Since there are fewer studies on the integration of ideological and political education and educational management in colleges and universities at present, this paper combines digital technology to ...explore. After constructing the index system of the level of college civic education and education management respectively, the index system is normalized, and the coupling level of college civic education and education management is measured by combining the objective empowerment method. Based on the coupling path of the two, the SCIR model is introduced to construct the digital information dissemination model of DR-SCIR social network to explore the synergistic dissemination of the two. On this basis, taking colleges and universities in location S as an example, the digital communication mechanism of the two is analyzed while exploring the level of coupling between college civic education and education management. The results show that the coupling level between the level of college civic and political education and the level of education management are all above 0.9 during 2013-2022 in place S, which is in the highly coupled interval, while the coupling coordination degree in 2019 exceeds 0.5 for the first time. During the process of information dissemination rate increasing from 0.4 to 0.8, the peak value of the dissemination of the information dissemination state of the college’s civic and political education decreases by 0.04, which corresponds to the dissemination of the information dissemination state of the education management the peak value of dissemination of the state increases by 0.03. The paths of higher education, civic education, and education management compete to actively guide the overall development of students.
Scant attention has been paid to how risk perceptions of public health crises may affect people's mental health.
: The aims of this study are to (1) construct a conceptual framework for risk ...perception and depression of people in public health crises, (2) examine how the mental health of people in the crisis of Coronavirus Disease 2019 (COVID-19) is affected by risk perception and its associated factors, including distance perception of the crisis and support of prevention and control policies, and (3) propose policy recommendations on how to deal with psychological problems in the current COVID-19 crisis.
: Online questionnaire survey was implemented. A total of 6373 people visited the questionnaire online, 1115 people completed the questionnaire, and the number of valid questionnaires was 1081. Structural equation modeling was employed for data analysis.
: Risk perception and its associated factors significantly affect the mental health of people in public health crises. Specifically, (1) distance perception of public health crises is negatively associated with depression among people, (2) affective risk perception is positively associated with depression of people in public health crises, (3) cognitive risk perception is negatively associated with depression of people in public health crises, and (4) support of prevention and control policies is negatively associated with depression of people in public health crises.
: The findings of this study suggest that risk perception plays an important role in affecting the mental health of people in a public health crisis. Therefore, health policies aiming to improve the psychological wellbeing of the people in a public health crisis should take risk perception into consideration.
Herein, we report the densification behavior of a typical electric ceramic, barium titanate (BaTiO3), and highlight the role of oxygen vacancies in rapid densification of BaTiO3 during flash ...sintering. The activation energies of flash- and conventional-densification processes are compared to reveal that the initiation of flash sintering is triggered by the accumulation of generated oxygen vacancies under the electric field. Furthermore, the densification kinetics of BaTiO3 and X-ray photoelectron spectroscopy of flash-sintered BaTiO3 infer that the ultrafast densification during the flash-sintering stage is dominated by the migration of electric field-generated defects coupled with defect-annihilation by take-in oxygen from the environment.
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The mechanisms by which conductive and dielectric nanoparticles (NPs) trap electrons are explained by the potential well distribution caused by induced or polarized charges on NPs. Thus, the ...distributions of surface and saturation charges on conductive and dielectric NPs are determined. Given conductive Fe 3 O 4 , semiconductive TiO 2 , and dielectric Al 2 O 3 NPs, insulation performance tests are conducted and ionization models of nanofluids (NFs) based on transformer oil are developed. These models are compared with those of NFs based on pure oil. The NP whose conductivity or permittivity does not match that of the dielectric liquid has a potential well and an increased amount of saturation charges on its interface. This NP influences streamer development strongly and enhances the breakdown of oil-based NF.
Remote sensing image scene classification is essential, and it can promote the rational planning of land and ecological monitoring in the practical application of agricultural production. High ...spatial resolution (HSR) remote sensing images are widely used in smart agriculture because of their wide coverage and HSR. The HSR remote sensing images have a more detailed description of the local scene. However, the complexity of scene details intensifies the intraclass diversity and interclass similarity of scenes, and the interference to scene classification is more significant. To distinguish scene categories effectively in complex background, this article proposes a scene classification method of remote sensing images based on progressive aggregation (PA) with local and global cooperative learning. Specifically, multilevel local and global feature modules are employed at different levels to describe the influence of local objects and their global distribution on scene category determination. Then, the PA module is introduced to explore the collaboration of the same level features and reduce the interference of shallow redundancy. The residual structure can establish the correlation between multilevel representations, thereby improving the representation of the aggregate features. To verify the performance of the proposed method, we implemented cross-domain experiments on four internationally available remote sensing image classification datasets: NWPU-RESISC45, WHU-RS19, RSSCN7, and AID. The experimental results show that the proposed method is effective and robust in remote sensing scene classification.
The Guangdong-Hong Kong-Macau Greater Bay Area (GBA) has become a vital part of the national development strategy, where innovation and entrepreneurship play pivotal roles in its progress. In order ...to make meaningful contributions to the GBA’s advancement, regional colleges and universities must leverage the Internet’s advantages to drive educational and teaching reforms. Adopting an “Internet+” perspective, this research investigates the prospects and challenges encountered by GBA higher education institutions in the realm of innovation and entrepreneurship education. By drawing inspiration from successful international practices, it has proposed practical models encompassing the creation of online course platforms, the establishment of resource sharing systems, and the optimization of the innovation and entrepreneurial cloud ecosystem.
