Bioelectrical impedance analysis (BIA) is a body composition assessment method. We aimed to determine its accuracy in the detection of sarcopenia in patients with liver cirrhosis (LC), using skeletal ...muscle index (SMI) at the level of third lumbar vertebra (L3-SMI) obtained using multislice computed tomography as the reference method. Patients with LC were enrolled in the period October 2019-March 2022 and follow-ups were conducted until January 2023. Their BIA parameters were compared against L3-SMI, and BIA cut-off values were proposed using AUROC analysis. Patients underwent outcome analysis based on obtained clinical characteristics. A total of 106 patients were included. We found a fair correlation between BIA parameters with the L3-SMI. We determined cut-off values of ≤11.1 kg/m
for BIA-SMI (Se 73%, Sp 66%, AUROC 0.737,
< 0.001) and ≤5.05° for phase angle (PA) (Se 79%, Sp 60%, AUROC 0.762,
< 0.001) in the detection of sarcopenia. The relative risk of death was 2.2 times higher in patients with skeletal muscle mass (SMM) ≤ 36.5 kg. SMM was significantly associated with outcome in Kaplan-Meier analysis. This non-invasive and simple method that showed fair performances and a very good outcome prediction could provide for the unmet need for fast and affordable detection of sarcopenia in patients with LC and should be further evaluated.
Gallbladder drainage is a treatment option in high-risk surgical patients with moderate or severe acute cholecystitis. It may be applied as a bridge to cholecystectomy or a definitive treatment ...option. Apart from the simple and widely accessible percutaneous cholecystostomy, new attractive techniques have emerged in the previous decade, including endoscopic transpapillary gallbladder drainage and endoscopic ultrasound-guided gallbladder drainage. The aim of this paper is to present currently available drainage techniques in the treatment of AC; evaluate their technical and clinical effectiveness, advantages, possible adverse events, and patient outcomes; and illuminate the decision-making path when choosing among various treatment modalities for each patient, depending on their clinical characteristics and the accessibility of methods.
Gastric cancer (GC) therapies include gastrectomy and chemoradiotherapy. The tumor immune microenvironment (TME) has implications for potential immunotherapy. We analyzed the expression of PD-L1, ...CD8, CTLA-4 and IFN-γ in the tumor and regional lymph node (LN) of patients with GC and compared it with clinical and pathological data. Paraffin blocks were collected from 97 patients undergoing gastrectomy/lymphadenectomy for GC. Double immunohistochemistry was performed for CD8 and PD-L1 and double immunofluorescence for CTLA-4 and IFN-γ. Statistical significance was set at
< 0.05. PD-L1 expression in tumor cells was associated with intestinal GC type (
= 0.046), the density of macrophages and CD8 + T cells (
< 0.001, both). The median number of CD8+ T cells was higher in PD-L1-positive than in -negative tumors. A cut-off of 28.5 CD8 + T cells in one high-magnification field predicted PD-L1-positive tumors (AUROC 0.797, sensitivity 74.2%, specificity 77.3%). IFN-γ expression in tumor cells was found in 37 GCs and was positively associated with CTLA4
lymphocytes in the LN (
= 0.027) and CTLA4
/IFN-γ+ in tumors and the LN (all
< 0.001). The median overall survival (OS) was 17 months. In the group of deceased patients, IFN-γ expression in metastases correlated with lower OS (RHO = -0.314,
= 0.008). PD-L1 expression in tumor cells correlated with CD8 + T cells and macrophages in the TME and IFN-γ expression with suppressive CTLA4
/IFNγ+ immune cells in the TME and LN.
A little knowledge exists about the probability that recombination in the parental maize populations will enhance the chances to select more stable genotypes. The synthetic parent maize population ...((1601/5 × ZPL913)F
2
= R
0
) with 25% of exotic germplasm was used to assess: (i) genotype × environment interaction and estimate stability of genotypes using nonparametric statistics; (ii) the effect of three (R
3
) and five (R
5
) gene recombination cycles on yield stability of genotypes; (iii) relationship among different nonparametric stability measures. The increase of mean grain yield was significant (
P
< 0.01) in the R
3
and R
5
in comparison to the R
0
, while it was not significant between R
3
and R
5
. Analysis of variance showed significant (
P
< 0.01) effects of environments, families per set, environment × set interaction, family × environment interaction per set on grain yield. The non-significant noncrossover and significant crossover (
P
< 0.01) G × (E) interactions were found according to Bredenkamp procedures and van der Laan-de Kroon test, respectively. The significant (
P
< 0.01) differences in stability were observed between R
0
-set 3 and R
5
-set 3 determined by
, R
3
-set 1 and R
5
-set 1 determined by
(
P
< 0.05), and R
0
-set 3 and R
5
-set 3 determined by
(
P
< 0.05). The significant parameters were those which take into account yield and stability so the differences could be due to differences in yield rather than stability. Findings can help breeders to assume the most optimum number of supplementary gene recombination to achieve satisfactory yield mean and yield stability of maize genotypes originating from breeding populations.
