•Protocatechuic acid (PA) was successfully lipophilized using alcohols from C1 to C18.•Lipophilization could improve PA antioxidant capacity in heterogeneous media.•Antioxidant activity is related to ...compound polarity and method of evaluation.•Protocatechuates could be considered as potential preservatives in food.•An innovative way to add-value to phenolic-rich vegetal extracts is proposed.
Hibiscus sabdariffa L. is a worldwide consumed plant, principally after infusion of its dried sepals and calyces, which are usually discarded. Nevertheless, they represent a potential source of natural bioactive compounds, e.g. polyphenols, which could add value to this under-exploited plant. Protocatechuic acid (PA) was chosen as a model of the phenolic acids that can be extracted from H. sabdariffa. In order to modify PA hydrophilic character, which limits its use in lipid-rich food products, PA was esterified to C1–C18 alcohols, and the impact of lipophilization on its antioxidant activity was evaluated in both, an homogeneous (DPPH and ORAC methods) and an heterogeneous (CAT method) system. Results herein obtained showed that, depending on the grafted alkyl chain length, lipophilization could positively affect the antioxidant activity of PA in heterogeneous media; therefore, support its use as an innovative way to synthesize molecules with an improved antioxidant capacity and potential to be used as multifunctional preservatives in food.
Summary
Sweet pickled mango named Ma‐Muang Bao Chae‐Im is a traditional preserved mango from Hat Yai, Thailand. This study investigated (I) volatile and non‐volatile compound profiles of commercial ...Ma‐Muang Bao Chae‐Im and (II) their relationship to consumer preference. Untargeted metabolomics profiling was performed by gas chromatography‐mass quadrupole‐time of flight analysis. There were 117 volatile and 44 non‐volatile compounds annotated in six commercial brands of Ma‐Muang Bao Chae‐Im. Furthermore, 46 volatile and 19 non‐volatile compounds’ discriminant markers were found by Partial least square discriminant analysis. Among those markers, sorbic and benzoic acid were observed in several brands; moreover, the combination of both compounds altered the volatile profile, especially the ester group. Partial least square regression revealed that overall consumer liking is correlated to 1‐heptanol; 1‐octanol; acetoin; acetic acid, 2‐phenylethyl ester; D‐manitol; terpenes and terpenoids, while firmness to sucrose and L‐(‐)‐sorbofuranose. On the other hand, most ester compounds were not related to consumer preference.
The workflow of volatile and non‐volatile compound profiles of commercial sweet pickled mango and its correlation with consumer preference.
Summary
The chemical characteristics and volatile compounds profiles in distinct anatomical sites of a farm‐raised hybrid catfish (Clarias macrocephalus × Clarias gariepinus) were investigated. Total ...haem proteins, trimethylamine‐nitrogen, TBARS, n‐9 oleic acid, n‐6 linoleic acid and total concentration of all volatile compounds were all higher in the lateral line with the maximum redness index (p < 0.05). Dorsal muscle lipid contained more eicosapentaenoic acid and docosahexaenoic acid than the other two muscles (p < 0.05). Ventral muscle exhibited the highest fat content (p < 0.05) and was the only muscle discovered to have n‐3 linolenic acid. Geosmin and 2‐methylisoborneol (2‐MIB) were found in all three muscles. Despite the fact that 2‐MIB and geosmin are lipophilic compounds, their distribution was unaffected by fat content, with the highest levels in the lateral line (p < 0.05). Odour activity values (OAV) and sensory analyses revealed that not only 2‐MIB and geosmin but also (E)‐2‐nonenal and 1‐octen‐3‐ol, intensified the earthy and muddy odour in catfish muscles. The total off‐odour of catfish muscles was influenced by n‐9 oleic acid and n‐6 linoleic acid, according to the correlation analysis. The off‐odour compounds had a substantial negative connection with total n‐3 PUFA, as well as the irrelevance with fat content, according to this study.
