The aim of this study was to gain insight into those proteins that might be involved in the early stages of liver fat accumulation as a consequence of a different fat versus simple sugar dietary ...intake.
Forty-five male Wistar rats were randomly distributed into four dietary groups: a starch-rich control diet (CD; n = 10), a high-fat diet (n = 12), a high-sucrose diet (n = 11), and a high-fat sucrose diet (HFSD; n = 12) for 5 weeks. A comparative analysis by 2D-DIGE and LC-ESI-MS/MS was performed to characterize the liver protein expression profiles due to the three obesogenic diets.
Ten out of 17 proteins whose expression levels were altered by >1.25-fold were identified. Four proteins (Hspa8, Hspa9, Ca3, and Cat) were differentially expressed after the HFSD period compared to CD. The heat shock proteins (Hspa8 and Hspa9) resulted significantly downregulated in liver from rats fed HFSD versus CD (p < 0.05). The results were confirmed by Western blot.
This descriptive study might be useful for further studies aiming at understanding the mechanisms by which diets rich in both fat and sugar affect the initiation of hepatic steatosis.
In this paper, the subgenus Amadotrogus Reitter, 1902 and its taxonomic status as a subordinate taxon of genus Amphimallon are reviewed. Two kinds of character sets are discussed; those relative to ...the external morphology of the adult and those of the male and female genitalia. Subgenus Amadotrogus is compared with its recognized allied genera in the same clade (Coca-Abia 1995) Amphimallon Berthold, 1827; Geotrogus Guérin, 1842; Monotropus Erichson, 1847; Pseudoapeterogyna Escalera, 1914 and Rhizotrogus Berthold, 1827 to asses the realtionships of these taxa. Phylogenetic analysis discloses that Amadotrogus, considered to be a subgenus of Amphimallon, has synapomorphic characters which justify its elevation to generic rank. Thus, genus Amadotrogus includes seven species distributed across the Northern Mediterranean basin. The type species, Amadotrogus quercanus (Burmeister, 1855), and six others, transferred from genus Rhizotrogus: Amadotrogus grassii (Mainardi, 1902), Amadotrogus insubricus (Burmeister, 1855), Amadotrogus oertzeni (Brenske, 1886), Amadotrogus patru- elis (Reiche, 1862), Amadotrougs truncatus (Brenske, 1886) and Amadotrogus vicinus (Mulsant, 1842) (Coca-Abia & Martín-Piera 1998). In addition, Rhizotrogus rugifrons Burmeister, 1855 is considered a new synonym of Amadotrogus vicinus. Rhizotrogus bolivari Martínez y Sáez, 1873, Amphimallon cata- launicum Báguena, 1956 and Rhizotrogus lajonquierei Baraud, 1970 are synonymized with Amadotrogus patruelis.
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