IoT is leading a digital revolution in both academia and industry. It brings convenience to people's daily lives; however, the issues of security and privacy of IoT become challenges. Blockchain, a ...decentralized database based on cryptographic techniques, is promising for IoT security, which may influence a variety of areas including manufacture, finance, and trading. The blockchain framework in an IoT system is an intriguing alternative to the traditional centralized model, which is struggling to meet some specified demands in IoT. In this article, we investigate typical security and privacy issues in IoT and develop a framework to integrate blockchain with IoT, which can provide great assurance for IoT data and various functionalities and desirable scalability including authentication, decentralized payment, and so on. We also suggest some possible solutions to these security and privacy issues in IoT based on blockchain and Ethereum to show how blockchain contributes to IoT.
Image processing technology can provide necessary tools for the practice of environmental art courses. Especially in internationalization, the use of modern technologies such as image processing ...technology in environmental art courses can be in line with international standards and improve the quality. However, in the actual application, problems such as the unreasonable application mode of environmental art courses and the need to increase the use of image processing technology have arisen. This article will study the problems that arise in the technology in environmental art courses, and provide some opinions and suggestions on how to promote.
Human activities contribute greatly to heavy metal pollution in soils. Concentrations of 15 metal elements were detected in 105 soil samples collected from a typical rural-industrial town in southern ...Jiangsu, China. Among them, 7 heavy metals—lead, copper, zinc, arsenic, chromium, cadmium, and nickel—were considered in the health risk assessment for residents via soil inhalation, dermal contact, and/or direct/indirect ingestion. Their potential sources were quantitatively apportioned by positive matrix factorization using the data set of all metal elements, in combination with geostatistical analysis, land use investigation, and industrial composition analysis. Furthermore, the health risks imposed by sources of heavy metal in soil were estimated for the first time. The results indicated that Cr, Cu, Cd, Pb, Ni, and Co accumulated in the soil, attaining a mild pollution level. The total hazard index values were 3.62 and 6.11, and the total cancer risks were 9.78 × 10−4 and 4.03 × 10−4 for adults and children, respectively. That is, both non-carcinogenic and carcinogenic risks posed by soil metals were above acceptable levels. Cr and As require special attention because the health risks of Cr and As individually exceeded the acceptable levels. The ingestion of homegrown produce was predominantly responsible for the high risks. The potential sources were apportioned as: a) waste incineration and textile/dyeing industries (28.3%), b) natural sources (45.4%), c) traffic emissions (5.3%), and d) electroplating industries and livestock/poultry breeding (21.0%). Health risks of four sources accounted for 23.5%, 32.7%, 7.4%, and 36.4% of the total risk, respectively.
•Soil heavy metals caused unacceptable health risks, mainly through homegrown food.•Arsenic and chromium were the predominant hazardous elements.•Waste incineration, textile/dyeing industries were the main anthropogenic inputs.•Electroplating and livestock/poultry industries produced the highest health risks.
Cell wall biosynthesis is a complex biological process in plants. In the rapidly growing cells or in the plants that encounter a variety of environmental stresses, the compositions and the structure ...of cell wall can be dynamically changed. To constantly monitor cell wall status, plants have evolved cell wall integrity (CWI) maintenance system, which allows rapid cell growth and improved adaptation of plants to adverse environmental conditions without the perturbation of cell wall organization. Salt stress is one of the abiotic stresses that can severely disrupt CWI, and studies have shown that the ability of plants to sense and maintain CWI is important for salt tolerance. In this review, we highlight the roles of CWI in salt tolerance and the mechanisms underlying the maintenance of CWI under salt stress. The unsolved questions regarding the association between the CWI and salt tolerance are discussed.