Chemical indices and volatile compounds of fresh hybrid catfish were affected by muscle location. The results can be used to develop post‐mortem handling and processing criteria to address off‐flavour issues and increase the market value of farmed hybrid catfish.
Summary
The antioxidant activity and stability of endogenous peptides isolated from farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) muscle (EPC) were characterised. EPC contained ...69 peptides with 8–24 amino acid residues, varying hydrophobic amino acid (HBA) content and distinct sequences. Among the top five, peptides discovered in EPC were ARHSYGMLYCSCPPND, DTQAARKSDDDD, AEFPCGDRRC, AAVTEELFFAGL and LILQRRKFLRMKREKYGFIYKTHL. Histidine (16.33%) and tryptophan (15.66%) were the most common amino acids found in EPC. EPC demonstrated concentration‐dependent free radical (DPPH•/ABTS•+) and hydroxyl radical (OH•) scavenging activities, as well as metal chelating ability. The effect of pH, heating temperature and in vitro digestion on EPC's DPPH• scavenging activity was studied. Lowering the pH and increasing the heating temperature to 90 °C increased the DPPH• inhibitory activity. However, after in vitro digestion, around 44% of DPPH• inhibition was reduced. The findings confirmed that farm‐raised hybrid catfish muscle contained endogenous peptides with antioxidant properties.
Farm‐raised hybrid catfish muscle contained endogenous peptides with antioxidant properties.
Cannabidiol (CBD) is a natural phytochemical agent and one of the most abundant found in
. It is known to exhibit pharmacological properties on various condition such as relieving-inflammation, pain, ...epilepsy, and anxiety effect. There has been an increasing trend globally in the use of CBD as a supplement in pets. Consequently, there are various CBD products being marketed that are specifically available for pets. Veterinarians and pet owners are concerned that following ingestion, different CBD formulations may result in a CBD level circulating in the blood that may affect the safe use and efficacy of CBD in pets. Several pharmacokinetics studies in animals have been mainly conducted with an oily form of CBD. To date, there is a lack of data regarding direct comparisons in animals among the CBD plasma kinetic profiles from an oral administration of the various preparation forms. Therefore, the current study evaluated and compared the plasma CBD levels from a single oral administration using four different CBD preparations-liquid (an oil-based form, a nanoemulsion form, or a water-soluble form) or a semi-solid form (as CBD mixed in a treat) in dogs. In total, 32 healthy, crossbreed dogs were randomly assigned into 4 groups and treated according to a 1-period, 4-treatment parallel-design. The three liquid forms were dosed at 5 mg/kg body weight, while the single semi-solid form was given at 50 mg/treat/dog. The results showed that the CBD plasma profile from the administration of a water-soluble form was comparable to that of the oil-based group. The nanoemulsion-based form tended to be rapidly absorbed and reached its peak sooner than the others. However, the CBD in all preparations reached the maximum plasma concentration within 3 h post-dose, with an average range of 92-314 μg/L. There were significant differences among certain parameters between the liquid and semi-solid forms. This was the first study to provide pharmacokinetics data regarding CBD in water soluble, nanoemulsion-based, and semi-solid forms for dogs as companion animals. The current data should facilitate the scrutiny of CBD plasma profiles based on different formulations via an oral route in dogs.
The tail tendons of skipjack tuna (
), a by-product from the meat-separation process in canned-tuna production, was used as an alternative source of collagen extraction. The acid-solubilized ...collagens using vinegar (VTC) and acetic-acid (ATC) extraction and pepsin-solubilized collagen (APTC) were extracted from tuna-tail tendon. The physiochemical properties and characteristics of those collagens were investigated. The obtained yield of VTC, ATC, and APTC were 7.88 ± 0.41, 8.67 ± 0.35, and 12.04 ± 0.07%, respectively. The determination of protein-collagen solubility, the effect of pH and NaCl on collagen solubility, Fourier-transform infrared spectroscopy (FTIR) spectrum, and microstructure of the collagen-fibril surface using a scanning electron microscope (SEM) were done. The protein solubility of VTC, ATC, and APTC were 0.44 ± 0.03, 0.52 ± 0.07, and 0.67 ± 0.12 mg protein/mg collagen. The solubility of collagen decreased with increasing of NaCl content. These three collagens were good solubility at low pH with the highest solubility at pH 5. The FTIR spectrum showed absorbance of Amide A, Amide B, Amide I, Amide II, and Amide III groups as 3286-3293 cm
, 2853-2922 cm
, 1634-1646 cm
, 1543-1544 cm
, and 1236-1237 cm
, respectively. The SEM analysis indicated a microstructure of collagen surface as folding of fibril with small pore.
