Cell migration and metastasis greatly contribute to the progression of tumors. Secreted Protein and Rich in Cysteine （SPARC）, as a multi-faceted protein, is highly expressed in highly metastatic ...tumors while low or undetectable in less metastatic types with aberrant promoter methylation. In highly metastatic tumors, such as glioblastomas, melanoma, breast cancer and prostate cancer, SPARC promotes bone metastasis and epithelial-mesenchymal transition (EMT). In contrast, this protein acts as an anti-tumor factor in anti-angiogenesis, pro-apoptosis, cell proliferation inhibition and cell cycle arrest in less metastatic tumors, such as neuroblastoma, ovarian cancer, pancreatic cancer, colorectal cancer and gastric cancer. Here, we summarize and analyze the paradoxical role of SPARC in different tumors. We believe that further studies on truncated, alternative splicing variants and signal peptide of SPARC are required to elucidate the distinct effects. Most notably, SPARC variants probably play a crucial role in regulation of transforming growth factor beta (TGF-β) induced EMT. This review also provides strategies to target or use SPARC (full-length, truncated and splicing variants) for therapeutic purposes.
•Hydrochemistry of three different types of groundwater in different seasons was characterized.•Groundwater quality: fissure groundwater > karst groundwater > pore groundwater.•Anthropogenic inputs ...increase groundwater nitrate concentrations.•Health risk of nitrate in groundwater is higher in children than in adults.
Groundwater resources fulfill a key role in socioeconomic development and residential water use in the Dawen River Basin (DRB). This study sampled and tested three types of groundwater in the DRB, including pore groundwater (PGW), fissure groundwater (FGW), and karst groundwater (KGW). Groundwater in the DRB was assessed for hydrochemical characteristics, quality, and hazards to human health by nitrate contents. Results identified the dominant groundwater anion and cation to be HCO3− and Ca2+, respectively, whereas groundwater was generally weakly alkaline hard-fresh water. The groundwater contents of main chemical components during wet season exceeded those in the dry season, with the rank of groundwater types by chemical component contents being: PGW > KGW > FGW. A Durov diagram indicated groundwater in the DRB to be mixed HCO3-Ca and HCO3·SO4–Ca·Mg types. Rock weathering (silicate and carbonate rocks) was found to be the predominant process regulating groundwater chemistry of the DRB, whereas human activities also had a significant impact, particularly for high NO3− related to agricultural activities. Groundwater quality of the DRB was relatively good overall, falling into the good and moderate classification grades, with that of the PGW the worst. Long-term human direct consumption or exposure to groundwater of DRB poses certain health risks due to groundwater nitrate, with the risk for children highest. The spatial variations in quality of groundwater and nitrate health hazard in the DRB were relatively consistent, with better quality and lower health risk of PGW and FGW in the east than in the west, and that of KGW better in the center than in the east and west. The present study can provide some reference for groundwater safety and protection management in DRB and other similar areas in the world.
Alginate-based nanocarriers are propitious vehicles used for the delivery of bioactive compounds (bioactives). In this area, calcium alginate and sodium alginate are the most promising wall materials ...because they are nontoxic, comparatively cheap, simple in production, biocompatible and biodegradable. In this review, we have highlighted different alginate-based nanocarriers such as nanoparticles, nanofibers, nanoemulsions, nanocomplexes, and nanohydrogels; also entrapment of different bioactives within alginate nanocarriers and their bioavailability in the gastric environment has been comprehensively discussed. Being biopolymers, alginates can be exploited as emulsifiers/ encapsulants for entrapment and delivery of different bioactives such as vitamins, minerals, essential fatty acids, peptides, essential oils, bioactive oils, polyphenols and carotenoids. Furthermore, the use of alginate-based nanocarriers in combination with other polysaccharides/ emulsifiers was recognized as the most effective and favorable approach for the protection, delivery and sustained release of bioactives.
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•Different properties of alginate make it an ideal carrier for bioactive delivery systems.•Alginate-based nanocarriers (ABNs) improve techno-functional activities of loaded bioactives.•ABNs include nanoparticles, nanocomplexes, nanoemulsions, nanohydrogels and nanofibers.•ABNs have been applied for various bioactives, e.g., phenolics, carotenoids, essential oils, etc.
