Noninvasive ventilation (NIV) is an increasingly used method of respiratory support. The use of NIV is expanding over the time and if properly applied, it can save patients' lives and improve ...long-term prognosis. However, both knowledge and skills of its proper use as life support are paramount. This systematic review aimed to assess the importance of NIV education and training. Literature search was conducted (MEDLINE: 1990 to June, 2018) to identify randomized controlled studies and systematic reviews with the results analyzed by a team of experts across the world through e-mail based communications. Clinical trials examining the impact of education and training in NIV as the primary objective was not found. A few studies with indirect evidence, a simulation-based training study, and narrative reviews were identified. Currently organized training in NIV is implemented only in a few developed countries. Due to a lack of high-grade experimental evidence, an international consensus on NIV education and training based on opinions from 64 experts across the twenty-one different countries of the world was formulated. Education and training have the potential to increase knowledge and skills of the clinical staff who deliver medical care using NIV. There is a genuine need to develop structured, organized NIV education and training programs, especially for the developing countries.
A study of the in vitro reconstitution of sugar beet cytochrome b ^sub 559^ of the photosystem II is described. Both α and β cytochrome subunits were first cloned and expressed in Escherichia coli. ...In vitro reconstitution of this cytochrome was carried out with partially purified recombinant subunits from inclusion bodies. Reconstitution with commercial heme of both (αα) and (ββ) homodimers and (αβ) heterodimer was possible, the latter being more efficient. The absorption spectra of these reconstituted samples were similar to that of the native heterodimer cytochrome b ^sub 559^ form. As shown by electron paramagnetic resonance and potentiometry, most of the reconstituted cytochrome corresponded to a low spin form with a midpoint redox potential +36 mV, similar to that from the native purified cytochrome b ^sub 559^. Furthermore, during the expression of sugar beet and Synechocystis sp. PCC 6803 cytochrome b ^sub 559^ subunits, part of the protein subunits were incorporated into the host bacterial inner membrane, but only in the case of the β subunit from the cyanobacterium the formation of a cytochrome b ^sub 559^-like structure with the bacterial endogenous heme was observed. The reason for that surprising result is unknown. This in vivo formed (ββ) homodimer cytochrome b ^sub 559^-like structure showed similar absorption and electron paramagnetic resonance spectral properties as the native purified cytochrome b ^sub 559^. A higher midpoint redox potential (+126 mV) was detected in the in vivo formed protein compared to the in vitro reconstituted form, most likely due to a more hydrophobic environment imposed by the lipid membrane surrounding the heme.PUBLICATION ABSTRACT
Reseñas de libros Anna Cacciola; José Rafael Valles Calatrava; Joaquín Rubio Tovar ...
Revista de literatura,
12/2019, Volume:
81, Issue:
162
Journal Article
Two Perromachias were published in Spain in the 18th Century: one by Francisco Nieto Molina (1765), the other by Juan Pisón y Vargas (1786). These mock-epic poems linked with the fable have been ...forgotten by critics. This paper focuses on Pisón y Vargas’ work and provides new information about his life. His Perromachia, written in eight silvas, takes Lope de Vegas’ Gatomaquia as main model, although the complete account of its sources shows that Pisón y Vargas gives includes in his poem the Parnasus of Spanish Golden Age that is in favour with the neoclassical canon: Garcilaso, Fray Luis and Villegas, particularly. The Perromachia is a moral fable, with footsteps of novel, and a satiric piece disguised as a text that continues the tradition of Golden Age mock-epic.
Objetivo. Evaluar el efecto antinociceptivo agudo y sobre la neuroconducta de la amitriptilina en dosis escalonadas. Materiales y métodos. Se utilizaron 82 ratones de aproximadamente 28 g, ...distribuidos en 10 grupos; se les suministró vía oral: agua destilada (0,1 mL/10 g), tramadol (20 mg/Kg), cafeína (32 mg/Kg), diazepam (32 mg/Kg)y amitriptilina en dosis escalonada (10-50 mg/Kg). Se emplearon las pruebas de la "placa caliente" y de Irwin. Se aplicaron las pruebas estadísticas de ANOVA, Tukey, coeficiente de correlación de Pearson y la exacta de Fisher. Resultados. La amitriptilina incrementó el umbral del dolor según el incremento de la dosis administrada, de 30 a 50 mg/Kg (p < 0,05; IC 95%). En las mismas dosis, se observó la presencia de sedación en 46,9%, 56,2%, y 81,2%, respectivamente. Conclusiones. Se demostró la acción antinociceptiva aguda de la amitriptilina y su relación dosis-respuesta en el rango de 30-50 mg/Kg, asimismo, se describieron manifestaciones neuroconductuales como: sedación, piloerección, incoordinación motora, salto, respiración anormal y marcha anormal.
A nonimmune library, containing single chain variable fragments (scFv) of immunoglobulin human genes displayed on the surface of M13 filamentous phages, was used to recognize molecules exposed on ...Histoplasma capsulatum yeasts' surface, during their growth in synthetic medium. The scFv clones were checked in their consistency by Dot-ELISA using HRP/anti-M13 conjugate, and they were tested to recognize molecules on H. Capsulatum yeasts' surface by ELISA in plates. Three out of 80 scFv cones (C2, C6, and C52) reacted consistently with H. capsulatum molecules, and they recognized molecules from both H. capsulatum morphologic phases. However, C6 and C52 clones reacted better with molecules on the surface of whole yeasts, with molecules from the yeasts' cell-wall extract, and with molecules released to the supernatant of the yeast culture. Mycelial supernatants from other fungi, as well as from a Mycobacterium filtrate, were not recongnized by scFv phage monoclones. Monoclones C2, C6, and C52 recognized yeast molecules irrespective of the H. capsulatum strains used; the C6 clone revealed a specific immunohistochemistry reaction when tested against homologous and heterologous fungal infected tissues. The scFv clones isolated will be a useful toll to define the role of their target molecules in the host-parasite relationship of histoplasmosis.
En el siglo xviii se publicaron dos Perromachias debidas a Francisco Nieto Molina (1765) y Juan Pisón y Vargas (1786), poemas épicos burlescos emparentados con la fábula a los que no se ha prestado ...atención crítica. Este artículo se centra en la obra de Pisón y Vargas, del que se ofrecen datos biográficos inéditos. Su Perromachia, distribuida en ocho silvas, toma como modelo principal La Gatomaquia de Lope de Vega, aunque en el rastreo exhaustivo de fuentes que acometemos puede verse que el espíritu neoclásico del autor da cabida en sus versos a citas y ecos del Parnaso áureo de las preferencias del siglo ilustrado: Garcilaso, Fray Luis y Villegas, principalmente. Se trata, en fin, de una fábula moral, con visos de novela y sátira de costumbres, bajo el disfraz de un texto que continúa la tradición de la épica burlesca de la Edad de Oro.
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