•Paper firstly evaluate lipid oxidation process during freeze-thaw by ESR and Raman.•The main Raman intensity changes are 1655cm−1, I1655/I1745 and I1655/I1442.•The high correlations of conventional ...indexes with ESR and Raman were obtained.
In this study, electron spin resonance (ESR) and Raman spectroscopy were applied to characterize lipid oxidation of beef during repeated freeze–thaw (RFT). Besides the conventional indexes including peroxide values (PV), thiobarbituric acid-reactive substances (TBARS) and acid values (AV) were evaluated, the radical and molecular structure changes were also measured by ESR and Raman spectroscopy. The results showed that PV, TBARS and AV were increased (P<0.05) after RFT. This suggested that lipid oxidation was occurred during RFT. With the increase of radical signal intensity, lower oxidation stability was presented by ESR. Raman intensity of ν(CC) stretching region (1655cm−1) was decreased during RFT. Furthermore, lower Raman intensity ratio of I1655/I1442, I1655/I1745 that determine total unsaturation was also observed. Significant correlations (p<0.01) were obtained among conventional methods, ESR and Raman spectroscopy. Our result has proved that ESR and Raman spectroscopy showed great potential in characterizing lipid oxidation process of beef during RFT.
Primary pulmonary sarcoma (PPS) is a rare and poor prognostic malignancy that results from current clinical studies are lacking. Our study aimed to investigate the prognostic factors of PPS and to ...construct a predictive nomogram that predict the overall survival (OS) rate. We extracted data on patients diagnosed with PPS from 2010 to 2019 in the SEER database. A total of 169 patients were included after screening by inclusion and exclusion criteria. Univariate and multivariate COX regression analyses showed that age, pathological grade, liver metastasis, surgical intervention, and chemotherapy influenced the prognosis. We constructed the prediction model nomogram based on these factors. Moreover, the results of the internal and external ROC curves, calibration curves, and DCA plots confirmed that the model has good discrimination, accuracy, and clinical practice efficacy. The present study is the first population-based study to explore the factors affecting the prognosis of PPS. We established a novel prognostic nomogram to predict the OS rate, which can help to make proper clinical decisions.
Astragalus membranaceus is a traditional Chinese medicine derived from the roots of Astragalus membranaceus (Fisch.) Bge., which has the same medicinal and edible uses in China. It is also widely ...used in daily food, and its pharmacological effects mainly include antioxidant effects, vascular softening effects, etc. Currently, it is increasingly widely used in the prevention of hypertension, cerebral ischemia, and stroke in China. Formononetin and its glucopyranoside (ononin) are both important components of Astragalus membranaceuss and may play important roles in the treatment of cardiovascular diseases (CVDs). This study conducted metabolic studies using formononectin and its glucopyranoside (ononin), including a combination of the in vitro metabolism of Formonetin using rat liver S9 and the in vivo metabolism of ononin administered orally to rats. Five metabolites (Sm2, 7, 9, 10, and 12) were obtained from the solution incubated with formononetin and rat hepatic S9 fraction using chromatographic methods. The structures of the five metabolites were elucidated as (Sm2)6,7,4′-trihydroxy-isoflavonoid; (Sm7)7,4′-dihydroxy-isoflavonoid; (Sm9)7,8,4′-trihydroxy-isoflavonoid; (Sm10)7,8,-dihydroxy-4′-methoxy-isoflavonoid; and (Sm12)6,7-dihydroxy-4′-methoxy- isoflavonoid on the basis of UV, NMR, and MS data. Totally, 14 metabolites were identified via HPLC-DAD-ESI-IT-TOF-MSn analysis, from which the formononetin was incubated with rat hepatic S9 fraction, and the main metabolic pathways were hydroxylation, demethylation, and glycosylation. Then, 21 metabolites were identified via HPLC-DAD-ESI-IT-TOF-MSn analysis from the urine samples from SD rats to which ononin was orally administered, and the main metabolic pathways were glucuronidation, hydroxylation, demethylation, and sulfonation. The main difference between the in vitro metabolism of formononetin and the in vivo metabolism of ononin is that ononin undergoes deglycemic transformation into Formonetin in the rat intestine, while Formonetin is absorbed into the bloodstream for metabolism, and the metabolic products also produce combined metabolites during in vivo metabolism. The six metabolites obtained from the aforementioned separation indicate the primary forms of formononetin metabolism, and due to their higher contents of similar isoflavone metabolites, they are considered the main active compounds that are responsible for pharmacological effects. To investigate the metabolites of the active ingredients of formononetin in the rat liver S9 system, network pharmacology was used to evaluate the cardiovascular disease (CVD) activities of the six primary metabolites that were structurally identified. Additionally, the macromolecular docking results of six main components and two core targets (HSP90AA1 and SRC) related to CVD showed that formononetin and its main metabolites, Sm10 and Sm12, may have roles in CVD treatment due to their strong binding activities with the HSP90AA1 receptor, while the Sm7 metabolite may have a role in CVD treatment due to its strong binding activity with the SRC receptor.