The advancement in high-throughput sequencing analysis and the evaluation of chromatin state maps have revealed that eukaryotic cells produce many non-coding transcripts/RNAs. Further, a strong ...association was observed between some non-coding RNAs and cancer development. The mitogen-activated protein kinases (MAPK) belong to the serine–threonine kinase family and are the primary signaling pathways involved in cell proliferation from the cell surface to the nucleus. They play an important role in various human diseases. A few non-coding RNAs associated with the MAPK signaling pathway play a significant role in the development of several malignancies, including liver cancer. In this review, we summarize the molecular mechanisms and interactions of microRNA, lncRNA, and other non-coding RNAs in the development of liver cancer that are associated with the MAPK signaling pathway. Further, we briefly discuss the therapeutic strategies for liver cancer related to ncRNA and the MAPK signaling pathway.
Diabetic nephropathy is one of the most serious microvascular complications of diabetes mellitus, with increasing prevalence and mortality. Currently, renal function is assessed clinically using ...albumin excretion rate and glomerular filtration rate. But before the appearance of micro-albumin, the glomerular structure has been severely damaged. Glomerular filtration rate based on serum creatinine is a certain underestimate of renal status. Early diagnosis of diabetic nephropathy has an important role in improving kidney function and delaying disease progression with drugs. There is an urgent need for biomarkers that can characterize the structural changes associated with the kidney. In this review, we focus on the early glomerular and tubular structural alterations, with a detailed description of the glomerular injury markers SMAD1 and Podocalyxin, and the tubular injury markers NGAL, Netrin-1, and L-FABP in the context of diabetic nephropathy. We have summarized the currently studied protein markers and performed bioprocess analysis. Also, a brief review of proteomic and scRNA-seq method in the search of diabetic nephropathy.
To investigate the effects of ketamine on glucose uptake and glucose transporter (GLUT) expression in depressive-like mice. After HA1800 cells were treated with ketamine, ...2-N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino-2-Deoxyglucose (2-NBDG) was added to the cells to test the effects of ketamine on glucose uptake, production of lactate, and expression levels of GLUT, ERK1/2, AKT, and AMPK. Adult female C57BL/6 mice were subjected to chronic unpredictable mild stress (CUMS), 27 CUMS mice were randomly divided into the depression, ketamine (i.p.10 mg/kg), and FR180204 (ERK1/2 inhibitor, i.p.100 mg/kg) + ketamine group. Three mice randomly selected from each group were injected with
F-FDG at 6 h after treatment. The brain tissue was collected at 6 h after treatment for p-ERK1/2 and GLUTs. Treatment with ketamine significantly increased glucose uptake, extracellular lactic-acid content, expression levels of GLUT3 and p-ERK in astrocytes and glucose uptake in the prefrontal cortex (P < 0.05), and the immobility time was significantly shortened in depressive-like mice (P < 0.01). An ERK1/2 inhibitor significantly inhibited ketamine-induced increases in the glucose uptake in depressive-like mice (P < 0.05), as well as prolonged the immobility time (P < 0.01). The expression levels of p-ERK1/2 and GLUT3 in depressive-like mice were significantly lower than those in normal control mice (P < 0.01). Ketamine treatment in depressive-like mice significantly increased the expression levels of p-ERK1/2 and GLUT3 in the prefrontal cortex (P < 0.01), whereas an ERK1/2 inhibitor significantly inhibited ketamine-induced increases (P < 0.01).Our present findings demonstrate that ketamine mitigated depressive-like behaviors in female mice by activating the ERK/GLUT3 signal pathway, which further increased glucose uptake in the prefrontal cortex.
Abnormal RNA metabolism, regulated by various RNA binding proteins, can have functional consequences for multiple diseases. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is an important RNA ...binding protein, that regulates various RNA metabolic processes, including transcription, alternative splicing of pre-mRNA, translation, miRNA processing and mRNA stability. As a potent splicing factor, hnRNP A1 can regulate multiple splicing events, including itself, collaborating with other cooperative or antagonistical splicing factors by binding to splicing sites and regulatory elements in exons or introns. hnRNP A1 can modulate gene transcription by directly interacting with promoters or indirectly impacting Pol II activities. Moreover, by interacting with the internal ribosome entry site (IRES) or 3′-UTR of mRNAs, hnRNP A1 can affect mRNA translation. hnRNP A1 can alter the stability of mRNAs by binding to specific locations of 3′-UTR, miRNAs biogenesis and Nonsense-mediated mRNA decay (NMD) pathway. In this review, we conclude the selective sites where hnRNP A1 binds to RNA and DNA, and the co-regulatory factors that interact with hnRNP A1. Given the dysregulation of hnRNP A1 in diverse diseases, especially in cancers and neurodegeneration diseases, targeting hnRNP A1 for therapeutic treatment is extremely promising. Therefore, this review also provides the small-molecule drugs, biomedicines and novel strategies targeting hnRNP A1 for therapeutic purposes.
