Previous studies have demonstrated the harmful effects of atmospheric pollutants on cardiac systems because of the presence of particulate matter (PM), a complex mixture of numerous substances ...including trace metals. In this study, the toxicity of PM2.5 from two regions, rural (PM2.5 level of 8.5 ± 4.0 μg m−3) and industrial (PM2.5 level of 14.4 ± 4.1 μg m−3) in Brazil, was investigated through in vivo experiments in rats. Metal accumulation and biochemical responses were evaluated after rats were exposed to three different concentrations of PM2.5 in saline extract (10× dilution, 5× dilution, and concentrated). The experimental data showed the bioaccumulation of diverse trace metals in the hearts of groups exposed to PM2.5 from both regions. Furthermore, mobilization of the antioxidant defenses and an increase in lipid peroxidation of the cardiac tissue was observed in response to the industrial and rural area PM2.5. Glutathione-S-transferase activity was increased in groups exposed to the 5× and concentrated rural PM2.5. Additionally, ATP-binding cassette (ABC) transporter activity in the cardiac tissue exposed to PM2.5 was reduced in response to the 5× dilution of the rural and industrial region PM2.5. Histological analysis showed a decrease in the percentage of cardiac cells in the heart at all tested concentrations. The results indicate that exposure to different concentrations of PM2.5 from both sources causes biochemical and histological changes in the heart with consequent damage to biological structures; these factors can favor the development of cardiac diseases.
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•Effects of fine particles and metals on cardiac system were evaluated.•Effects of PM2.5 levels between rural and urban areas were compared.•Metal accumulation, biochemical alterations, lipid damage, and morphological changes in rat heart were evaluated.•A possible pathway involved in triggering cardiac pathophysiologies promoted by air pollution was suggested.
The health effects of environmental chemicals on animals and humans are of growing concern. Human epidemiological and animal study data indicate that reproductive disorders and diseases begin early ...during prenatal and postnatal development. An increase of human male reproductive disturbance in the past several decades was associated to chemicals called endocrine disruptors (ED). Bisphenol A (BPA) is a ubiquitous organic environmental contaminant with ED activity. This study verified the effect of BPA exposure via breast milk during the lactation (early postnatal) period in male mice. Dams were exposed to oral BPA (300, 900, and 3000 µg/kg/BW/day) during the breastfeeding period (21 days). BPA at all concentrations significantly impaired sperm parameters in adult mice (8 months old), but mitochondrial functionality was more affected at BPA 3000. The acrosome membrane parameter was affected by BPA concentrations from 900 to 3000, and DNA integrity showed pronounced impairment at BPA 900 and 3000. BPA 3000 treatment also induced testicular degeneration and complete aplasia in some seminiferous tubules. Testicular oxidative damage was observed, and the total antioxidant capacity was impaired in BPA 900 and 3000 treatment groups. Taken together, the present study demonstrated long-term adverse effects of BPA in male mice, including reduced sperm quality, antioxidant capacity, and changes in testicular tissue. Our results clearly demonstrate the danger of BPA transferred via lactation on sperm quality registered even after a long time-elapsed from exposure to this harmful chemical.
Particulate matter (PM) contains different chemical substances that have been associated with health effects and an increased risk of mortality due to their toxicity. In this study, fine particulate ...matter (PM
2.5
) samples were collected in a region with rural characteristics (Seropédica (Se)) and another with some industries (Duque de Caxias (DC)) (Brazil, RJ). Rats were exposed to PM
2.5
extracts daily for 25 days at different dilutions: 10×, 5×, and a concentrated solution (CS). Biochemical analyses were investigated for total antioxidant capacity (ACAP), lipid peroxidation (LPO) levels, reduced glutathione (GSH) concentration, activity of glutamate cysteine ligase (GCL), and activity of glutathione S-transferase (GST). The liver showed a significant increase in GCL (DC-5×, DC-CS and Se-CS) and GST activities (DC-CS and Se-CS) in both regions when compared to the control group. In the renal cortex, GCL activity decreased in most of the tested groups while GST activity increased only in the 5× groups of both regions (DC and Se). In the renal medulla, GCL activity decreased for Se-10× and DC-CS but increased for Se-5×, and GST activity increased in the Se-10×, DC-5×, and DC-CS groups. Lung GCL increased in all groups for both regions. Moreover, this organ also showed an increase in GST activity when higher metal concentrations were present (5× and CS). TBARS levels were increased for all tissues in most tested concentrations. These data indicate that soluble compounds (e.g., metals) from PM
2.5
sampled in areas with different pollution indexes can change the redox status and cause damage to different tissues.