Exercise-induced fatigue is a common physiological response to prolonged physical activity, often associated with changes in gut microbiota and metabolic responses. This study investigates the ...potential role of Brassica rapa L. in modulating these responses. Using an animal model subjected to chronic exercise-induced stress, we explored the effects of Brassica rapa L. on fatigue-related biomarkers, energy metabolism genes, inflammatory responses, intestinal integrity, and gut microbiota composition. Our findings revealed that Brassica rapa L. exhibits significant antioxidant activity and effectively modulates physiological responses to fatigue. It influences gene expression related to the tricarboxylic acid (TCA) cycle in muscle tissue through the AMPK/PGC-1α/TFAM signaling pathway. Furthermore, Brassica rapa L. has been found to alleviate inflammation by inhibiting lipopolysaccharide (LPS) infection and suppressing the activation of the NF-κB pathway. It also maintains intestinal integrity and controls Gram-negative bacterial growth. A correlation analysis identified several pathogenic bacteria linked with inflammation and energy metabolism, as well as beneficial probiotic bacteria associated with improved energy metabolism and reduced inflammation. These findings underscore Brassica rapa L.’s potential for managing prolonged exercise-induced fatigue, paving the way for future therapeutic applications. The results highlight its impact on gut microbiota modulation and its role in nutrition science and sports medicine.
The switching characteristics of HfOx-based resistive switching random access memory (RRAM) fabricated on front-end of 28 nm standard CMOS process are investigated. The integration of RRAM in the ...front-end of the CMOS process would minimize the influence of parasitic parameters caused by interconnection line and interlayer oxide on the one transistor one RRAM (1T1R) cells. By adopting pulse program-verify measurement for cycling test, we observe two nonideal phenomena in the commercialized RRAM devices for the first time: 1) low resistance state (LRS) current periodically decays exponentially accompanied by incremental SET pulse number and then increases abruptly when the cycle reaches a certain level and 2) high resistance state (HRS) current fluctuated dramatically during RESET operation. The current fluctuation is mainly due to the generation of new oxygen vacancies (Vos) near the bottom electrode and gap region by electrons trapping/detrapping. By adopting efficient pulse amplitude modulation, the optimized programming strategy has greatly reduced the switching instability and improved the endurance of the devices. Mechanisms of the instability and the optimization strategy are explained by a modified trap-assisted tunneling (TAT) model based on the generation and redistribution of Vos during cycling operations.
In this paper, we derive the Cramer-Rao bound (CRB) for joint target position and velocity estimation using an active or passive distributed radar network under more general, and practically ...occurring, conditions than assumed in previous work. In particular, the presented results allow nonorthogonal signals, spatially dependent Gaussian reflection coefficients, and spatially dependent Gaussian clutter-plus-noise. These bounds allow designers to compare the performance of their developed approaches, which are deemed to be of acceptable complexity, to the best achievable performance. If their developed approaches lead to performance close to the bounds, these developed approaches can be deemed "good enough". A particular recent study where algorithms have been developed for a practical radar application which must involve nonorthognal signals, for which the best performance is unknown, is a great example. The presented results in our paper do not make any assumptions about the approximate location of the target being known from previous target detection signal processing. In addition, for situations in which we do not know some parameters accurately, we also derive the mismatched CRB. Numerical investigations of the mean squared error of the maximum likelihood estimation are employed to support the validity of the CRBs. In order to demonstrate the utility of the provided results to a topic of great current interest, the numerical results focus on a passive radar system using the Global System for Mobile communication (GSM) cellar system.
COVID-19 vaccines emerging from different platforms differ in efficacy, duration of protection, and side effects. To maximize the benefits of vaccination, we explored the utility of employing a ...heterologous prime-boost strategy in which different combinations of the four types of leading COVID-19 vaccine candidates that are undergoing clinical trials in China were tested in a mouse model. Our results showed that sequential immunization with adenovirus vectored vaccine followed by inactivated/recombinant subunit/mRNA vaccine administration specifically increased levels of neutralizing antibodies and promoted the modulation of antibody responses to predominantly neutralizing antibodies. Moreover, a heterologous prime-boost regimen with an adenovirus vector vaccine also improved Th1-biased T cell responses. Our results provide new ideas for the development and application of COVID-19 vaccines to control the SARS-CoV-2 pandemic.
