The ability of light to carry and deliver orbital angular momentum (OAM) in the form of optical vortices has attracted much interest. The physical properties of light with a helical wavefront can be ...confined onto two-dimensional surfaces with subwavelength dimensions in the form of plasmonic vortices, opening avenues for thus far unknown light-matter interactions. Because of their extreme rotational velocity, the ultrafast dynamics of such vortices remained unexplored. Here we show the detailed spatiotemporal evolution of nanovortices using time-resolved two-photon photoemission electron microscopy. We observe both long- and short-range plasmonic vortices confined to deep subwavelength dimensions on the scale of 100 nanometers with nanometer spatial resolution and subfemtosecond time-step resolution. Finally, by measuring the angular velocity of the vortex, we directly extract the OAM magnitude of light.
Most hydrogenases (H₂ases), the enzymes that produce or oxidize dihydrogen, possess dimetallic active sites and belong to either one of two phylogenetically distinct classes, the NiFe and the FeFe ...H₂ases. These families of H₂ases share a number of similarities regarding active site structure and reaction mechanism, as a result of convergent evolution. They are otherwise alien to each other, in particular with respect to protein sequence and structure, maturation mechanisms, and distribution among the realms of life. One of the interesting features of FeFe H₂ases is their occurrence in anaerobic bacteria, anaerobic protists, and mitochondriate eukaryotes. They thus have the potential to report on important evolutionary events, including transitions from the prokaryote to the eukaryote lifestyle. Genome sequences yield a variety of FeFe H₂ase sequences that have been implemented to shed light on the evolution of these proteins and their host organisms.
Recently, the suspension of hybrid nanoparticles in conventional fluids has been investigated as a technique for improving the thermophysical properties of nanofluids. The dearth of documentation on ...the trio influence of volume concentration, base fluid, and temperature on the electrical conductivity and viscosity of hybrid alumina–ferrofluids Al
2
O
3
–Fe
2
O
3
(25:75 mass%) has led to this study. The effective viscosity and electrical conductivity of the deionized water (DW)-based and ethylene glycol (EG)–DW-based (50:50 vol%) hybrid alumina–ferrofluids were measured at temperatures of 20–50 °C and volume concentrations of 0.05–0.75%. Based on the importance of soft computing methods to engineers, adaptive neuro-fuzzy inference system (ANFIS) and artificial neural network (ANN) were used for predicting the relative viscosity and electrical conductivity of the two types of hybrid ferrofluids. The measured data for viscosity and electrical conductivity were used in the modeling. Model performances were evaluated using the root mean squared error index. Viscosity was enhanced by 3.23–43.64% and 2.79–49.38%, while electrical conductivity was increased by 163.37–1692.16% and 717.14–7618.89% for the DW- and EG–DIW-based hybrid ferrofluids, respectively, compared with the respective base fluids. Increasing volume concentration augmented the viscosity and electrical conductivity of all the hybrid alumina–ferrofluids, whereas a rise in temperature enhanced their electrical conductivity and detracted the viscosity. DW-based hybrid alumina–ferrofluid was observed to have a lower viscosity and higher electrical conductivity than the EG–DW-based counterpart. The results showed that the optimum ANN and ANFIS models have a maximum error of less than 4.5% and 3.9% for relative viscosity and electrical conductivity, respectively, which were lower than those proposed using regression analysis. With the hybrid alumina–ferrofluids possessing a lower viscosity relative to single-particle ferrofluids, they are recommended for engineering application.
Success with genome editing by the RNA-programmed nuclease Cas9 has been limited by the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have been successful, and ...even those that were, varied widely in their efficacy. Here we describe and validate a strategy for Caenorhabditis elegans that reliably achieved a high frequency of genome editing for all targets tested in vivo. The key innovation was to design guide RNAs with a GG motif at the 3' end of their target-specific sequences. All guides designed using this simple principle induced a high frequency of targeted mutagenesis via nonhomologous end joining (NHEJ) and a high frequency of precise DNA integration from exogenous DNA templates via homology-directed repair (HDR). Related guide RNAs having the GG motif shifted by only three nucleotides showed severely reduced or no genome editing. We also combined the 3' GG guide improvement with a co-CRISPR/co-conversion approach. For this co-conversion scheme, animals were only screened for genome editing at designated targets if they exhibited a dominant phenotype caused by Cas9-dependent editing of an unrelated target. Combining the two strategies further enhanced the ease of mutant recovery, thereby providing a powerful means to obtain desired genetic changes in an otherwise unaltered genome.
