Sanitation standard operating procedures (SSOP) are self-control tools used by the food industry to minimize contamination and ensure compliance with quality standards and safety for consumption. ...SSOPs are implemented in slaughterhouses to reduce or eliminate contamination during the slaughter stage. This work aimed to verify the microbiological effect of SSOP for washing beef carcasses, with pressurized (3 atm) and chlorinated (5 ppm) water from the hind to the front limb. In the forelimb region, groups of indicator microorganisms were quantified, and Salmonella spp., Listeria monocytogenes, pathotypes of diarrheagenic Escherichia coli non-0157, and Staphylococcus aureus with toxigenic potential were investigated in cattle carcasses slaughtered in northern Brazil before and after washing with SSOP. No significant differences (p > 0.05) were observed in the quantification of enterobacteria, mesophilic aerobes, total coliforms, E. coli, and S. aureus after washing with the SSOP. However, were detected Salmonella spp., Shiga toxin-producing (STEC), enteropathogenic (EPEC), and enterotoxigenic (ETEC) E. coli in the sampled carcass regions after washing. S. aureus, potentially producing enterotoxin D, was identified in two carcasses (6.66 %) before and after washing SSOP. Therefore, the SSOP may have spread contamination from other carcass areas to the forequarter region. Therefore, the processes, techniques, and SSOPs carried out at this stage must be reviewed, verified, and monitored to avoid the inclusion of pathogens that could compromise the microbiological safety of carcasses during the pre-washing stages.
This study developed a technique for the preparation of pâté from cachapinta (Pseudoplatystoma sp) waste. For this, frozen minced cachapinta fish was crushed in a mini cutter and homogenized with all ...other ingredients. The prepared pâté was stored in seamed and thermally treated cans (volume 170 g). Weight proportions of mean moisture, ash, protein, and lipid contents of the minced fish were 75.49, 1.00, 15.00, and 7.92 (g/100 g), respectively. The formulation of the developed pâté is in accordance with legislation for fish products. Cachapinta pâté is a product with high content of polyunsaturated fatty acids, low level of trans fat, and good indices of nutritional quality. Tests of sensory acceptance, purchase intent, and sensory attributes (except spreadability) averaged a score above 6.0, indicating acceptability of the product. Our study suggests that the potential of minced cachapinta for pâté production is high, and that it can contribute a value‐added product to seafood consumption.
Stages of development and nutritional and sensory evaluation of cachapinta (Pseudoplatystoma sp.) pâté
Nuclear and mitochondrial genes were used as molecular markers for verifying the iden-tity of fish fillets marketed as pintado (Pseudoplatystoma corruscans). Based on THE polymorphisms of nuclear DNA ...(RAG2, globin, and EF1 genes) and mitochondrial regions (16S), we examined whether the fillets originated from inbred species of pintado or from hybrids derived from crosses between cachara (P. reticulatum) and pintado. Nuclear genes from both species were detected in the analyzed fillets (n = 29). This clearly iden-tified these fish as interspecific hybrids (or F1/first filial generation) of the type “cacha-pinta,” resulting from a cross between female cachara and male pintado. These results demonstrate that monitoring fish fillet trading is crucial for detecting discrepancies between the marketed species and related information declared on the label. Species that are frequently hybridized, such as pintado and cachara, require special attention.
Nuclear and mitochondrial genes were used as molecular markers for verifying the iden-tity of fish fillets marketed as pintado (Pseudoplatystoma corruscans). Based on THE polymorphisms of nuclear DNA ...(RAG2, globin, and EF1 genes) and mitochondrial regions (16S), we examined whether the fillets originated from inbred species of pintado or from hybrids derived from crosses between cachara (P. reticulatum) and pintado. Nuclear genes from both species were detected in the analyzed fillets (n = 29). This clearly iden-tified these fish as interspecific hybrids (or F1/first filial generation) of the type “cacha-pinta,” resulting from a cross between female cachara and male pintado. These results demonstrate that monitoring fish fillet trading is crucial for detecting discrepancies between the marketed species and related information declared on the label. Species that are frequently hybridized, such as pintado and cachara, require special attention.
