antibacterial (Escherichia coli and Staphylococcus aureus) and antifungal (Aspergillus flavus and Candida albicans) activities. The qualitative and quantitative antimicrobial activities prove that the complexes are very active against the tested microorganisms.
A novel azo dye ligand formed by the coupling of L‐histidine with 2‐hydroxy‐1‐naphthaldyhide(H2L) and its Ru3+, Pd2+ and Ni2+ nano‐sized complexes were obtained and described by elemental analysis, ...TGA, magnetic moment measurements, molar conductance, UV‐Vis, ESR, X‐ray powder diffraction, IR, SEM, TEM, 1H‐nmr, 13C‐nmr, and EI‐mass spectral studies. The analytical results and spectral studies detected that the H2L ligand acts as dibasic tetradentate via aldehyde oxygen, azo nitrogen and deprotonated OH and COOH groups. The data showed the paramagnetic Ru3+ complex has octahedral geometry while Pd2+ and Ni2+ have square planar structures. The molar conductance measurements display all complexes are nonelectrolyte. The crystallinity, morphology and average particle size data revealed the prepared complexes were formed in the Nano scale. The average particle size as calculated from TEM images are found to be 13.72, 64.52 and 115.00 nm for Ru3+, Pd2+ and Ni2+ chelates, respectively. The catalytic activities of these compounds were checked for oxidation of 2‐amino phenol to 2‐amino‐3H phenoxazine‐3‐one as heterogeneous catalysts. A 96, 31 and 21% catalytic conversion are found when using Ru(III), Pd(II) and Ni(II) complexes respectively.
Ru3+, Pd2+ and Ni2+ nano‐sized complexes of L‐histidine azo dye ligand were obtained. The catalytic activities of these compounds were checked for oxidation of 2‐amino phenol to 2‐amino‐3H phenoxazine‐3‐one as heterogeneous catalysts. A 96, 31 and 21% catalytic conversion are found when using Ru(III), Pd(II) and Ni(II) complexes respectively.
Cu(II), Co(II), Mn(II), Cr(III) and Mn(III) chelates of the ligand obtained by the condensation of 4H‐3,5‐diamino‐1,2,4‐triazole and 2‐hydroxy‐1‐naphthaldehyde have been isolated and characterized by ...means of elemental analysis, inductively coupled plasma analysis, mass spectrometry, Fourier transform infrared and UV–visible spectroscopies, magnetic measurements and thermal analysis. The analytical results indicated the formation of the metal complexes in a ligand‐to‐metal molar ratio of 1:2. The ligand is connected to the metal ion in a tetradentate fashion coordinating to the metal center through the azomethine nitrogens and deprotonated phenolic OH groups. The spectral and magnetic results supported the formation of octahedral geometry around the Cu(II), Mn(III) and Cr(III) centers while Co(II) and Mn(II) centers were confirmed to have tetrahedral geometry. Molecular docking was used to predict the binding between the ligand and the oncogenic protein H‐ras (121p). The antiproliferative activity of the compounds under study was evaluated in vitro using MTT assay against liver carcinoma (HepG2) and breast cancer (MCF‐7) cell lines comparing with cisplatin. The binding mode of selected metal chelates with salmon serum DNA was investigated using the electronic absorption titration method.
Cu(II), Co(II), Mn(II), Cr(III) and Mn(III) complexes of a Schiff base ligand have been synthesized and characterized. Molecular docking and binding energy calculations of the ligand with H‐ras (121p) indicated that the ligand is an efficient inhibitor of liver and breast cancer. The antitumor activities of the ligand and its complexes were evaluated against the two cancer cell lines HepG2 and MCF‐7 and compared with that of the well‐known anticancer drug cisplatin.
A new set of Ag(I), In (III) and Sn (II) chelates based on azo dye ligand of 5‐aminosalicylic acid (H2L) were prepared. The structures of this novel chelates have already been confirmed by elemental ...analysis, thermo‐gravimetric analysis, molar conductance, X‐ray diffraction (XRD), UV–Vis, Fourier‐transform infrared spectroscopy (FT‐IR), and mass spectra. The lower conductivity values showed the non‐electrolytic nature. The kinetic and thermodynamic parameters were determined through using Coats–Redfern model, also, potential application; the antibacterial activity of the target compounds was tested by examining their effect on the development of various strains of bacteria. Utilizing UV–visible absorption measurements, we investigated the interaction between calf thymus DNA and the evaluated compounds. According to the findings, the intercalative binding approach, the chelates interact with DNA by assessing the binding constants. Additionally, the in vitro antimicrobial properties of the ligand and its metal chelates were evaluated. The theoretical density functional theory (DFT) simulations for the azo dye ligand (H2L) were completed by using B3LYP/6‐311G++ (d,p) basis functions, and the B3LYP/LanL2DZ for Ag(I), In (III), and Sn (II) chelatesshows that the uninuclear Ag(I) and Sn(II) chelates under consideration have a tetrahedral geometry. Although the experimental data are in agreement with the octahedral shape for binuclear In (III) chelate. Molecular docking simulations were carried out using molecular operating environment (MOE) software for the ligand and its chelates. Docking simulation results indicated that the Ag(I) chelate was more potent and exhibited a higher affinity for the (3QKK) protein than the In (III) and Sn (II) chelates. This is in agreements with the biological evaluations under studies.
