The amphibian skin microbiome is important in maintaining host health, but is vulnerable to perturbation from changes in biotic and abiotic conditions. Anthropogenic habitat disturbance and emerging ...infectious diseases are both potential disrupters of the skin microbiome, in addition to being major drivers of amphibian decline globally. We investigated how host environment (hydrology, habitat disturbance), pathogen presence, and host biology (life stage) impact the skin microbiome of wild Dhofar toads (
Duttaphrynus dhufarensis
) in Oman. We detected ranavirus (but not
Batrachochytrium dendrobatidis
) across all sampling sites, constituting the first report of this pathogen in Oman, with reduced prevalence in disturbed sites. We show that skin microbiome beta diversity is driven by host life stage, water source, and habitat disturbance, but not ranavirus infection. Finally, although trends in bacterial diversity and differential abundance were evident in disturbed versus undisturbed sites, bacterial co-occurrence patterns determined through network analyses revealed high site specificity. Our results therefore provide support for amphibian skin microbiome diversity and taxa abundance being associated with habitat disturbance, with bacterial co-occurrence (and likely broader aspects of microbial community ecology) being largely site specific.
A Pyrenophora teres f. teres population in North Dakota was analyzed for virulence variation and genetic diversity using 75 monospore isolates that were collected across a 4-year period (2004 to ...2007) from two North Dakota State University agricultural experiment stations at Fargo and Langdon. Pathogenicity tests by inoculation onto 22 barley differential lines at seedling stage revealed 49 pathotypes, indicating a wide range of pathogenic diversity. Two-way analysis of variance of disease ratings revealed a significant difference in the virulence among isolates and in the resistance among barley lines, as well as in the interactions between the two. 'CI5791', 'Algerian', and 'Heartland' were three barley lines showing a high level of seedling resistance to all North Dakota isolates tested; however, many previously reported resistance genes have been overcome. Forty multilocus genotypes were identified from this set of isolates by genotyping at 13 simple-sequence repeat loci. High percentages of clonal cultures were detected in the samplings from 2005 and 2007 in Fargo and 2005 in Langdon. Using a clone-corrected sample set, the mean gene diversity (h) was estimated to be 0.58, approximately the same for both locations. The calculated Wright's F(ST) value is small (0.11) but was significantly >0, indicating a significant differentiation between the Fargo and Langdon populations. In the gametic disequilibrium test, only 3 of 78 possible pairwise comparisons over all isolates showed significant (P < 0.05) nonrandom association, suggesting a random mating mode. Our results suggest that the populations from the two locations are derived from a common source and undergo frequent recombination. This research provides important information for barley breeders regarding development and deployment of cultivars with resistance to net form net blotch in this region.
Stable flies (Stomoxys calcitrans Diptera: Muscidae L.) are blood-feeding synanthropic pests, which cause significant economic losses in livestock. Stable fly antennae contain olfactory sensilla ...responsive to host and host environment-associated odours. Field observation indicated that the abundance of stable flies increased significantly in grasslands or crop fields when cattle manure slurry was applied. Major volatile compounds emanating from manure slurry were collected and identified. Behavioural responses of stable flies to those compounds were investigated in laboratory bioassays and field-trapping studies. Results from olfactometer assays revealed that phenol, p-cresol and m-cresol were attractive to adult stable flies. When tested individually, attraction was higher with lower dosages. Stable flies were most attracted to blends of phenol and m-cresol or p-cresol. Traps with binary blend lures caught more stable flies in field trials as well.
Transcription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that are easier to design to cleave at desired sites in a genome than previous types of nucleases. We ...report here the use of TALENs to rapidly and efficiently generate mutant alleles of 15 genes in cultured somatic cells or human pluripotent stem cells, the latter for which we differentiated both the targeted lines and isogenic control lines into various metabolic cell types. We demonstrate cell-autonomous phenotypes directly linked to disease—dyslipidemia, insulin resistance, hypoglycemia, lipodystrophy, motor-neuron death, and hepatitis C infection. We found little evidence of TALEN off-target effects, but each clonal line nevertheless harbors a significant number of unique mutations. Given the speed and ease with which we were able to derive and characterize these cell lines, we anticipate TALEN-mediated genome editing of human cells becoming a mainstay for the investigation of human biology and disease.
► A system for efficient and rapid genome editing with TALENs ► Generation of isogenic human cellular models of disease ► Identification of disease-associated phenotypes in multiple human cell types ► Minimal TALEN off-target effects, but significant clone-to-clone sequence variation
Efficient TALEN genome editing of human somatic and pluripotent stem cells to model metabolic disease.
On the basis of quantum chemical modeling, a kinetic scheme of methyl methacrylate polymerization initiated by benzoyl peroxide in the presence of ferrocene was proposed. The process runs by ...mechanism, which includes the reactions of free radical polymerization, and the reactions leading to formation and operability of two type coordination active sites that are capable of converting into each other. On the basis of the proposed scheme, a kinetic model was developed. This model quantitatively described the following: the experimentally determined time dependences of the methyl methacrylate conversion, the conversion dependencies of the number‐average and weight‐average molar masses of poly(methyl methacrylate), the stereoregularity values of poly(methyl methacrylate), and the time dependencies of the methyl methacrylate conversion upon its polymerization on poly(methyl methacrylate) macroinitiators obtained in radical‐initiated polymerization in the presence of ferrocene. As a result of solving the inverse kinetic problem, the parameters of temperature dependences of the reaction rate coefficients of the proposed kinetic scheme were found.
