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•Matured compost inoculation influenced sewage sludge composting properties.•Heating and volatile solid degradation rates in the thermophilic phase were reduced.•Matured compost ...increased enzyme contents during the mesophilic phase.•Bacterial diversity increased in the mesophilic phase with matured compost.•Matured compost affected correlations of microorganisms and enzyme activities.
The influence of matured compost inoculation during sewage sludge with sawdust composting was assessed. Mature compost reduced the heating rate, thermophilic phase, peak temperature, and volatile solid degradation rate, with no significant effect on pH and germination index. Matured compost addition also increased the cellulase, peroxidase, arylsulfatase, and urease contents during the mesophilic phase, and increased the urease content but decreased the cellulase, peroxidase, protease, and arylsulfatase contents during the cooling phase, with no significant effect on enzyme activities at the thermophilic phase. Matured compost increased the diversity of bacteria during the mesophilic and thermophilic phases, but reduced the fungal diversity throughout composting. Matured compost significantly improved uniformity of the bacterial community and affected the structure of the bacterial and fungal communities, while changing the correlation between some functional microorganisms and enzyme activities. These results provide guidance for optimizing the composting process when matured compost as bulking agent.
Ramie, Boehmeria nivea (L.) Gaudich, family Urticaceae, is a plant native to eastern Asia, and one of the world's oldest fibre crops. It is also used as animal feed and for the phytoremediation of ...heavy metal‐contaminated farmlands. Thus, the genome sequence of ramie was determined to explore the molecular basis of its fibre quality, protein content and phytoremediation. For further understanding ramie genome, different paired‐end and mate‐pair libraries were combined to generate 134.31 Gb of raw DNA sequences using the Illumina whole‐genome shotgun sequencing approach. The highly heterozygous B. nivea genome was assembled using the Platanus Genome Assembler, which is an effective tool for the assembly of highly heterozygous genome sequences. The final length of the draft genome of this species was approximately 341.9 Mb (contig N50 = 22.62 kb, scaffold N50 = 1,126.36 kb). Based on ramie genome annotations, 30,237 protein‐coding genes were predicted, and the repetitive element content was 46.3%. The completeness of the final assembly was evaluated by benchmarking universal single‐copy orthologous genes (BUSCO); 90.5% of the 1,440 expected embryophytic genes were identified as complete, and 4.9% were identified as fragmented. Phylogenetic analysis based on single‐copy gene families and one‐to‐one orthologous genes placed ramie with mulberry and cannabis, within the clade of urticalean rosids. Genome information of ramie will be a valuable resource for the conservation of endangered Boehmeria species and for future studies on the biogeography and characteristic evolution of members of Urticaceae.
Robots frequently need to work in human environments and handle many different types of objects. There are two problems that make this challenging for robots: human environments are typically ...cluttered, and the multi-finger robot hand needs to grasp and to lift objects without knowing their mass and damping properties. Therefore, this study combined vision and robot hand real-time grasp control action to achieve reliable and accurate object grasping in a cluttered scene. An efficient online algorithm for collision-free grasping pose generation according to a bounding box is proposed, and the grasp pose will be further checked for grasp quality. Finally, by fusing all available sensor data appropriately, an intelligent real-time grasp system was achieved that is reliable enough to handle various objects with unknown weights, friction, and stiffness. The robots used in this paper are the NTU 21-DOF five-finger robot hand and the NTU 6-DOF robot arm, which are both constructed by our Lab.
Aberrant expression of transforming growth factor‐β1 (TGF‐β1) is associated with renal cell carcinoma (RCC) progression by inducing cancer metastasis. However, the downstream effector(s) in TGF‐β ...signaling pathway is not fully characterized. In the present study, the elevation of secreted protein acidic and rich in cysteine (SPARC) as a TGF‐β regulated gene in RCC was identified by applying differentially expressed gene analysis and microarray analysis, we further confirmed this result in several RCC cell lines. Clinically, the expression of these two genes is positively correlated in RCC patient specimens. Furthermore, elevated SPARC expression is found in all the subtypes of RCC and positively correlated with the RCC stage and grade. In contrast, SPARC expression is inversely correlated with overall and disease‐free survival of patients with RCC, suggesting SPARC as a potent prognostic marker of RCC patient survival. Knocking down SPARC significantly inhibits RCC cell invasion and metastasis both in vitro and in vivo. Similarly, in vitro cell invasion can be diminished by using a specific monoclonal antibody. Mechanistically, SPARC activates protein kinase B (AKT) pathway leading to elevated expression of matrix metalloproteinase‐2 that can facilitate RCC invasion. Altogether, our data support that SPARC is a critical role of TGF‐β signaling network underlying RCC progression and a potential therapeutic target as well as a prognostic marker.