Advances in organoid culture technology have provided a greater understanding of disease pathogenesis, which has been rarely studied in sepsis before. We aim to establish a suitable organoids-based ...intestinal injury model for sepsis.
Stable passaged organoids were constructed and pre-treated with lipopolysaccharide (LPS) to mimic sepsis-induced intestinal injury. The LPS-induced sepsis model was used as a reference. We used quantitative real-time polymerase chain reaction to evaluate the RNA levels of inflammatory factors and antimicrobial peptides. Enzyme-linked immunosorbent assay was used to evaluate the protein levels, hematoxylin and eosin staining was used to evaluate the pathology of the small intestine of mice, and immunohistochemistry and immunofluorescence were used to evaluate the intestinal epithelial barrier function. Perkin Elmer Operetta™ was used to obtain high-resolution images of three-dimensional organoids.
An LPS concentration >150 μg/mL after 24 h was identified to cause organoid growth restriction. The fluorescence intensity of zonula occludens-1 and occludins at LPS concentrations >100 μg/mL decreased significantly after 24 h. After LPS stimulation for 8 h, the RNA expression levels of interleukin (IL)-1α, tumor necrosis factor alpha, granulocyte-macrophage colony-stimulating factor, IL-6, and regenerating islet-derived protein 3 alpha, beta, and gamma increased. These results resembled those of intestinal epithelial layer alterations in a mouse sepsis model. For IL-10, the RNA expression level increased only when the LPS level >200 μg/mL for 24 h.
This study provides the primary intestinal in vitro model to study the effects of LPS-induced intestinal injury resembling sepsis. This model provides a platform for immune associated mechanism exploration and effective drug screening.
Lentiviruses are increasingly used as gene delivery vehicles for vaccines and immunotherapies. However, the purification of clinical-grade lentivirus vectors for therapeutic use is still troublesome ...and limits preclinical and clinical experiments. Current purification methods such as ultracentrifugation and ultrafiltration are time consuming and do not remove all of the impurities such as cellular debris, membrane fragments, and denatured proteins from the lentiviruses. The same challenges exist in terms of their analytical characterization. Presented here is the novel demonstration of the chromatographic isolation of virus particles from culture media based on the hydrophobicity characteristics of the vesicles. A method was developed to isolate lentivirus from media using a hydrophobic interaction chromatography (HIC) method performed on a polyester, capillary-channeled polymer (PET C-CP) stationary phase and a standard liquid chromatography apparatus. The method is an extension of the approach developed in this laboratory for the isolation of extracellular vesicles (EVs). Quantitative polymerase chain reaction (qPCR) was used to verify and quantify lentiviruses in elution fractions. Load and elution mobile phase compositions were optimized to affect high efficiency and throughput. The process has been visualized via scanning electron microscopy (SEM) of the fiber surfaces following media injection, the elution of proteinaceous material, and the elution of lentiviruses. This effort has yielded a rapid (<10 min), low-cost (< $15 per column, providing multiple separations), and efficient method for the isolation/purification of lentivirus particles from cell culture media at the analytical scale.
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Bisphenol AF (BPAF) represents a common environmental estrogenic compound renowned for its capacity to induce endocrine disruptions. Notably, BPAF exhibits an enhanced binding affinity to estrogen ...receptors, which may have more potent estrogenic activity compared with its precursor bisphenol A (BPA). Notwithstanding, the existing studies on BPAF-induced prostate toxicity remain limited, with related toxicological research residing in the preliminary stage. Our previous studies have confirmed the role of BPAF in the induction of ventral prostatic hyperplasia, but its role in the dorsal lobe is not clear. In this study, BPAF (10, 90 μg/kg) and the inhibitor of nuclear transcription factor-κB (NF-κB), pyrrolidinedithiocarbamate (PDTC, 100 mg/kg), were administered intragastrically in rats for four weeks. Through comprehensive anatomical and pathological observations, as well as the assessment of PCNA over-expression, we asserted that BPAF at lower doses may foster dorsal prostatic hyperplasia in rats. The results of IHC and ELISA indicated that BPAF induced hyperplastic responses in the dorsal lobe of the prostate by interfering with a series of biomarkers in NF-κB signaling pathways, containing NF-κB p65, COX-2, TNF-α, and EGFR. These findings confirm the toxic effect of BPAF on prostate health and emphasize the potential corresponding mechanisms.
Cucurbitacin B (Cu B), a triterpenoid compound, has anti-inflammatory and antioxidant activities. Most studies only focus on the hepatoprotective activity of Cu B, and little effort has been geared ...toward exploring the effect of Cu B on the prostate. Our study identified that Cu B inhibited the proliferation of the benign prostatic hyperplasia epithelial cell line (BPH-1). At the molecular level, Cu B upregulated
and thrombospondin 1 (
) mRNA levels. Immunocytochemistry results revealed that the protein expressions of p53 and MDM2 were upregulated in BPH-1 cells. Furthermore, Cu B upregulated THBS1 expression and downregulated COX-2 expression in the BPH-1 cell supernatant. Altogether, Cu B may inhibit prostate cell proliferation by activating the p53/MDM2 signaling cascade and downregulating the COX-2 expression.
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