Acute ischemia/reperfusion (I/R) liver injury is a clinical condition challenging to treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation to less ...oxygen-consuming glycolysis. Thus, we investigated the effects of a 4-week meldonium pre-treatment (300 mg/kg b.m./day) on the acute I/R liver injury in Wistar strain male rats. Our results showed that meldonium ameliorates I/R-induced liver inflammation and injury, as confirmed by liver histology, and by attenuation of serum alanine- and aspartate aminotransferase activity, serum and liver high mobility group box 1 protein expression, and liver expression of Bax/Bcl2, haptoglobin, and the phosphorylated nuclear factor kappa-light-chain-enhancer of activated B cells. Through the increased hepatic activation of the nuclear factor erythroid 2-related factor 2, meldonium improves the antioxidative defence in the liver of animals subjected to I/R, as proved by an increase in serum and liver ascorbic/dehydroascorbic acid ratio, hepatic haem oxygenase 1 expression, glutathione and free thiol groups content, and hepatic copper-zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Based on our results, it can be concluded that meldonium represent a protective agent against I/R-induced liver injury, with a clinical significance in surgical procedures.
Climate factors have an impact on plant life cycle, yield, productivity, economy and profitability of agricultural production. There are not a lot of studies on understanding of influence of climate ...factors variation on maize yield in agro-ecological conditions of Serbia. The aim of this paper is analysis of variation of climatic factors over a long-time period, as well as assessment of impact of the examined climate parameters on maize yield in two localities in the Republic of Serbia. For the analysis of climatic factors (temperature, precipitation, sunshine, humidity) in the region of Central Serbia and Vojvodina, the data of meteorological stations Kragujevac and Sombor during two thirty-year periods (1961–1990 and 1991–2020) were used. In order to determine the existence and strength of the relationship between the observed climatic factors and maize yield, a correlation analysis of these indicators for the period 2005–2020 years, was performed. In the period 1991–2020, the average values of temperature were annually increased for 0.046 °C in Kragujevac and for 0.05 °C in Sombor, and in the same period the average value of sunshine on an annual level was increased for 1.3 h in Kragujevac and for 5.01 h in Sombor, 2020 in comparison to average values in period of 1961–1990. The humidity was decline annually for 1.3 in Kragujevac and for 3.4 in Sombor in period 1991–2020 in comparison to average humidity in period of 1961–1990. The results of the correlation analysis showed that the maize yield was significantly lower in the years with expressed high temperatures and precipitation deficit. Based on these studies, established effect of climate change on maize yield and that this demand developing adaptation agricultural practice through creating maize hybrids and varieties with greater adaptability and improvement of agrotechnic measure.
Heart failure (HF) with mid-range or mildly reduced ejection fraction (HFmrEF) is a separate clinical entity in the HF spectrum, with a left ventricular ejection fraction ranging from 40 to 49%. ...While sodium glucose co-transporter 2 inhibitors have become the cornerstone therapy for the entire HF spectrum, there are a few clinical trials of HFmrEF. This prospective observational study was conducted at Dubrava University Hospital, Zagreb, Croatia, from May 2021 to October 2023. We recruited 137 participants diagnosed with HFmrEF at admission. The majority were male, with a median age of 72 and overweight. A total of 110 participants were followed for 6 months and LVEF remained the same in the majority of patients (n = 62, 56.4%), improved in 32 patients (29.1%), and decreased in 3 patients (2.73%). A total of 64 participants were followed for 12 months: 39 remained the same (60.94%) and 25 improved. There were 13 deaths in (9.5%). While the empagliflozin group had a lower BMI at 6-month- and lower HbA1c at 12-month follow-up, there were no differences in death, HF hospitalizations, ER visits, or urinary tract infections in between groups. Despite recent and daily advances in the treatment of all HF phenotypes, HFmrEF still represents a challenge in everyday clinical practice.
Scholars have emphasised the importance of green settings in today’s business paradigms. Studies on green behaviour have produced a plethora of noteworthy discoveries, whether focused on financial ...success, individual capabilities, or development. However, despite significant growth in interest in green business practices, the relationship between individuals’ willingness and green competencies has received little attention. This article used the customised green competencies conceptual model to investigate how green skills influence organisational performance and their relationship with the willingness moment. This article developed an innovative human resource management approach to address these difficulties. A questionnaire was used to perform empirical statistical research with 516 respondents from Serbian universities. Different mathematical and statistical methodologies were used to analyse the results. The findings corroborate the suggested theoretical model, and they suggest that green competencies will influence people’s willingness to participate in green activities. This article gives new information on human behaviour and organisational effectiveness in a green atmosphere. It includes managerial and practical consequences and recommendations for businesses looking to improve their social responsibility and environmental sustainability.
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