Geometry optimized structures and HOMO‐LUMO band gap of precursors, ligand along with Ag(I), In (III), and Sn (II) chelates.
A novel Schiff base namely (E)‐3‐((2,6‐dihydroxypyrimidin‐4‐ylimino)methyl)‐4H‐chromen‐4‐one and its Co (II), N (II)i, Cu (II) and Cd (II) complexes have been synthesized and proved by elemental ...analysis, molar conductance, thermal analysis (TGA), Inductive Coupled plasma (ICP), magnetic moment measurements, X‐ray powder diffraction, IR, EI‐mass,1H NMR, 13C NMR,UV–Vis. and ESR spectral studies. On the basis of these data, it is evident that the Schiff base acts as bidentate via oxygen atom of carbonyl group and azomethine nitrogen atom for Co (II) complex; monobasic bidentate ligand for Ni (II), Cu (II) and Cd (II) complexes via oxygen atom of hydroxyl group and nitrogen atom of pyrimidine ring. The results showed all complexes have octahedral geometry. The average particle size of the ligand and its complexes were found to be 1.010–0.343 nm. The pharmacological action (antioxidant, antimicrobial and anticancer) of the prepared compounds is studied. The antitumor activity of the ligand and its metal complexes is evaluated against human liver carcinoma (HEPG2) cell. The data displayed the Co (II) complexes strong cytotoxicity where IC50 values of Co (II) complex and 5‐fluorouracil (stander drug) are 9.33 and 7.86 μg/ml respectively. The Co (II) and Cd (II) complexes have antibacterial activity more than ampicillin (stander drug). The interaction of the synthesized compounds with calf‐thymus DNA (CT‐DNA) has been performed via absorption spectra and viscosity technique. The DNA‐ binding constants have been determined.
A novel Schiff base namely (E)‐3‐((2,6‐dihydroxypyrimidin‐4‐ylimino)methyl)‐4H‐chromen‐4‐one and its Co(II), N(II)i, Cu(II) and Cd(II) complexes have been synthesized and proved. The pharmacological action (antioxidant, antimicrobial and anticancer)of the prepared compounds were studied.
A new series of UO2(II) and ZrO(II) azo‐complexes based on 5‐nitro‐8‐hydroxyquinoline; UO2(H2L1)(NO3)EtOH (1), ZrO(H2L1)(NO3)H2O (2), UO2(HL2)(NO3)EtOH3H2O (3), ZrO(HL2)(NO3)EtOH (4), ...UO2(HL3)(NO3)(H2O)32H2O (5) and ZrO(HL3)(NO3)EtOH (6); have been synthesized. The structure of these complexes has been characterized using elemental analysis, thermal analysis, molar conductance, UV–vis, IR, electron impact mass, X‐ray powder diffraction and NMR spectra. The results revealed the formation of non‐electrolyte mononuclear complexes via the N atom of the azo group or of the quinoline ring and the oxygen atom of the deprotonated OH. Fluorescence properties of the synthesized complexes have been examined and the fluorescence quantum yield (Φf) has been determined. The complexes have been tested as cell staining and imaging under the fluorescent microscope. The data showed that complexes 1 and 2 efficiently stain the nuclei in addition to some focal cytoplasmic areas. Other than complexes 3 and 4 exclusively stained the nuclei. On the other hand, complexes 5 and 6 stained the cytoplasm exclusively. It has been demonstrated that complex 4 was the most effective in cell staining. The binding constant (Kb) with DNA was calculated using UV–vis absorption titration and fluorescence spectral methods. It was concluded that complex 4 can be used effectively as fluorescent probes in studying cell biology.
Highlights
New fluorescent UO2(II) and ZrO(II) azo‐complexes have been synthesized and characterized.
The complexes were tested as cell staining and imaging under fluorescent microscope.
The binding constant (Kb) with DNA has been calculated using UV‐vis absorption titration and fluorescence spectral methods.
New ZrO(II) azo‐complexe based on 5‐nitro‐8‐hydroxyquinoline can be effectively used as fluorescent probes in studying cell biology.
