ABSTRACT Driven by the growing demand for good quality food, agriculture is changing the way of cultivation to attend consumer’s expectations. In addition to the nutritional value, the production ...system has been an important factor in the consumer’s intention to purchase products. In this scenario, this work aimed to evaluate the effects of different nutrient sources on production characteristics and nutritional quality of 'Veneranda' lettuce. The experiment was conducted in randomized block design, with seven treatments (control, bokashi, organic compost, mineral fertilizer, mineral fertilizer + organic compost, foliar biofertilizer and soil biofertilizer), and five replications. In commercial vegetative stage, lettuce was harvested and evaluated for physical, physicochemical, bioactive compounds and mineral composition. Lettuce produced with mineral and organomineral fertilizers showed the highest yield of physical characteristics, which is related to the highest nitrogen content in their leaves. The use of organomineral fertilization, organic and mineral compost and biofertilizer via leaf application presented a positive effect on lettuce cultivation, with better nutritional quality and productivity.
RESUMO Impulsionada pela crescente demanda por alimentos de boa qualidade, a agricultura está mudando a forma de cultivo para atender às expectativas dos consumidores. Além do valor nutritivo, o sistema de produção tem sido um fator importante na intenção de compra dos consumidores. Diante desse cenário, este trabalho teve como objetivo avaliar os efeitos de diferentes fontes de nutrientes nas características de produção e qualidade nutricional da alface Veneranda. O experimento foi conduzido em delineamento em blocos casualizados, com sete tratamentos (controle, bokashi, composto orgânico, fertilizante mineral, fertilizante mineral + composto orgânico, biofertilizante via foliar e biofertilizante via solo) e cinco repetições. No estádio vegetativo comercial, a alface foi colhida e avaliada quanto a aspectos físicos, físico-químicos, compostos bioativos e composição mineral. As alfaces produzidas com fertilizantes minerais e organomineral apresentaram o maior rendimento das características físicas, o que está relacionado ao maior teor de nitrogênio em suas folhas. A utilização de adubação organomineral, composto orgânico e mineral e biofertilizante via foliar apresentou efeito positivo no cultivo da alface, com melhor qualidade nutricional e produtividade.
Although mesenchymal stromal (stem) cell (MSC) administration attenuates sepsis-induced lung injury in pre-clinical models, the mechanism(s) of action and host immune system contributions to its ...therapeutic effects remain elusive. We show that treatment with MSCs decreased expression of host-derived microRNA (miR)-193b-5p and increased expression of its target gene, the tight junctional protein occludin (Ocln), in lungs from septic mice. Mutating the Ocln 3' untranslated region miR-193b-5p binding sequence impaired binding to Ocln mRNA. Inhibition of miR-193b-5p in human primary pulmonary microvascular endothelial cells prevents tumour necrosis factor (TNF)-induced decrease in Ocln gene and protein expression and loss of barrier function. MSC-conditioned media mitigated TNF-induced miR-193b-5p upregulation and Ocln downregulation
When administered
, MSC-conditioned media recapitulated the effects of MSC administration on pulmonary miR-193b-5p and Ocln expression. MiR-193b-deficient mice were resistant to pulmonary inflammation and injury induced by lipopolysaccharide (LPS) instillation. Silencing of Ocln in miR-193b-deficient mice partially recovered the susceptibility to LPS-induced lung injury.
inhibition of miR-193b-5p protected mice from endotoxin-induced lung injury. Finally, the clinical significance of these results was supported by the finding of increased miR-193b-5p expression levels in lung autopsy samples from acute respiratory distress syndrome patients who died with diffuse alveolar damage.
Amid research into the mounting social and environmental threats presented by climate change, young people’s everyday experiences and knowledges are often overlooked, despite being the generation ...that will be most affected by climate change. We present a ‘looping’ methodology, developed through collaborative work between two distinct but complementary research projects wherein young people in the Paraíba do Sul watershed, São Paulo state, Brazil shared their perspectives on (respectively) climate-related disasters and the food–water–energy nexus. The approach brings together the theoretical framings of citizen science and nexus thinking under the aegis of participatory action research, to identify points of mutual learning in relation to the knowledge, action and critique co-produced with young people. This ‘looping methodology’ enables meta-analytic insights into how participatory action research, looped with other forms of action-oriented research, can enable young people and other protagonists to articulate and act upon the complex, multi-scalar processes that characterise what it means to live in uncertain social, political and environmental times.
