Hyper-plane-shaped clustering (HPSC) has been proved to be more effective in Takagi–Sugeno (T–S) fuzzy model identification compared with hyper-sphere-shaped clustering (HSSC). However, there is no ...special membership function matching HPSC in fuzzy modeling, and the commonly used bell-shaped Gaussian function is more suitable for HSSC. In this paper, a novel T–S fuzzy model identification method is adopted, in which a new fuzzy membership function designed for HSPC is designed. In this approach, a fuzzy c-regression model based clustering method is used to partition the fuzzy space firstly; and then a new HPSC fuzzy membership function is designed to identify the antecedent membership function (MF) parameters; finally the gravitational search algorithm is applied to optimize the MF parameters further. Experimental results on several benchmark problems show that modeling accuracies have been promoted significantly. The proposed approach has been applied in fuzzy modeling of pump-turbine governing system (PTGS). The comparative experimental results reveal that the proposed approach could achieve high accuracy and would be an effective modeling tool for complicated nonlinear system in engineering applications.
Among the various types of power supplies for electromagnetic launch, the inductive pulsed power supply (IPPS) has great research value and application prospects due to its high energy density. ...However, in the railgun system powered by IPPS, the energy flow has a redundant conversion process of magnetic-electric-magnetic, which may result in efficiency reduction. In this article, a novel configuration of a magnetic coupled railgun system is proposed, which allows the inductors of IPPS to provide an enhanced magnetic field for the railgun. It can increase the launching force, thereby improving the muzzle velocity and energy efficiency. The proposed system configuration is theoretically analyzed from the perspective of launch principle and circuit equivalence. It was also experimentally verified to be able to significantly improve the launch speed, and has little effect on the circuit operation and the mechanical structure of the launcher. In a set of verification experiments, the energy required by the magnetic coupled railgun system is only 47% of that of the conventional system, under the condition that the muzzle velocity is almost the same.
Ectopic ATP5B, which is located in a unique type of lipid raft caveolar structure, can be upregulated by cholesterol loading. As the structural component of caveolae, Cav-1 is a molecular hub that is ...involved in transmembrane signaling. In a previous study, the ATP5B-specific binding peptide B04 was shown to inhibit the migration and invasion of prostate cancer cells, and the expression of ATP5B on the plasma membrane of MDA-MB-231 cells was confirmed. The present study investigated the effect of ectopic ATP5B on the migration and invasion of MDA-MB-231 cells and examined the involvement of Cav-1. Cholesterol loading increased the level of ectopic ATP5B and promoted cell migration and invasion. These effects were blocked by B04. Ectopic ATP5B was physically colocalized with Cav-1, as demonstrated by double immunofluorescence staining and coimmunoprecipitation. After Cav-1 knockdown, the migration and invasion abilities of MDA-MB-231 cells were significantly decreased, suggesting that Cav-1 influences the function of ectopic ATP5B. Furthermore, these effects were not reversed after treatment with cholesterol. We concluded that Cav-1 may participate in MDA-MB-231 cell migration and invasion induced by binding to ectopic ATP5B.
The new energy vehicle industry needs a large number of vehicle power batteries, and its production depends on high-speed and high-precision battery pole-piece cutting equipment, which is mainly ...composed of the electrode material roll uncoiling unit, buffering unit, main drive unit, and cutting unit. Due to the nonlinearity and high-speed intermittent motion mode of a servomotor, the existing control technology is difficult to apply to the cutting system. This article presents a multimotor linear velocity planning approach used to ensure synchronous and cooperative operation of the motors in the cutting system. To achieve fast and accurate positioning control of the main drive motor running in fast acceleration and deceleration, periodic and intermittent mode, an adaptive position controller based on data-driven modeling technique is proposed, which makes the parameters of the controller change with the operation state of the main drive motor so that the controller can obtain good positioning accuracy in a wide working range of the main drive motor. Two linear models are established to represent the relationship between the positioning error and the positioning controller parameters and the main drive motor running conditions. The parameters of the two models are estimated offline and the parameters of the adaptive position controller can be obtained online quickly. Thus, the proposed control method for the main drive motor can be easily realized by general PLC in practical application. Simulation study results show that using the proposed method, the positioning error of the main drive motor is less than 0.1 mm when cutting 89-mm-long battery pole-piece and cutting 1 to 3 pieces/s. This result is much better than that of the position controller with fixed parameters.
According to the mine ground pressure and soil mechanics, the stress under a pillar is proportional to the coal pillar pressure and is in inverse proportion to the vertical depth and the pressure ...diffusion angle and is spreading along the gravity direction elliptically. In Tashan Coal Mine, by using UDEC, the stress distribution of lower seams was analyzed in three kinds of panels (panel 1, with coal unexcavated on both sides of the panel; panel 2, with coal unexcavated on one side and a pillar on the other side of the panel; and panel 3, with pillars on both sides of the panel). Numerical results show that (1) the area below the gob is a stress-reducing area and this stress is less than half of the original in situ stress and (2) the area below the pillar is a stress-increasing area and this stress is approximately 2.2∼2.8 times the stress of the primary rock, and the diffusion angle of the pillar floor is 18°∼20°. As a result, the roadway in the lower coal seam should be outside of the diffusion angle in order to avoid the influence of the stress-increasing area. The horizontal distance (starting from the edge of the upper pillar) at different depths (starting from the bottom of 2# coal) can be calculated. The roadway at the bottom of 3–5# coal (lower seam) should be set 7∼8 m outside of the upper coal pillar. The length of the panel in the lower coal seam should be approximately shorter than that of the upper panel.