Perilla frutescens (L.) Britton is a classic herbal plant used widely against asthma in China. But its mechanism of beneficial effect remains undermined. In the study, the antiallergic asthma effects ...of Perilla leaf extract (PLE) were investigated, and the underlying mechanism was also explored. Results showed that PLE treatment significantly attenuated airway inflammation in OVA-induced asthma mice, by ameliorating lung pathological changes, inhibiting recruitment of inflammatory cells in lung tissues and bronchoalveolar lavage fluid (BALF), decreasing the production of inflammatory cytokines in the BALF, and reducing the level of immunoglobulin in serum. PLE treatment suppressed inflammatory response in antigen-induced rat basophilic leukemia 2H3 (RBL-2H3) cells as well as in OVA-induced human peripheral blood mononuclear cells (PBMCs). Furthermore, PLE markedly inhibited the expression and phosphorylation of Syk, NF-κB, PKC, and cPLA2 both in vivo and in vitro. By cotreating with inhibitors (BAY61-3606, Rottlerin, BAY11-7082, and arachidonyl trifluoromethyl ketone) in vitro, results revealed that PLE’s antiallergic inflammatory effects were associated with the inhibition of Syk and its downstream signals NF-κB, PKC, and cPLA2. Collectively, the present results suggested that PLE could attenuate allergic inflammation, and its mechanism might be partly mediated through inhibiting the Syk pathway.
Working memory is a process for short-term active maintenance of information. Behavioral neurophysiological studies in monkeys have demonstrated that the dorsolateral prefrontal cortex (dlPFC) is a ...key cortical region for working memory. The medial prefrontal cortex (mPFC) in rats is a cortical area similar to the dlPFC in monkeys in terms of anatomical connections, and is also required for behavioral performance on working-memory tasks. However, it is still controversial regarding whether and how mPFC neurons encode working memory. In the present study, we trained rats on a two-choice spatial delayed alternation task in Y maze, a typical working memory task for rodents, and investigated neuronal activities in the mPFC when rats performed the task. Our results show that, (1) inactivation of the mPFC severely impaired the performance of rats on the task, consistent with previous studies showing the importance of the mPFC for working-memory tasks; (2) 93.7% mPFC cells (449 in 479) exhibited changes in spiking frequency that were temporally locked with the task events, some of which, including delay-related cells, were tuned by spatial information; (3) differential delay activities in individual mPFC cells appeared transiently and sequentially along the delay, especially during the early phase of the delay; (4) some mPFC cells showed no change in discharge frequency but exhibited differential synchronization in firing during the delay. The present results suggest that mPFC neurons in rats are involved in encoding working memory, via increasing firing frequency or synchronization.
Sphingolipids, a type of bioactive lipid, play crucial roles within cells, serving as integral components of membranes and exhibiting strong signaling properties that have potential therapeutic ...implications in anti-cancer treatments. However, due to the diverse group of lipids and intricate mechanisms, sphingolipids still face challenges in enhancing the efficacy of different therapy approaches. In recent decades, mass spectrometry has made significant advancements in uncovering sphingolipid biomarkers and elucidating their impact on cancer development, progression, and resistance. Primary sphingolipids, such as ceramide and sphingosine-1-phosphate, exhibit contrasting roles in regulating cancer cell death and survival. The evasion of cell death is a characteristic hallmark of cancer cells, leading to treatment failure and a poor prognosis. The escape initiates with long-established apoptosis and extends to other programmed cell death (PCD) forms when patients experience chemotherapy, radiotherapy, and/or immunotherapy. Gradually, supportive evidence has uncovered the fundamental molecular mechanisms underlying various forms of PCD leading to the development of innovative molecular, genetic, and pharmacological tools that specifically target sphingolipid signaling nodes. In this study, we provide a comprehensive overview of the sphingolipid biomarkers revealed through mass spectrometry in recent decades, as well as an in-depth analysis of the six main forms of PCD (apoptosis, autophagy, pyroptosis, necroptosis, ferroptosis, and cuproptosis) in aspects of tumorigenesis, metastasis, and tumor response to treatments. We review the corresponding small-molecule compounds associated with these processes and their potential implications in cancer therapy.