Studies have shown that Flavivirus infection remodels the host cell to favour viral replication. In particular, the host cell lipid profile is altered, and it has been proposed that this process ...alters membrane fluidity to allow wrapping of the outer structural proteins around the viral nucleocapsid. We investigated whether expression of the Zika virus (ZIKV) and dengue virus (DENV) protease induced alterations in the cellular lipid profile, and subsequently whether co-expression of these proteases with VLP constructs was able to improve VLP yield. Our results showed that both ZIKV and DENV proteases induced alterations in the lipid profile, but that both active and inactive proteases induced many of the same changes. Neither co-transfection of protease and VLP constructs nor bicistronic vectors allowing expression of both protease and VLP separated by a cell cleavable linker improved VLP yield, and indeed many of the constructs showed significantly reduced VLP production. Further work in developing improved VLP expression platforms is required.
This study sought to identify the mechanism underlying the response to chitosan at the posttranslational level. Khao Dawk Mali 105 seeds were soaked in 40 mg l
−1
of chitosan, and leaves of 2- and ...4-week-old seedlings were sprayed with chitosan before starting osmotic stress conditions. Chitosan induced resistance to osmotic stress by enhancing shoot fresh and dry weights and maintained increased photosynthetic pigments. Leaf phosphoproteomes were examined using gel-free LC-MS/MS. Of the 60 phosphoproteins showed a significant difference in protein expressions under osmotically-stressed plants treated with chitosan. More than 40% of the phosphoproteins involved in signaling pathways, including OsCML12 calmodulin-related calcium sensor protein, ubiquitin carboxyl-terminal hydrolase 15, U-box domain-containing protein 45, HEAT repeat family protein, BRCA1 C terminus domain-containing protein, pectinesterase, protein kinase domain-containing protein, and receptor-like protein kinase. Chitosan enhanced rice seedling growth and drought resistance via multiple complex networks, including metabolism, transport, transcription, and signaling under osmotic stress.
Oil blending is a method that may improve the nutritional profile and stability of frying oil. Tropical vegetable oils, including rice bran oil, coconut oil, and palm oil were blended at ratios of 20 ...: 20 : 60, 25 : 25 : 50, 30 : 30 : 40, and 35 : 35 : 30 (v/v/v), respectively, and tested for their performance in deep frying French fries at 180ºC for 8 h. The nutritional content of the blended oils increased with the rice bran oil and coconut oil ratio, including polyunsaturated fatty acids, α-tocopherol and γ-oryzanol. The physicochemical property changes, including color, viscosity, fatty acid profile, total polar compounds, free fatty acid, peroxide value, and the thiobarbituric acid reactive substances value of the blended oils were monitored during frying. The lightness of the oil blends was higher than those of palm oil after frying. However, the higher ratio of rice bran oil and coconut oil resulted in a higher increase in viscosity during frying. The oxidative stabilities of the oil blends were better than that of palm oil. Additionally, the sensory characteristics of the fries prepared in these oil blends were evaluated using a 9-point hedonic scale. There was no significant difference in sensory attributes of the fries produced using different oils. The oil blended at a ratio of 30 : 30 : 40 shows the greatest performance as a deep frying media compared to the other blended oils that were tested.
This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the ...CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress.
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