Nuclear and mitochondrial genes were used as molecular markers for verifying the iden-tity of fish fillets marketed as pintado (Pseudoplatystoma corruscans). Based on THE polymorphisms of nuclear DNA ...(RAG2, globin, and EF1 genes) and mitochondrial regions (16S), we examined whether the fillets originated from inbred species of pintado or from hybrids derived from crosses between cachara (P. reticulatum) and pintado. Nuclear genes from both species were detected in the analyzed fillets (n = 29). This clearly iden-tified these fish as interspecific hybrids (or F1/first filial generation) of the type “cacha-pinta,” resulting from a cross between female cachara and male pintado. These results demonstrate that monitoring fish fillet trading is crucial for detecting discrepancies between the marketed species and related information declared on the label. Species that are frequently hybridized, such as pintado and cachara, require special attention.
Nuclear and mitochondrial genes were used as molecular markers for verifying the iden-tity of fish fillets marketed as pintado (Pseudoplatystoma corruscans). Based on THE polymorphisms of nuclear DNA ...(RAG2, globin, and EF1 genes) and mitochondrial regions (16S), we examined whether the fillets originated from inbred species of pintado or from hybrids derived from crosses between cachara (P. reticulatum) and pintado. Nuclear genes from both species were detected in the analyzed fillets (n = 29). This clearly iden-tified these fish as interspecific hybrids (or F1/first filial generation) of the type “cacha-pinta,” resulting from a cross between female cachara and male pintado. These results demonstrate that monitoring fish fillet trading is crucial for detecting discrepancies between the marketed species and related information declared on the label. Species that are frequently hybridized, such as pintado and cachara, require special attention. --- Marcadores moleculares (PCR-Multiplex de genes nucleares e mitocondriais) foram uti-lizados para verificar a identidade molecular de filés de peixe comercializados como pintado (Pseudoplatystoma corruscans) com base em polimorfismos de regiões do DNA nuclear (genes RAG2, globina e EF1) e mitocondriais (16S) para verificar se os filés per-tenciam a espécie pura de pintado ou se eram híbridos derivados do cruzamento entre cachara (Pseudoplatystoma reticulatum) e pintado (Pseudoplatystoma corruscans). Os filés analisados (n = 29) apresentaram genes nucleares de ambas espécies P. corruscans e P. reticulatum, e desta forma, foram identificados como híbridos interespecíficos ou F1 (primeira geração filial) do tipo “cachapinta” resultante do cruzamento entre uma fêmea de cachara e um macho de pintado. Estes resultados mostram que o monitora-mento da comercialização de filés de peixe é fundamental para identificar situações onde existem diferenças entre as espécies comercializadas e as informações declaradas no rótulo. Espécies em que a hibridação tem sido frequentemente realizada como é o caso do pintado e da cachara, merecem atenção especial.
Nuclear and mitochondrial genes were used as molecular markers for verifying the iden-tity of fish fillets marketed as pintado (Pseudoplatystoma corruscans). Based on THE polymorphisms of nuclear DNA ...(RAG2, globin, and EF1 genes) and mitochondrial regions (16S), we examined whether the fillets originated from inbred species of pintado or from hybrids derived from crosses between cachara (P. reticulatum) and pintado. Nuclear genes from both species were detected in the analyzed fillets (n = 29). This clearly iden-tified these fish as interspecific hybrids (or F1/first filial generation) of the type “cacha-pinta,” resulting from a cross between female cachara and male pintado. These results demonstrate that monitoring fish fillet trading is crucial for detecting discrepancies between the marketed species and related information declared on the label. Species that are frequently hybridized, such as pintado and cachara, require special attention.
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