A field experiment was conducted to assess the atmospheric deposition effects on lead (Pb) contamination in wheat by two contrasting treatments: wheat exposed or not to atmospheric deposition. Plants ...were housed in a shed during wheat greening for the non-exposed treatment. The Pb contents of wheat during different growth stages, of soil and of atmospheric deposits were analysed and combined with Pb stable isotope data to quantify the contribution of atmospheric deposition and soil to Pb in wheat tissue. The Pb content in atmospheric deposits was significantly higher than those in soil and wheat tissue, and the Pb content in wheat tissue exposed to atmospheric deposition was significantly higher than the Pb content in non-exposed tissue (p < 0.05). The 206Pb/207Pb of soil was significantly higher than the 206Pb/207Pb of atmospheric deposits (p < 0.05), and soil and atmospheric deposition were the two sources of Pb in wheat tissue. Atmospheric deposition was the main source of wheat tissue Pb in the exposed treatment, and most of the wheat tissue Pb, except for that in the stem, also came from atmospheric deposition in the maturing stage. The proportion of Pb from atmospheric deposition in roots, stems and leaves evidently decreased after the shed was erected, and the contribution of Pb from atmospheric deposition to wheat tissue was significantly higher in the exposed treatment than in the non-exposed treatment (p < 0.05). This contrast test directly confirmed that atmospheric deposition was the main source of Pb in the wheat tissues. Therefore, taking measures to reduce the absorption of Pb by wheat from atmospheric deposition can effectively ensure food safety.
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•Pb in wheat was source apportioned by contrast experiments based on isotopes.•Exposed wheat contained more atmospherically deposited Pb than non-exposed wheat.•Atmospheric deposition was the main source of Pb in wheat tissue.
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•eFeNSCs outperformed Pd and FeNSCs in the control of trichloroacetamide removal with high dechlorination selectivity.•Fe-N4 was the main active site for the adsorption of ...trichloroacetamide and C-Cl bond breaking.•Trichloroacetamide reduction followed a concerted mechanism with a 6-electron transfer.•High dechlorination selectivity of Fe-N4 sites gave the low genotoxicity of treated solution.
Herein, Fe/Co was firstly co-doped on zeolitic imidazolate framework-8 and then calcination to form electron-rich Fe-N4single site catalysts (eFeNSCs). TheeFeNSCs are composed of FeCo nanoparticles (∼5 nm) surrounded by Fe-N4active sites, giving the sites negatively charged. The electrochemicalin-situFourier transform infrared reflection (FTIR) spectroscopy and linear sweep voltammetry analysis demonstrate that theeFeNSCs promote the adsorption of trichloroacetamide and facilitate the C-Cl bond breaking, following a concerted mechanism. Over 97% trichloroacetamideremoval, 90% of dechlorination selectivity, and 37% of current efficiency were achieved oneFeNSCs within 90 min at −1.2 V, which are significantly superior to Fe-N4single site catalysts (FeNSCs) without FeCo nanoparticle. The high dechlorination selectivity of theeFeNSCs brought in the lower genotoxicity of the treated solution, which has been firstly demonstrated by SOS/umu tests. This study provides a new strategy in designing electron-rich single sites electrocatalysts for efficient elimination of disinfection by-products.
An efficient protocol for the ligand‐free and heterogeneous Pd/C‐catalyzed one‐pot, three‐component synthesis of aryl‐substituted benzimidazoles from benzyl alcohols by using water as the solvent was ...developed. The reaction involves hydrogen‐transfer and Suzuki reactions. This method is quite convenient and environmentally friendly.
An efficient protocol for the ligand‐free and heterogeneous Pd/C‐catalyzed one‐pot, three‐component synthesis of aryl‐substituted benzimidazoles from benzyl alcohols by using water as the solvent is developed. This method is quite convenient and environmentally friendly.
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