Viruses utilize host factors for their efficient proliferation. By evaluating the inhibitory effects of compounds in our library, we identified inhibitors of cyclophilin A (CypA), a known ...immunosuppressor with peptidyl-prolyl cis-trans isomerase activity, can significantly attenuate EV71 proliferation. We demonstrated that CypA played an essential role in EV71 entry and that the RNA interference-mediated reduction of endogenous CypA expression led to decreased EV71 multiplication. We further revealed that CypA directly interacted with and modified the conformation of H-I loop of the VP1 protein in EV71 capsid, and thus regulated the uncoating process of EV71 entry step in a pH-dependent manner. Our results aid in the understanding of how host factors influence EV71 life cycle and provide new potential targets for developing antiviral agents against EV71 infection.
According to the market survey and production measurement of Dafang County Agriculture and Animal Husbandry Bureau, the production of carrot reached 21 000 kg/ha, with income of 24 000 yuan/ha; the ...yield of white radish was 60 000 kg/ha, with income of 25 500 yuan/ha; the yield of cabbage was 45 000 kg/ha, with income of 36 000 yuan/ha; the yield of garlic sprout reached 2 250 kg/ha, with income of 18 000 yuan/ha; and the yield of garlic cloves was 4 500 kg/ha, with income of 15 750 yuan/ha. 3.2.4 Grassland + tourism. ...these restrict the application of agricultural machinery- Secondly, the farming system in the region is complex and diverse, which has brought certain difficulties to the development and promotion of agricultural machinery- In addition, factors such as the underdeveloped rural economy and the low quality of rural left-behind labor force have also brought varying degrees of influence on the application of mechanization. 3.3.2 Grassland agriculture having specialized and diversified requirements for agricultural machinery. ...influenced by traditional thought of generally adhering to the principle of not fighting for land with grain, forage grass crops are usually planted on hillsides , low-yield fields and autumn and winter idle fields, making mechanization application more difficult than major grain crops and economic crops. The same crop is required to be harvested in batches at diferent times for the needs of diferent uses such as green fodder and silage. ...factors such as diferent forage grass varieties, planting environments and harvesting periods have placed diferent special requirements on mechanization application, and also added difficulties for mechanization application. 3.3.3 Low mechanization performance of pasture and forage crops restricting the application of machinery- In terms of current performance of pasture machinery in China, compared with foreign developed countries, China's pasture machinery lacks uniform standards and complete series, most of which are single models.
In order to better promote the development of grassland husbandry in the Bijie experimental area and promote industrial poverty alleviation and rural revitalization strategies, the development status ...of grassland agriculture in developed countries (USA, Netherlands and France) and China is summarized. By learning from the successful experience of grassland agricultural development at home and abroad, combined with the survey results of grassland agriculture in Dafang County of Bijie experimental area, the development of grassland agriculture in Dafang County of Bijie experimental area is analyzed, and corresponding development strategies are put forward.
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