Abstract The objective of this work was to determine the acidity levels to obtain adequate soil chemical attributes for the maximum fruit yield of blackberry trees grown in acidic soils subjected to ...increasing limestone rates. The BRS Tupy, Brazos, Guarani, and BRS Xavante blackberry cultivars were evaluated on the Typic Hapludox (LVdf) and Rhodic Hapludox (LVd) soils, to which four rates of dolomitic limestone were applied (0, 1.5, 3.0, and 6.0 Mg ha-1). The effect of liming on soil chemical attributes, in a 2×4 factorial arrangement (soils and limestone rates), as well as leaf nutrient content and fruit yield, in a 2×4×4 factorial arrangement (soils, limestone rates, and cultivars), were evaluated. The maximum fruit yield was obtained with 3.3 Mg ha-1 dolomitic limestone. The blackberry cultivars showed a high demand for Ca, Mg, and base saturation and a low tolerance to aluminum in the soils. From 3.3 Mg ha-1 dolomitic limestone, the maximum fruit yield of blackberry trees grown in acidic soils is obtained with pH 5.6, maximum tolerated aluminum saturation of 6%, Ca and Mg concentration of 45.3 mmolc kg-1, and base saturation of 48.3%.
Resumo O objetivo deste trabalho foi determinar os níveis de acidez para obter os atributos químicos do solo adequados à máxima produção de frutos de amoreiras-pretas cultivadas em solos ácidos submetidos a doses crescentes de calcário. Foram avaliadas as cultivares de amoreira-preta BRS Tupy, Brazos, Guarani e BRS Xavante nos solos Latossolo Vermelho Distroférico (LVdf) e Latossolo Vermelho distrófico (LVd), aos quais foram aplicadas quatro doses de calcário dolomítico (0, 1,5, 3,0 e 6,0 Mg ha-1). Foram avaliados o efeito da calagem sobre os atributos químicos do solo, em arranjo fatorial 2×4 (solos e doses de calcário), bem como o teor de nutrientes foliares e a produção de frutos, em arranjo fatorial 2×4×4 (solos, doses de calcário e cultivares). A produção máxima de frutos foi obtida com 3,3 Mg ha-1 de calcário dolomítico. As cultivares de amoreira-preta apresentaram alta demanda por Ca, Mg e saturação por bases e baixa tolerância ao alumínio nos solos. A partir de 3,3 Mg ha-1 de calcário dolomítico, a produção máxima de frutos de amoras-pretas cultivadas em solos ácidos é obtida com pH 5,6, saturação máxima tolerada de alumínio de 6%, concentração de Ca e Mg de 45,3 mmolc kg-1 e saturação por bases de 48,3%.
Blackberry is grown in a wide range of soil pH, and it is known that the adequacy of soil pH is essential for plants to express their maximum nutritional efficiencies and, consequently, reach their ...maximum productive potential. Thus, the objective was to evaluate the nutritional accumulations and efficiencies for maximum fruit production. The experiment under greenhouse conditions was carried out in a randomized block design followed by a 4 × 2 × 4 factorial scheme with three repetitions. The evaluated factors were four blackberry cultivars (Tupy, Brazos, Guarani, and Xavante) grown in two types of acidic soils (Typical Hapludox TH and Rhodic Hapludox RH) to which four rates of dolomitic limestone (0, 1.5, 3.0, and 6.0 Mg ha
−1
) were applied. Fruit production and nutrient accumulation in the fruits, leaves, stems, and roots of the plants were evaluated, as well as the nutritional efficiency of each cultivar. The blackberry cultivar Tupy has been the most productive, independent of the liming rate. Blackberry cultivars have shown differences in nutrient accumulation and efficiencies for maximum fruit production, and they also have distinct nutritional requirements.
Incorporation of probiotic bacteria in food is a challenge for the industry, given the sensitivity of probiotic cultures to process conditions. The objective of this study was to coat papaya slices ...with probiotic filmogenic solution to obtain dehydrated probiotic papaya slices. The probiotic filmogenic solution was applied as an edible coating on papaya slices before drying operation at temperatures of 50, 60, and 70°C. The Page model showed the best fit for all kinetics (R² > 0.9988). The use of filmogenic solution to incorporate the probiotic in papaya slices showed satisfactory results, with a reduction of 1.30 LogCFU g−1 at the end of drying at 70°C, reduced shrinkage, resulting in greater conservation of their structure and compounds phenolics. The thermodynamic properties indicate that the coated papaya slices need more energy to start the diffusion process and have a darker color and firmer texture profile.
Novelty impact statement
The difference of this study is the evaluation of the potential use of a filmogenic solution based on gelatin and inulin in the incorporation of probiotic in dried fruit. In addition, the use of edible coating as a pretreatment for drying fruits gives interesting technological characteristics to the dried product, increasing its nutritional value.