An aqueous nanosuspension of agrochemicals unlike pharmaceutics has to achieve massive production in an effective way, capable to ensure sufficient profits in commercialization. This work implements ...the flash nanoprecipitation (FNP) technique to effectively generate agrochemical nanosuspension, anticipatedly overcoming such an obstacle. Azoxystrobin, a broad spectrum fungicide, in either acetone or ethanol is used herein as a mode agrochemical. To ensure a green and practical utilization, three kinds of commercially available and eco-friendly surfactants, i.e., poly(ethylene glycol)-
block
-poly(lactic-
co
-glycolic acid) (PEG-
b
-PLGA), Tween 80 and alkyl polyglucosides (APGs), are employed for stabilizing the nanoparticles. The results show that the polymeric stabilizer, PEG-b-PLGA, has the best stabilization efficiency, and can maintain the particles below 100 nm for at least three weeks. The azoxystrobin load of the nanoparticles reaches as high as 77 wt.%, beneficial to enhancing the biological potency. Moreover, the FNP brings the particles a much smaller size, narrower size distribution, better size stability, and higher biological efficacy than the ones made via a traditional method of the drop and stir (DS). The nanosuspensions present superior fungicidal performances over a prevailing counterpart from Syngenta. This study proves an enhanced biological potency and reduced dosage of agrochemical nanosuspension made via the FNP, indicating a remarkable advantage of the FNP over the conventional preparation. The integration of a super-high load, eco-friendly excipients, intensified preparation process, enhanced potency, and reduced dosage creates a promising pathway to generate a green aqueous nanosuspension of agrochemicals.
Diabetes mellitus (DM) is a prevalent chronic disease in the 21st century due to increased lifespan and unhealthy lifestyle choices. Extensive research indicates that exercise can play a significant ...role in regulating systemic metabolism by improving energy metabolism and mitigating various metabolic disorders, including DM. Irisin, a well-known exerkine, was initially reported to enhance energy expenditure by indicating the browning of white adipose tissue (WAT) through peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) signaling. In this review, we summarize the potential mechanisms underlying the beneficial effects of Irisin on glucose dysmetabolism, including reducing gluconeogenesis, enhancing insulin energy expenditure, and promoting glycogenesis. Additionally, we highlight Irisin’s potential to improve diabetic vascular diseases by stimulating nitric oxide (NO) production, reducing oxidative and nitrosative stress, curbing inflammation, and attenuating endothelial cell aging. Furthermore, we discuss the potential of Irisin to improve diabetic cardiomyopathy by preventing cardiomyocyte loss and reducing myocardial hypertrophy and fibrosis. Given Irisin’s promising functions in managing diabetic cardiomyopathy and vascular diseases, targeting Irisin for therapeutic purposes could be a fruitful avenue for future research and clinical interventions.
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•Irisin can reduce gluconeogenesis, promote energy expenditure and glycogenesis.•Endothelial dysfunction might be the main mechanism of Diabetic Vascular Diseases, irisin can relieve it.•Irisin might prevent cardiomyocyte loss by regulating kinds of cell death.•The powerful anti-inflammatory effects of Irisin can ameliorate atherosclerosis.
Competing endogenous RNAs (ceRNAs) containing microRNA response elements can competitively interact with microRNA via miRNA response elements, which can combine non-coding RNAs with protein-coding ...RNAs through complex ceRNA networks. CeRNAs include non-coding RNAs (long non-coding RNAs, circular RNAs, and transcribed pseudogenes) and protein-coding RNAs (mRNAs). Molecular interactions in ceRNA networks can coordinate many biological processes; however, they may also lead to ceRNA network imbalance and thus contribute to cancer occurrence when disturbed. Recent studies indicate that many dysregulated RNAs derived from lung cancer may function as ceRNAs to regulate multitudinous biological functions for lung cancer, including tumor cell proliferation, apoptosis, growth, invasion, migration, and metastasis. This study therefore reviewed the research progress in the field of non-coding and protein-coding RNAs as ceRNAs in lung cancer, and highlighted validated ceRNAs involved in biological lung cancer functions. Furthermore, the roles of ceRNAs as novel prognostic and diagnostic biomarkers were also discussed. Interpreting the involvement of ceRNAs networks in lung cancer will provide new insight into cancer pathogenesis and treatment strategies.
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