Advanced High Strength Steels (AHSS) are increasingly used in automotive industry due to their superior strength and substantial weight advantage. However, their compromised ductility gives rise to ...numerous manufacturing issues. One of them is the so-called ‘shear fracture’ often observed on tight radii during stamping processes. Since traditional approaches, such as the Forming Limit Diagram (FLD), are unable to predict this type of fractures, great efforts have been made to develop failure criteria that could predict shear fractures. In this paper, a recently developed Modified Mohr–Coulomb (MMC) ductile fracture criterion (
Bai and Wierzbicki, 2010) is adopted to analyze the failure behavior of a Dual Phase (DP) steel sheet during stretch-bending operations. The plasticity and ductile fracture of the present sheet are fully characterized by a Hill’48 orthotropic model and a MMC fracture model, respectively. Finite element models with three different element types (3D, shell and plane strain) were built for a Stretch Forming Simulator (SFS) test (
Shih and Shi, 2008), numerical simulations with four different
R/
t values (die radius normalized by sheet thickness) were performed. It has been shown that the 3D and shell element simulations can predict failure location/mode, the upper die load–displacement responses as well as wall stress and wrap angle at the onset of fracture for all
R/
t values with good accuracy. Furthermore, a series of parametric studies were conducted on the 3D element model, and the effect of tension level (clamping distance), tooling friction, mesh size and fracture locus on failure modes and load–displacement responses were investigated.
...I applied Cochrane Review Manager (RevMan, version 5.4) 2 with the random effect model to recalculate the 95%CI and p-value. According to Cochrane Handbook for Systematic Reviews of Interventions ...4, it is appropriate to use the mean difference (MD), not the standardized mean difference (SMD), as summary statistics for continuous data with the same scale. ...SMD is unitless, solely showing the difference in a relatively measurement scale, not a real difference in variability 4. ...the authors did not show theses plots in the main text or supplemental figures as well. ...to display the assessment of publication bias, funnel plots might better be added or mentioned.
Circulating tumor cells (CTCs) are tumor cells that have sloughed off the primary tumor and extravasate into and circulate in the blood. Understanding of the metastatic cascade of CTCs has tremendous ...potential for the identification of targets against cancer metastasis. Detecting these very rare CTCs among the massive blood cells is challenging. However, emerging technologies for CTCs detection have profoundly contributed to deepening investigation into the biology of CTCs and have facilitated their clinical application. Current technologies for the detection of CTCs are summarized herein, together with their advantages and disadvantages. The detection of CTCs is usually dependent on molecular markers, with the epithelial cell adhesion molecule being the most widely used, although molecular markers vary between different types of cancer. Properties associated with epithelial-to-mesenchymal transition and stemness have been identified in CTCs, indicating their increased metastatic capacity. Only a small proportion of CTCs can survive and eventually initiate metastases, suggesting that an interaction and modulation between CTCs and the hostile blood microenvironment is essential for CTC metastasis. Single-cell sequencing of CTCs has been extensively investigated, and has enabled researchers to reveal the genome and transcriptome of CTCs. Herein, we also review the clinical applications of CTCs, especially for monitoring response to cancer treatment and in evaluating prognosis. Hence, CTCs have and will continue to contribute to providing significant insights into metastatic processes and will open new avenues for useful clinical applications.
Small interfering RNA (siRNA) is an effective therapeutic to regulate the expression of target genes in vitro and in vivo. Constructing a siRNA delivery system with high serum stability, especially ...responsive to endogenous stimuli, remains technically challenging. Herein we develop anti-degradation Y-shaped backbone-rigidified triangular DNA bricks with sticky ends (sticky-YTDBs) and tile them onto a siRNA-packaged gold nanoparticle in a programmed fashion, forming a multi-functional three-dimensional (3D) DNA shell. After aptamers are arranged on the exterior surface, a biocompatible siRNA-encapsulated core/shell nanoparticle, siRNA/Ap-CS, is achieved. SiRNAs are internally encapsulated in a 3D DNA shell and are thus protected from enzymatic degradation by the outermost layer of YTDB. The siRNAs can be released by endogenous miRNA and execute gene silencing within tumor cells, causing cell apoptosis higher than Lipo3000/siRNA formulation. In vivo treatment shows that tumor growth is completely (100%) inhibited, demonstrating unique opportunities for next-generation anticancer-drug carriers for targeted cancer therapies.
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