In this work, isotactic polypropylene (iPP) was modified with an organic phosphate salt nucleating agent LPN-9081 and CaCO
3
. The synergistic effect of LNP-9801 and CaCO
3
in iPP was investigated ...using differential scanning calorimetry (DSC), scanning electronic microscope (SEM), and mechanical property tests. The DSC results showed that the peak crystallization temperature (Tc) of modified iPP was increased by about 10 °C when compared to that of pure iPP, this indicates that LNP-9801 and CaCO
3
increased the crystallization rate of iPP. The mechanical property tests demonstrated significant improvements in bending modulus and impact strength of modified iPP (impact strength increased by 33.9%, flexural modulus increased by 100.1%), which achieved an optimal balance of stiffness and toughness. Furthermore, the SEM results indicated that LPN-9081 contributed to the good dispersion of CaCO
3
in the iPP matrix. All of the above results show that organic phosphate salt nucleating agent LPN-9081 and CaCO
3
had good synergistic effects in iPP.
Graphical abstract
Diabetic retinopathy (DR) is the main cause of vision loss or blindness in working age adults worldwide. The lack of effective diagnostic biomarkers for DR leads to unsatisfactory curative ...treatments. To define potential metabolite biomarkers for DR diagnosis, a multiplatform‐based metabolomics study is performed. In this study, a total of 905 subjects with diabetes without DR (NDR) and with DR at different clinical stages are recruited. Multiplatform metabolomics methods are used to characterize the serum metabolic profiles and to screen and validate the DR biomarkers. Based on the criteria p < 0.05 and false‐discovery rate < 0.05, 348 and 290 metabolites are significantly associated with the pathogenesis of DR and early‐stage DR, respectively. The biomarker panel consisting of 12‐hydroxyeicosatetraenoic acid (12‐HETE) and 2‐piperidone exhibited better diagnostic performance than hemoglobin A1c (HbA1c) in differentiating DR from diabetes, with AUCs of 0.946 versus 0.691 and 0.928 versus 0.648 in the discovery and validation sets, respectively. In addition, this panel showed higher sensitivity in early‐stage DR detection than HbA1c. In conclusion, this multiplatform‐based metabolomics study comprehensively revealed the metabolic dysregulation associated with DR onset and progression. The defined biomarker panel can be used for detection of DR and early‐stage DR.
Multiplatform metabolomics methods are used to comprehensively investigate the association between the serum metabolic profiles and the onset and progression of diabetic retinopathy (DR) in a large Chinese population, and 12‐HETE and 2‐piperidone are identified as a highly effective marker panel for the detection of DR and early‐stage DR.
Chimeric antigen receptor (CAR) T-cell therapy has made remarkable progress in cancer immunotherapy, but several challenges with unclear mechanisms hinder its wide clinical application. Single-cell ...sequencing technologies, with the powerful unbiased analysis of cellular heterogeneity and molecular patterns at unprecedented resolution, have greatly advanced our understanding of immunology and oncology. In this review, we summarize the recent applications of single-cell sequencing technologies in CAR T-cell therapy, including the biological characteristics, the latest mechanisms of clinical response and adverse events, promising strategies that contribute to the development of CAR T-cell therapy and CAR target selection. Generally, we propose a multi-omics research mode to guide potential future research on CAR T-cell therapy.
Core saturation is a critical problem of high-power-level wireless charging. A coil-based desaturation strategy is proposed to desaturate the core without increasing the ferrite thickness or changing ...the original coil design. Planar coil and solenoids are connected in series with a capacitor to achieve a capacitive quasi-resonating status. The planar coil generates an enhancement magnetic field in phase with the transmitting field. The solenoids generate a canceling magnetic field with the phase opposite to its incident magnetic field from the ferrite. The desaturation strategy's principles, structure, and circuit analysis are explained and discussed. A scaled-down prototype is fabricated, and the desaturation effectiveness of the proposed strategy is verified both by finite element analysis (FEA) and experimental results.
Preeclampsia is one of the leading causes of maternal and perinatal morbidity and is characterized by hypertension, inflammation, and placental dysfunction. Gut microbiota plays key roles in ...inflammation and hypertension. However, its roles and mechanisms in preeclampsia have not been fully elucidated.
16S rRNA gene sequencing and targeted metabolomics were conducted on stool samples from 92 preeclamptic patients and 86 normal late-pregnant women. Then, fecal microbiota transplantation and in vitro and in vivo functional experiments were performed to explore the roles and mechanisms of gut microbiota in preeclampsia development.
We revealed the gut microbiota dysbiosis in preeclamptic patients, including significant reductions in short-chain fatty acid-producing bacteria and short-chain fatty acids. The gut microbiota of preeclamptic patients significantly exacerbated pathologies and symptoms of preeclamptic rats, whereas the gut microbiota of healthy pregnant women had significant protective effects.
, propionate, or butyrate significantly alleviated the symptoms of preeclamptic rats. Mechanistically, they significantly promoted autophagy and M2 polarization of macrophages in placental bed, thereby suppressing inflammation. Propionate also significantly promoted trophoblast invasion, thereby improved spiral arterial remodeling. Additionally, we identified a marker set consisting of
,
, and short-chain fatty acids that could accurately diagnose preeclampsia.
Our study revealed that gut microbiota dysbiosis is an important etiology of preeclampsia. Gut microbiota and their active metabolites have great potential for the treatment and diagnosis of preeclampsia. Our findings enrich the gut-placenta axis theory and contribute to the development of microecological products for preeclampsia.
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