Sepsis is a complicated multi-system disorder characterized by a dysregulated host response to infection. Despite substantial progress in the understanding of mechanisms of sepsis, translation of ...these advances into clinically effective therapies remains challenging. Mesenchymal Stromal Cells (MSCs) possess immunomodulatory properties that have shown therapeutic promise in preclinical models of sepsis. The therapeutic effects of MSCs may vary depending on the source and type of these cells. In this comparative study, the gene expression pattern and surface markers of bone marrow-derived MSCs (BM-MSCs) and umbilical cord-derived MSCs (UC-MSCs) as well as their therapeutic effects in a clinically relevant mouse model of polymicrobial sepsis, cecal ligation and puncture (CLP), were investigated. The results showed remarkable differences in gene expression profile, surface markers and therapeutic potency in terms of enhancing survival and pro/anti-inflammatory responses between the two MSC types. BM-MSCs improved survival concomitant with an enhanced systemic bacterial clearance and improved inflammatory profile post CLP surgery. Despite some improvement in the inflammatory profile of the septic animals, treatment with UC-MSCs did not enhance survival or bacterial clearance. Overall, the beneficial therapeutic effects of BM-MSCs over UC-MSCs may likely be attributed to their pro-inflammatory function, and to some extent anti-inflammatory features, reflected in their gene expression pattern enhancing macrophage polarization to M1/M2 phenotypes resulting in a balanced pro- and anti-inflammatory response against polymicrobial sepsis.
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•Mesenchymal stromal cells (MSCs) may be a therapeutic option for sepsis.•Bone marrow (BM) vs. umbilical cord (UC) -MSCs induce different M1/M2 gene profiles.•In a CLP model of sepsis, only BM-MSCs improved survival and bacterial clearance.
Critical illnesses including sepsis, acute respiratory distress syndromes, ischemic cardiovascular disorders and acute organ injuries are associated with high mortality, morbidity as well as ...significant health care system expenses. While these diverse conditions require different specific therapeutic approaches, mesenchymal stem/stromal cell (MSCs) are multipotent cells capable of self-renewal, tri-lineage differentiation with a broad range regenerative and immunomodulatory activities, making them attractive for the treatment of critical illness. The therapeutic effects of MSCs have been extensively investigated in several pre-clinical models of critical illness as well as in phase I and II clinical cell therapy trials with mixed results. Whilst these studies have demonstrated the therapeutic potential for MSC therapy in critical illness, optimization for clinical use is an ongoing challenge. MSCs can be readily genetically modified by application of different techniques and tools leading to overexpress or inhibit genes related to their immunomodulatory or regenerative functions. Here we will review recent approaches designed to enhance the therapeutic potential of MSCs with an emphasis on the technology used to generate genetically modified cells, target genes, target diseases and the implication of genetically modified MSCs in cell therapy for critical illness.
Abstract Objective To evaluate whether the quadrivalent human papillomavirus (HPV) (types 6, 11, 16, and 18) vaccine influences the clinical course of juvenile-onset recurrent respiratory ...papillomatosis (RRP) when administered to a group of patients with this condition. Methods Uncontrolled intervention study of patients with juvenile-onset RRP examined at the Pediatric Otorhinolaryngology Clinic, Federal University of São Paulo, where nine patients between the ages of nine and 17 received three doses of the prophylactic quadrivalent HPV vaccine (Gardasil® ) and were followed for one year. Disease staging, intervals between relapses, intervals between surgeries, and the number of surgeries during the year prior to vaccination and during the first year after vaccination were compared. Results Eight patients were infected with HPV-6 and one with HPV-11. There were no statistically significant differences in the clinical scores ( p = 0.083), anatomical scores ( p = 0.257), intervals between relapses ( p = 0.062), intervals between surgeries ( p = 0.357), or the numbers of surgeries ( p = 0.180) when the years before and after vaccination were compared. All patients had relapses following vaccination. Conclusion Patients with juvenile-onset RRP experienced a similar clinical course in the year after versus the year before vaccination with Gardasil®.
Circular single stranded DNA viruses (CRESS DNA) encoding a homologous replication-associated protein (REP) have been identified in most of eukaryotic groups. It is not clear yet the role in human ...diseases or details of the life cycle of these viruses. Recently, much interest has been raised in the evolutionary history of CRESS DNA owing to the increasing number of new sequences obtained by Next-Generation Sequencing (NGS) in distinct host species. In this study we describe two full-length CRESS DNA genomes obtained of two newly diagnosed HIV patients from São Paulo State, Brazil. The initial BLASTx search indicated that both sequences (named SP-FFB/2020 and SP-MJMS/2020) are highly similar (98%) to a previous CRESS DNA sequence detected in human fecal sample from Peru in 2016 and designated as pecovirus (Peruvian stool-associated circo-like virus). This study reported for the first time the Human feces pecovirus in the feces of two newly diagnosed HIV patients in Brazil. Our comparative analysis showed that although pecoviruses in South America share an identical genome structure they diverge and form distinct clades. Thus, we suggest the circulation of different species of pecoviruses in Latin America. Nevertheless, further studies must be done to examine the pathogenicity of this virus.
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