Different strains of Newcastle disease viruses (NDV) or Sendai viruses (SV) are used to induce the production of human leukocyte multi subtype interferon-alpha (HuIFN-αN3). Their inducing capacity ...can be enhanced in different ways. One includes 10% PBS washout of Holocene minerals (HM). The presented study aims to compare the HuIFN-αN3 inducing capacity of NDV ZG1999HDS or SV (Cantell strain) strain in vitro, and to evaluate the enhancing effect of 10% PBS washouts of HM on both viruses. The NDV strains' ZG1999HDS interferon inducing capacity (483.23 ± 4.5 pg/mL) was similar to that of the SV (Cantell strain) (584.16 ± 5.9 pg/mL). It was shown that the HuIFN-αN3 inducing capacity of the strain of NDV ZG1999HDS can be strongly enhanced with 10% PBS washout of HM to 3818.21 ± 41.9 pg/mL and 4790.34 ± 33.5 pg/mL with SV (Cantell strain), u. The RP-HPLC analyses of such HuIFN-αN3 induced with the strain of NDV ZG1999HDS show the difference to SV (Cantell strain) induced HuIFN-αN3 in the absence of subtype α14 and the lower level of the subtype α1. The possible ways of such enhancement were also studied and it was postulated that the Fe
ions from 10% PBS washouts of HM, while stimulating the reactive oxygen species (ROS) and nitric oxide (NO) formation, activate the transcription factor NF- κB and consequently the production of HuIFN-αN3.
Among royal jelly's (RJ) various biological activities, its possible antitumour activity deserves particular attention. The purpose of this study was to investigate the influence of RJ, its bioactive ...component 10-hydroxy-2-decenoic acid (10- HDA), and human interferon-alpha (HuIFN-αN3) on the proliferation of human colorectal adenocarcinoma cells (CaCo- 2), and ascertain their effect on intracellular glutathione (GSH) level and lipid peroxidation. We studied the antiproliferative (AP) activity of RJ (0.1 g/10 mL phosphate buffer saline (PBS), HuIFN-αN3 (1000 I.U. mL⁻¹), 10-HDA at 100.0 μmol L⁻¹, and their different combinations, in the ratio 1:1, 1:2, and 2:1 on CaCo-2 cells. The GSH level was measured by glutathione assay. The lipid peroxidation was measured by malondialdehyde (MDA) assay. Single RJ had a low AP activity: 2.0 (0.5 mg mL⁻¹). HuIFN-αN3 had an AP activity of 2.5 (208.33 I.U. mL⁻¹), while 10-HDA had an AP activity of 1.5 (37.5 μmol mL⁻¹). The highest AP activity of 3.8 was obtained when RJ and HuIFN-αN3 were applied at the ratio 2:1. In that combination the level of GSH was 24.9±2.4 nmol g⁻³ of proteins (vs. 70.2±3.2 nmol g⁻³ in the control) and the level of MDA was 72.3±3.1 nmol g⁻³ (vs. 23.6±9.1 nmol g⁻³ in the control). It is generally assumed that 10-HDA, an important constituent of RJ, together with HuIFN-αN3, is responsible for the inhibition of CaCo-2 cells proliferation in vitro. In our study, however, RJ and HuIFN-αN3 applied at 2:1 decreased the level of GSH the most and significantly increased lipid peroxidation via MDA in CaCo-2 cells. Future studies should show whether these GSH- and MDA-related activities of RJ, HuIFN-αN3, 10-HDA, and their combinations may decrease the tumorigenicity index and tumorigenic potential of various tumour cells in vitro.