Source-specific health risks of PM2.5-bound metals were analyzed for emission control by integrating source apportionment with health risk assessments of residents affected via inhalation pathways. A ...total of 218 daily PM2.5 samples were collected in 2016 in the central urban district of Beijing, China. Analyses showed that the mean annual concentrations of total heavy metals (THMs) and PM2.5 were 0.39 and 104.37 μg m−3, respectively. The heating season had significantly higher concentrations of THMs and PM2.5 (0.61, 134 μg m−3) than the non-heating season (0.27, 88.1 μg m−3) (p < 0.05). Among all metals, arsenic had the largest incremental cancer risk of 7.04 × 10−6. Six sources were identified by positive matrix factorization combined with conditional probability function and potential source contribution function analyses. The order of contribution to PM2.5-bound metal concentrations was resuspended dust (61.0%), traffic emission (16.3%), Cu-related industry (14.1%), coal combustion (3.7%), Cr-related industry (3.4%), and fuel oil combustion (1.6%). During the heating season, the contribution of coal combustion decreased slightly, which may have been due to the countermeasure of substituting coal for gas or electric heat in 2016. However, in terms of cancer risk contribution, coal combustion was the top contributor in both heating (3.5 × 10−6, 51.6%) and non-heating (2.7 × 10−6, 59.6%) seasons due to high attributable contents of the toxic metals, As, Cd and Pb. The Cr-related and Cu-related industries were the next controlled sources in the heating and non-heating seasons, respectively. Thus, these sources should receive priority in the development of control measures.
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•Individual PM2.5-bound heavy metals have higher concentrations in heating season.•Arsenic had the largest cancer risk, exceeding the acceptable level (1 × 10−6).•Six identical sources with seasonal contribution order were found by PMF-CPF-PSCF.•Resuspended dust contributed the greatest mass concentration.•With the highest cancer risk, coal combustion should be the priority control objective.
Accurately perceiving and predicting the parameters related to human walking is very important for man–machine coupled cooperative control systems such as exoskeletons and power prostheses. Plantar ...pressure data is rich in human gait and posture information and is an essential source of reference information as the input of the exoskeleton control system. Therefore, the proper design of the pressure sensing insole and validation is a big challenge considering the requirements such as convenience, reliability, no interference and so on. In this research, we developed a low-cost modular sensing unit based on the principle of photoelectric sensing and designed a plantar pressure sensing insole to achieve the purpose of sensing human walking gait and posture information. On the one hand, the sensor unit is made of economy-friendly commercial flexible circuits and elastic silicone, and the mechanical and electrical characteristics of the modular sensor unit are evaluated by a self-developed pressure-related calibration system. The calibration results show that the modular sensor based on the photoelectric sensing principle has fast response and negligible hysteresis. On the other hand, we analyzed the area where the plantar pressure is densely distributed. One benefit of the modular sensing unit design is that it is rather convenient to fabricate different insole solutions, so we fabricated and compared several pressure-sensitive insole solutions in this preliminary study. During the dynamic locomotion experiments of wearing the pressure-sensing insole, the time series signal of each sensor unit was collected and analyzed. The results show that the pressure sensing insole based on the photoelectric effect can sense the distribution of the plantar pressure by capturing the deformation of the insole caused by the foot contact during locomotion, and provide reliable gait information for wearable applications.
•Methane production was enhanced by both co-digestion and MW pretreatment.•The optimized ratio was 3:2 for anaerobic co-digestion based on MW pretreatment.•Propionic accumulation led to lower methane ...production of MW-FW compared to MW-SS.•Bacteroides only dominated in co-digestion, Methanosphaera only dominated in MW-FW.•Methanosarcina was the predominant methanogens for mono-SS and MW-SS.
The effects of microwave pretreatment (MW) on co-digestion of food waste (FW) and sewage sludge (SS) have never been investigated. In this study, a series of mesophilic biochemical methane potential (BMP) tests were conducted to determine the optimized ratio of FW and SS based on MW, and the evolution of bacterial and archaeal community was investigated through high-throughput sequencing method. Results showed that the optimized ratio was 3:2 for co-digestion of FW and SS based on MW, and the methane production was 316.24 and 338.44mLCH4/gVSadded for MW-FW and MW-SS, respectively. The MW-SS was superior for methane production compared to MW-FW, in which accumulation of propionic acid led to the inhibition of methanogenesis. Proteiniborus and Parabacteroides were responsible for proteins and polysaccharides degradation for all, respectively, while Bacteroides only dominated in co-digestion. Methanosphaera dominated in MW-FW at the active methane production phase, while it was Methanosarcina in MW-SS and mono-SS.