Concerning their sensitivity to ocean acidification, coccolithophores, a group of calcifying single-celled phytoplankton, are one of the best-studied groups of marine organisms. However, in spite of ...the large number of studies investigating coccolithophore physiological responses to ocean acidification, uncertainties still remain due to variable and partly contradictory results. In the present study we have used all existing data in a meta-analysis to estimate the effect size of future pCO2 changes on the rates of calcification and photosynthesis and the ratio of particulate inorganic to organic carbon (PIC / POC) in different coccolithophore species. Our results indicate that ocean acidification has a negative effect on calcification and the cellular PIC / POC ratio in the two most abundant coccolithophore species: Emiliania huxleyi and Gephyrocapsa oceanica. In contrast, the more heavily calcified species Coccolithus braarudii did not show a distinct response when exposed to elevated pCO2/reduced pH. Photosynthesis in Gephyrocapsa oceanica was positively affected by high CO2, while no effect was observed for the other coccolithophore species. There was no indication that the method of carbonate chemistry manipulation was responsible for the inconsistent results regarding observed responses in calcification and the PIC / POC ratio. The perturbation method, however, appears to affect photosynthesis, as responses varied significantly between total alkalinity (TA) and dissolved inorganic carbon (DIC) manipulations. These results emphasize that coccolithophore species respond differently to ocean acidification, both in terms of calcification and photosynthesis. Where negative effects occur, they become evident at CO2 levels in the range projected for this century in the case of unabated CO2 emissions. As the data sets used in this meta-analysis do not account for adaptive responses, ecological fitness and ecosystem interactions, the question remains as to how these physiological responses play out in the natural environment.
Bangladesh is one of the most flood-affected countries in the world. In the last few decades, flood frequency, intensity, duration, and devastation have increased in Bangladesh. Identifying ...flood-damaged areas is highly essential for an effective flood response. This study aimed at developing an operational methodology for rapid flood inundation and potential flood damaged area mapping to support a quick and effective event response. Sentinel-1 images from March, April, June, and August 2017 were used to generate inundation extents of the corresponding months. The 2017 pre-flood land cover maps were prepared using Landsat-8 images to identify major land cover on the ground before flooding. The overall accuracy of flood inundation mapping was 96.44% and the accuracy of the land cover map was 87.51%. The total flood inundated area corresponded to 2.01%, 4.53%, and 7.01% for the months April, June, and August 2017, respectively. Based on the Landsat-8 derived land cover information, the study determined that cropland damaged by floods was 1.51% in April, 3.46% in June, 5.30% in August, located mostly in the Sylhet and Rangpur divisions. Finally, flood inundation maps were distributed to the broader user community to aid in hazard response. The data and methodology of the study can be replicated for every year to map flooding in Bangladesh.
The emergence of nanofluids as high-performance thermal transport media has drawn great research attention in the field of heat transfer. Owning to the huge importance of natural convection ...applications in environmental, agricultural, manufacturing, electronics, aviation, power plants, and industrial processes, heat transfer and flow characteristics of these special fluids in various cavities have been extensively researched. This review paper has paid serious attention to the benefits of controlling the natural convection heat transfer and flow performance of nanofluids in square cavities using magnetic field sources in addition to the aspect ratio, porous media, cavity and magnetic field inclination, hybrid nanofluids, etc. The influence of several variables such as heat distribution methods, thermal and concentration boundary conditions, governing parameters, magnetic field types, numerical schemes, thermophysical correlation types, nanofluid types, slip conditions, Brownian motion, and thermophoresis on the magnetohydrodynamic (MHD) natural convection behaviours of nanofluids in square cavities has been reviewed. The paper focused on the application of numerical and experimental methods to hydromagnetic behaviours of nanofluids in square-shaped enclosures. The concept of bioconvection, bio-nanofluid (green nanofluid), ionic nanofluid, and hybrid nanofluid has also been reviewed in relation to natural convection for the first time. Special cases of MHD natural convection in cavities involving micropolar and hybrid nanofluids are also presented herein. Convective heat transfer in square cavities has been demonstrated to be altered due to the presence of magnetic fields.
![[FeFe] hydrogenases and the...](http://api.summon.serialssolutions.com/2.0.0/image/issn/XR4PS5YY4K/1420-682X/small "[FeFe] hydrogenases and their evolution: a genomic perspective")
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