Among royal jelly’s (RJ) various biological activities, its possible antitumour activity deserves particular attention. The purpose of this study was to investigate the influence of RJ, its bioactive ...component 10-hydroxy-2-decenoic acid (10- HDA), and human interferon-alpha (HuIFN-αN3) on the proliferation of human colorectal adenocarcinoma cells (CaCo- 2), and ascertain their effect on intracellular glutathione (GSH) level and lipid peroxidation. We studied the antiproliferative (AP) activity of RJ (0.1 g/10 mL phosphate buffer saline (PBS), HuIFN-αN3 (1000 I.U. mL-1), 10-HDA at 100.0 μmol L-1, and their different combinations, in the ratio 1:1, 1:2, and 2:1 on CaCo-2 cells. The GSH level was measured by glutathione assay. The lipid peroxidation was measured by malondialdehyde (MDA) assay. Single RJ had a low AP activity: 2.0 (0.5 mg mL-1). HuIFN-αN3 had an AP activity of 2.5 (208.33 I.U. mL-1), while 10-HDA had an AP activity of 1.5 (37.5 μmol mL-1). The highest AP activity of 3.8 was obtained when RJ and HuIFN-αN3 were applied at the ratio 2:1. In that combination the level of GSH was 24.9±2.4 nmol g-3 of proteins (vs. 70.2±3.2 nmol g-3 in the control) and the level of MDA was 72.3±3.1 nmol g-3 (vs. 23.6±9.1 nmol g-3 in the control). It is generally assumed that 10-HDA, an important constituent of RJ, together with HuIFN-αN3, is responsible for the inhibition of CaCo-2 cells proliferation in vitro. In our study, however, RJ and HuIFN-αN3 applied at 2:1 decreased the level of GSH the most and significantly increased lipid peroxidation via MDA in CaCo-2 cells. Future studies should show whether these GSH- and MDA-related activities of RJ, HuIFN-αN3, 10-HDA, and their combinations may decrease the tumorigenicity index and tumorigenic potential of various tumour cells in vitro.
Kot del biološke aktivnosti MM (Matičnega mlečka) so avtorji preučevali njegovo protitumorsko delovanje kot tudi možno interakcijo s humanim interferonom alfa (HuIFN-αN3). Cilj opravljenih poskusov je bil preučiti vpliv kombinacije med MM in HuIFN-αN3 na proliferacijo celic Humanega kolorektalnega adenokarcinoma (CaCo-2) in njun vpliv na znotrajcelični nivo glutationa (GSH) in peroksidacijo lipidov. Avtorji so preučevali AP (Antiproliferativno) delovanje MM (0.1 g/10 mL fosfatnega pufra) (PBS), HuIFN-αN3, (1000 I.U. mL-1), 10-hidroxy-2-decenoične kisline (10-HDA) (100.0 μmol L-1) in različne kombinacije med njimi (1:1, 1:2 in 2:1) na celice CaCo-2 in vitro. Njihov vpliv na znotrajcelični nivo GSH so merili s pomočjo komercialnega kita. Peroksidacijo lipidov so merili s pomočjo meritve vrednosti malondialdehida (MDA). MM sam kaže AP aktivnost 2.0 (0.5 mg mL-1 ). HuIFN-αN3 ima AP aktivnost 2.5 (208.33 I.U. mL-1) medtem ko ima 10-HDA AP aktivnost 1.5 (37.5 μmol mL-1). AP aktivnost kombinacije MM:HuIFN-αN3 (2:1) je bila 3.8. Pri tej kombinaciji je bil viden vpliv na nivo GSH: 24.9±2.4 nmol g-3 proteinov (70.2±3.2 nmol g-3 pri kontroli). Nivo MDA je bil 72.3±3.1 nmol g-3 pri kontroli). 10-HDA je glavna sestavina MM, ki v kombinaciji s HuIFN-αN3 deluje antiproliferativno na CaCo-2 celice. MM in HuIFN-αN3 v kombinaciji 2:1 pospešujeta peroksidacijo lipidov (MDA) in zmanjšujeta nivo glutationa (GSH). Nadaljni poskusi bodo pokazali ali z GSH- in MDA- povezane aktivnosti MM, HuIFN-αN3, 10-HDA in kombinacij med njimi, zmanjšujejo indeks tumorigenosti in s tem tumorigeni potencijal različnih tumorskih celic in vitro.