South American cyprinodontiform fish are potential candidates to be used as model biomarker species of exposure in environmental toxicology. The aim of this study was to identify molecular and ...biochemical biomarkers of pollution using Poecilia vivipara (Poecilidae) and Jenynsia multidentata (Anablepidae). Partial nucleotide sequences for cytochrome P-450 1A (CYP1A), a classical biomarker of exposure to organic contaminants in fish, were identified in P. vivipara and J. multidentata (approximately 650 nucleotides) using degenerated primers and polymerase chain reaction (PCR). These sequences shared approximately 90% identity in the predicted amino acid sequence with the corresponding CYP1A region of Fundulus heteroclitus. Real-time quantitative PCR (RT-qPCR) analysis confirmed that CYP1A transcription was markedly induced in the liver and gills of J. multidentata (approximately185-fold and 20-fold, respectively) and P. vivipara (122-fold and 739-fold, respectively) 24 h after exposure to 1 μM synthetic CYP1A inducer β-naphthoflavone (BNF). At 24 h after injection with 1 μg/g environmental carcinogenic contaminant benzoapyrene (BaP), a decreased total antioxidant capacity against peroxyl radicals was observed both in liver of J. multidentata and gills of P. vivipara. BaP injection in both fish did not produce changes in lipid peroxide (thiobarbituric acid-reactive substances, TBARS) levels, suggesting an absence of an oxidative stress condition. The newly identified CYP1A may thus serve as general biomarker of exposure to organic contaminant in future studies using P. vivipara and J. multidentata. Data also indicate the importance of species-specific differences in biomarker responses in these South American cyprinodontiform fish, suggesting distinct resistance/susceptibility properties to polycyclic aromatic hydrocarbons.
South American Cyprinodontiform fishes are potential candidates to be used as model species in environmental toxicology. We sought for molecular and biochemical biomarkers of pollution in
Poecilia ...vivipara
(Poecilidae) and
Jenynsia multidentata
(Anablepidae). Partial nucleotide sequences for the
cytochrome P450 1A
(
cyp1A
), a classical biomarker of exposure to organic contaminants in fish, were identified in
P. vivipara
and
J. multidentata
(∼ 650 nucleotides) using degenerated primers and PCR. These sequences shared ∼ 90 % identity in the predicted amino acid sequence with the corresponding Cyp1A region of
Fundulus heteroclitus
. RT-qPCR analysis confirmed that
cyp1A
transcription was strongly induced in the liver and gills of
J. multidentata
(∼185-fold and ∼20-fold, respectively) and
P. vivipara
(122-fold and 739-fold, respectively), after 24-hrs exposure to 1 μM of the synthetic
cyp1A
inducer β-naphthoflavone (BNF). After 24 hs of injection with 1 μg.g
-1
of the environmental carcinogenic contaminant benzoapyrene (BaP), a decreased total antioxidant capacity against peroxyl radicals was observed both in liver of
J. multidentata
and gills of
P. vivipara
. BaP injection in both fishes did not cause changes in lipid peroxides (TBARS) levels, suggesting an absence of an oxidative stress situation caused by BaP injection in this study. The newly identified
cyp1As
would serve as general biomarkers of exposure to organic contaminant in future studies using
P. vivipara
and
J. multidentata
. The results also points out to the important species-specific differences in the biomarker responses in those South American cyprinodontiform fishes, which would suggests distinct resistance/susceptibility to polycyclic aromatic hydrocarbons.
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