A quantum chemical simulation of the sixfold addition of 2-cyano-
iso
-propyl radical to fullerene C
60
and the formation of a number of isomeric adducts is carried out. The thermodynamic ...characteristics of the elementary acts and the pattern of the spin density delocalization in fullerene C
60
adducts containing an odd number of addends are determined. It is shown that the addition of up to four 2-cyano-
iso
-propyl radicals to fullerene C
60
leads to the attachment of radicals to the tops of the radilene of the fullerenyl part of the adducts. The addition of the fifth 2-cyano-
iso
-propyl radical proceeds to the atoms more distant from the previously attached addends in the tetrakisadduct.
18F-3'-fluoro-3'-deoxythymidine (FLT) is a nucleoside-analog imaging agent for quantifying cellular proliferation that was first reported in 1998. It accumulates during the S-phase of the cell cycle ...through the action of cytosolic thymidine kinase, TK1. Since TK1 is primarily expressed in dividing cells, FLT uptake is essentially limited to dividing cells. Thus FLT is an effective measure of cell proliferation. FLT uptake has been shown to correlate with the more classic proliferation marker, the monoclonal antibody to Ki-67. Increased cellular proliferation is known to correlate with worse outcome in many cancers. However, the Ki-67 binding assay is performed on a sampled preparation, ex vivo, whereas FLT can be quantitatively measured in vivo using positron emission tomography (PET). FLT is an effective and quantitative marker of cell proliferation, and therefore a useful prognostic predictor in the setting of neoplastic disease. This review summarizes clinical studies from 2011 forward that used FLT-PET to assess tumor response to therapy. The paper focuses on our recommendations for a standardized clinical trial protocol and components of a report so multi center studies can be effectively conducted, and different studies can be compared. For example, since FLT is glucuronidated by the liver, and the metabolite is not transported into the cell, the plasma fraction of FLT can be significantly changed by treatment with particular drugs that deplete this enzyme, including some chemotherapy agents and pain medications. Therefore, the plasma level of metabolites should be measured to assure FLT uptake kinetics can be accurately calculated. This is important because the flux constant (KFLT) is a more accurate measure of proliferation and, by inference, a better discriminator of tumor recurrence than standardized uptake value (SUVFLT). This will allow FLT imaging to be a specific and clinically relevant prognostic predictor in the treatment of neoplastic disease.
Silicon has many attractive properties for quantum computing, and the quantum-dot architecture is appealing because of its controllability and scalability. However, the multiple valleys in the ...silicon conduction band are potentially a serious source of decoherence for spin-based quantum-dot qubits. Only when a large energy splits these valleys do we obtain well-defined and long-lived spin states appropriate for quantum computing. Here, we show that the small valley splittings observed in previous experiments on Si-SiGe heterostructures result from atomic steps at the quantum-well interface. Lateral confinement in a quantum point contact limits the electron wavefunctions to several steps, and enhances the valley splitting substantially, up to 1.5 meV. The combination of electrostatic and magnetic confinement produces a valley splitting larger than the spin splitting, which is controllable over a wide range. These results improve the outlook for realizing spin qubits with long coherence times in silicon-based devices. PUBLICATION ABSTRACT
Core Ideas
Upscaling methods compared in situ measures with soil moisture from the SMAP satellite.
The accuracy of SMAP soil moisture products in annual cropland was assessed.
The spatial ...representativeness of sparse in situ networks was determined.
In 2015, NASA launched the Soil Moisture Active Passive (SMAP) satellite. Data from this satellite are being exploited to improve forecasting of extreme weather events and delivery of disaster response. International core validation sites (CVSs) have been contributing in situ soil moisture data to validate and calibrate SMAP soil moisture products. Overall the soil moisture retrieval errors have exceeded SMAP's mission requirement (errors below 0.04 m3 m−3), with the exception of some sites of annual cropland as present at the Carman (Canada) CVS. In 2016, a SMAP validation experiment was conducted at the Canadian site in Manitoba (SMAPVEX16‐MB) in an attempt to understand the differences between the SMAP soil moisture retrievals and the permanent in situ network observations. The research presented here analyzed the performance of this network in representing soil moisture within a SMAP pixel and tested five upscaling approaches. Comparisons between the permanent network and SMAPVEX16‐MB measurements (from temporary stations and field measures) confirmed agreement among these three sources of soil moisture measures. The SMAP soil moisture values were compared with in situ soil moisture upscaled from the four tested approaches as well as soil moisture estimated by the NOAH Land Surface Model (LSM). There were similar discrepancies when analyzing all methods (RMSE 0.072–0.074 m3 m−3 for the four upscaling methods; 0.076 m3 m−3 for the LSM approach), yielding no reduction in the soil moisture RMSE for this site. The SMAP team will continue to investigate other factors that may be contributing to errors above 0.04 m3 m−3 at these annually cropped CVSs.
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