Developing a TiAlN-based hard coating with a multilayer structure comprising multiple nitride components shows potential to enhance the overall performance of high temperature applications. In this ...study, multi-target cathodic arc evaporation was utilized to deposit multilayer coatings comprising of nanostructured AlCrN/TiAlNbN and AlCrBN/TiAlNbSiN. The focus of this study is to analyze how the different coatings undergo structural changes due to the growth of oxide scale and diffusion processes that take place during oxidation when subjected to high temperatures of 900 °C, and the roles of B and Si addition in their oxidation resistance were examined. The deposited AlCrN/TiAlNbN coating showed a typical columnar structure with nanolayer stacking (average bilayer periodic thickness ~ 14.8 nm). The surface formed an oxide layer rich in titanium oxide, while the internal oxide layer of the AlCrN/TiAlNbN coating that underwent oxidation contained mixed metal oxides with a dominant composition of Al2O3, which hindered additional oxidation. The AlCrBN/TiAlNbSiN coatings, which had average bilayer periodic thickness 11.4 nm ~12.2 nm, showed fine-fibrous growth morphologies and refining effects of B and Si in the AlCrBN/TiAlNbSiN coatings, and they possessed better high temperature oxidation resistance thang that of AlCrN/TiAlNbN. Due to the formation of a protective oxidized layer with a combination of metal oxides, which minimizes the inward diffusion of oxygen during oxidation, the AlCrBN/TiAlNbSiN coating with a thicker AlCrBN layer exhibited the highest resistance to oxidation compared to the other coatings studied.
Display omitted
•AlCrBN/TiAlNbSiN multilayer coatings were deposited using cathodic arc evaporation.•Different oxidation behaviors of the multilayer coatings are studied.•The AlCrBN/TiAlNbSiN coatings showed fine-fibrous growth and refining effects.•A bilayered oxide scale was formed on the AlCrN/TiAlNbN after oxidation.•The multilayer structure of the AlCrBN/TiAlNbSiN is effective in preventing oxidation.
Increasing evidence indicates that renal recovery from AKI stems from dedifferentiation and proliferation of surviving tubule epithelial cells. Both EGF receptor (EGFR) and the Hippo signaling ...pathway are implicated in cell proliferation and differentiation, and previous studies showed that activation of EGFR in renal proximal tubule epithelial cells (RPTCs) plays a critical role in recovery from ischemia-reperfusion injury (IRI). In this study, we explored RPTC activation of Yes-associated protein (YAP) and transcriptional coactivator with PDZ binding motif (TAZ), two key downstream effectors of the Hippo pathway, and their potential involvement in recovery from AKI.
We used immunofluorescence to examine YAP expression in kidney biopsy samples from patients with clinical AKI and controls (patients with minimal change disease). Studies of RPTC activation of YAP and TAZ used cultured human RPTCs that were exposed to hypoxia-reoxygenation as well as knockout mice (with inducible deletions of
,
, or both occurring specifically in RPTCs) that were subjected to bilateral IRI.
YAP was activated in RPTCs in kidneys from post-AKI patients and post-IRI mouse kidneys. Inhibition of the interaction of YAP and the TEA domain (TEAD) transcription factor complex by verteporfin or conditional deletion of YAP in RPTCs delayed renal functional and structural recovery from IRI, whereas TAZ deletion had no effect. Activation of the EGFR-PI3K-Akt pathway in response to IRI signaled YAP activation, which promoted cell cycle progression.
This study shows that EGFR-PI3K-Akt-dependent YAP activation plays an essential role in mediating epithelial cell regeneration during kidney recovery from AKI.
•Biodegradable graphite/starch materials with high EM shielding were prepared.•Cation-π interaction improved delamination and dispersion of graphite in starch.•Orientation of graphite was also very ...important to EM shielding performance.•Cation-π interaction could enhance interfacial polarization of microwaves.•Orientation of graphite endowed the Salisbury screen effect.
Herein, a low-cost, biodegradable, and high-performance microwave shielding graphite/starch material was fabricated via constructing a cation-π interaction between ammonium ions and graphite. The graphite flakes and starch were firstly mixed with distilled water containing ammonium hydroxide to form graphite/starch slurry under an ultrasonic assistant. The cation-π interaction could improve delamination degree and dispersion of graphite in starch matrix. The slurry was first used as a coating material on the surface of wood and paper to develop shielding packages. The effect of coating thickness and coating layers on EM shielding property of the materials was investigated. Second, the composites with a high orientation of graphite were fabricated by compression at high pressures. The electrical conductivity and EM shielding effectiveness (SET) of the materials were greatly enhanced by construction of cation-π interaction and orientation of graphite. Specifically, the EM SET values increased from 56.9 to 66.8 dB for the composites with 50 wt.% graphite and 2.0 mm in thickness by constructing cation-π interaction. The EM SET values raised from 17.4 to 66.8 dB via the graphite orientation in the materials with the same components and thickness. The shielding mechanism of the compressed composites with orientation dispersion of graphite was also discussed in comparison to the coating layer with random dispersion of graphite.
Display omitted
PDF