Macrophages play key role in host defense and tissue repair, and thus understanding regulation of their function is important. For instance, our previous results have shown that in chicken macrophage ...system (CoMA cell line), application of a pulse of electromagnetic fields of frequencies 0.618, 1.054, 5.229, and 100.414 kHz induces production of interferon γ-like molecules. In this study, we have shown that the electromagnetic field of 100.414 kHz is the most effective in inducing synthesis of chicken interferon γ and chicken interferon γ-like molecules in CoMA cells, especially when combined with Lens culinaris agglutinin and 10% phosphate-buffered saline washouts of different Holocene minerals. A 2-minute pulse of electromagnetic field was produced by Defender’s pulse generator. Both chicken interferon γ and chicken interferon γ-like molecules from the cell supernatant were evaluated by an antiviral assay and were also analyzed with reverse-phase high-performance liquid chromatography on Phenomenex, Aeris peptide columns. Our results show that application of a single inducing factor (Lens culinaris agglutinin, 100.414 kHz electromagnetic field, 10% phosphate buffer saline washout) or combined usage of 2 of them moderately stimulated production of chicken interferon γ-like molecules (from 1.550 to 48.028 IU/mL), whereas the combination of 10% phosphate-buffered saline washout of Koprivnica rock + Lens culinaris agglutinin + 100.414 kHz/9 V resulted in an output of 162.122 IU/mL. Hence, we may conclude that a combined use of electromagnetic field, Holocene minerals, and Lens culinaris agglutinin greatly stimulates synthesis of chicken interferon γ-like molecules in CoMA cells.
Objective: To assess the antioxidant content, antimicrobial and antioxidant activities of various propolis samples. Methods: Seven propolis samples were collected from different locations in Morocco, ...which are characterized by different plant predominant vegetations. The resin, wax and balsam of hydroalcoholic extract of propolis content were identified, and the antioxidant content was analyzed with the use of HPLC and colorimetric methods. The antioxidant activity was assessed by DPPH, ABTS.+ and ferric reducing power assays. The antimicrobial activity was assessed against bacterial species, including methicillin resistant Staphylococcus aureus and Candida albicans, and expressed as the minimal inhibitory concentration. Results: The propolis samples showed significant variations in the chemical composition and in the antioxidant or antimicrobial activities even when the samples were collected from the same location. Propolis with high resin and low wax content had high level of antioxidant compounds, and strong antioxidant and antimicrobial activities. Gram-positive bacteria, especially, methicillin resistant Staphylococcus aureus were more sensitive to all propolis samples than Gram-negative bacteria and Candida albicans. Conclusions: The chemical composition and the antioxidant and antimicrobial activities of various propolis samples are different and rely on the geographic and plant origin of propolis collection. Propolis samples with low wax and high resin content might be more suitable to be used in future preclinical or clinical investigations.
Najčešći i uobičajeni pristupi u liječenju tumorskih bolesti uključuju kirurgiju, radioterapiju, kemoterapiju, imunoterapiju, ciljanu terapiju, hormonsku terapiju, transplantaciju matičnih stanica te ...sveobuhvatno personalizirano liječenje. Izostavlja se gotovo zaboravljeno liječenje primjenom različitih mikroorganizama od kojih su najzastupljeniji virusi. Njihova je neposredna primjena, ako se radi o apatogenim virusima ili njihovim genomskim modifikacijama, sve učestalija i postigla je vrlo visok stupanj primjenjivosti. Ovaj prikaz odnosi se pretežno na mogućnosti primjene mikroorganizama, u manjoj mjeri bakterija, a uglavnom virusa, u liječenju tumorskih bolesti. Neki virusi mogu zaraziti i ubiti tumorske stanice te su poznati kao onkolitički. Neki se od njih nalaze u prirodi, ali su također i modificirani, kako bi se pojačalo njihovo onkolitičko svojstvo, djelujući isključivo na tumorske stanice bez oštećivanja zdravih. U najnovije vrijeme genetički modificirani virusi postigli su stupanj javne upotrebe stavljanjem na tržište te su od FDA-a (Food and Drug Administration) dopušteni za liječenje melanoma (T-VEC)¸ dok se u Kini modificiranim adenovirusom (H101) liječi rak glave i vrata ljudi. Posebna je vrijednost virusa u liječenju tumorskih bolesti, jer se umnažaju u tumorskoj stanici dok je ne razore, tvoreći tako kompleks tumorskog antigena što omogućuje njegovo prepoznavanje od strane imunosnog sustava. Reakcija tog sustava je lokalna ili sustavna, prepoznata danas kao imunoterapija. Uspoređeni s klasičnim oblicima liječenja tumorskih bolesti, primjena onkolitičkih virusa i imunoterapija uspješniji su način s tek blagim nuspojavama. Danas suvereno usmjeruju istraživanja upravo u tom smislu.
PDF