Modeling the processes of neuronal progenitor proliferation and differentiation to produce mature cortical neuron subtypes is essential for the study of human brain development and the search for ...potential cell therapies. We demonstrated a novel paradigm for the generation of vascularized organoids (vOrganoids) consisting of typical human cortical cell types and a vascular structure for over 200 days as a vascularized and functional brain organoid model. The observation of spontaneous excitatory postsynaptic currents (sEPSCs), spontaneous inhibitory postsynaptic currents (sIPSCs), and bidirectional electrical transmission indicated the presence of chemical and electrical synapses in vOrganoids. More importantly, single-cell RNA-sequencing analysis illustrated that vOrganoids exhibited robust neurogenesis and that cells of vOrganoids differentially expressed genes (DEGs) related to blood vessel morphogenesis. The transplantation of vOrganoids into the mouse S1 cortex resulted in the construction of functional human-mouse blood vessels in the grafts that promoted cell survival in the grafts. This vOrganoid culture method could not only serve as a model to study human cortical development and explore brain disease pathology but also provide potential prospects for new cell therapies for nervous system disorders and injury.
Integration of space resources (e.g., satellites) in different orbits with the terrestrial/ground network (e.g., internet) is the new evolving era of information technology. This kind of information ...network can expand the ground services such as internet to the whole world with the help of satellites' broader coverage. On the other hand, it can also provide realtime access to the satellite's useful data (e.g. images of earth), for nearly everyone on the globe. For these reasons, there has been enormous amount of research on this topic, and different network architectures are proposed for the integration of space and ground networks for different applications. However, most of them are either general abstract architecture or for the single dedicated application. Also for such a network, it is desirable that it should be based on the TCP/IP (Transmission control protocol/internet protocol) stack for interoperability with terrestrial networks. However, the biggest challenge for this is the dynamic network topologies due to orbital dynamics of space nodes. In this paper, we have addressed both of these issues by proposing a Space-Air-Ground Information Network (SAGIN) Architecture, which can provide almost all of the services of space and ground networks for all the users around the world according to varying needs of users. Our proposed SAGIN architecture is IP-based to provide interoperability and compatibility with the already deployed ground network. We have also addressed the challenge of dynamic nature of the network topology by designing a simple mechanism which automatically maintains a relatively static IPs of nodes in space (satellites), despite their rapid physical movement in orbits. This SAGIN architecture could be among optimized candidate architecture for future to have for many countries (either individually or by the cooperation of different regional countries). Because, it can provide all the services to nearly everyone in the world, in contrast to traditional individual satellites providing limited services to a limited number of users.
Summary
The clustered regularly interspaced short palindromic repeats‐associated protein 9 (CRISPR/Cas9) system is a powerful tool for editing plant genomes. Efficient genome editing of grape (Vitis ...vinifera) suspension cells using the type II CRISPR/Cas9 system has been demonstrated; however, it has not been established whether this system can be applied to get biallelic mutations in the first generation of grape. In this current study, we designed four guide RNAs for the VvWRKY52 transcription factor gene for using with the CRISPR/Cas9 system, and obtained transgenic plants via Agrobacterium‐mediated transformation, using somatic embryos of the Thompson Seedless cultivar. Analysis of the first‐generation transgenic plants verified 22 mutant plants of the 72 T‐DNA‐inserted plants. Of these, 15 lines carried biallelic mutations and seven were heterozygous. A range of RNA‐guided editing events, including large deletions, were found in the mutant plants, while smaller deletions comprised the majority of the detected mutations. Sequencing of potential off‐target sites for all four targets revealed no off‐target events. In addition, knockout of VvWRKY52 in grape increased the resistance to Botrytis cinerea. We conclude that the CRISPR/Cas9 system allows precise genome editing in the first generation of grape and represents a useful tool for gene functional analysis and grape molecular breeding.
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