Due to the wet and dynamic environment of the oral cavity, the healing of intraoral wounds, such as tooth extraction wounds, requires stable and firm wound dressings. In clinical practice, cotton ...balls and gauzes, sponge plugs, or sutures are used to treat extraction wounds, but none of these means can continuously isolate the wound from the intraoral environment and facilitate ideal healing conditions. Herein, inspired by the natural extracellular matrix, a family of wound dressings is developed for intraoral wound repair. Infiltrating a ductile long‐chain hydrogel network into a prefabricated, sturdy macromolecular meshwork and in situ crosslinking endowed the composite hydrogel with controllable swelling behaviors and robust mechanical properties. The macromolecular meshwork functioned as the backbone to support the composite and restricts the swelling of the long‐chain hydrogel network. In vitro tests verified that this wound dressing can provide durable protection for intraoral wounds against complex irritations. Furthermore, accelerated wound healing occurred when the wound dressing is applied in vivo on a canine tooth extraction model, due to the effective reduction of acute inflammation. These results suggest that this family of bioinspired hydrogels has great potential for application as intraoral wound dressing.
An extracellular matrix‐mimicking hydrogel wound dressing is designed as tooth extraction adhesive wound dressing for intraoral application. The hydrogel exhibits excellent anti‐swelling efficiency, along with consistent mechanical and adhesive robustness in aqueous environments. In vitro and in vivo tooth extraction models demonstrate the stability of hydrogels as physical and antibacterial barriers for intraoral wounds, leading to continuous protection and effective healing acceleration.
Abstract
Nanoparticles-based glues have recently been shown with substantial potential for hydrogel adhesion. Nevertheless, the transformative advance in hydrogel-based application places great ...challenges on the rapidity, robustness, and universality of achieving hydrogel adhesion, which are rarely accommodated by existing nanoparticles-based glues. Herein, we design a type of nanohesives based on the modulation of hydrogel mechanics and the surface chemical activation of nanoparticles. The nanohesives can form robust hydrogel adhesion in seconds, to the surface of arbitrary engineering solids and biological tissues without any surface pre-treatments. A representative application of hydrogel machine demonstrates the tough and compliant adhesion between dynamic tissues and sensors via nanohesives, guaranteeing accurate and stable blood flow monitoring in vivo. Combined with their biocompatibility and inherent antimicrobial properties, the nanohesives provide a promising strategy in the field of hydrogel based engineering.
Background and Aim
Chinese herbal medicine (CHM), as well as Western medicine (WM), is an important cause of drug‐induced liver injury (DILI). However, the differences between CHM and WM as agents ...implicated in liver injury have rarely been reported.
Methods
Overall, 1985 (2.05%) DILI cases were retrospectively collected from the 96 857 patients hospitalized because of liver dysfunction in the 302 Military Hospital between January 2009 and January 2014.
Results
In all the enrolled patients with DILI, CHM was implicated in 563 cases (28.4%), while 870 cases (43.8%) were caused by WM and the remaining patients (27.8%) by the combination of WM and CHM. Polygonum multiflorum was the major implicated CHM. Compared with WM, the cases caused by CHM showed more female (51 vs 71%, P < 0.001) and positive rechallenge (6.1 vs 8.9%, P = 0.046), a much greater proportion of hepatocellular injury (62.2 vs 88.5%, P < 0.001), and a higher mortality (2.8 vs 4.8%, P = 0.042); however, no differences in the rates of chronic DILI and ALF were found (12.9 vs 12.4%, P = 0.807; 7.6 vs 7.6%, P = 0.971). Based on Roussel Uclaf Causality Assessment Method, 75.6% of cases caused by CHM were classified as probable and only 16.6% as highly probable, significantly different from WM (38.4 and 60.3%, all P < 0.001).
Conclusions
The causal relationship between CHM and liver injury is much complex, and the clinical characteristics of DILI caused by CHM differ from those caused by WM.
Background. Aberrant DNA methylation patterns are of increasing interest in the study of psoriasis mechanisms. This study aims to screen potential diagnostic indicators affected by DNA methylation ...for psoriasis based on bioinformatics using multiple machine learning algorithms and to preliminarily explore its molecular mechanisms. Methods. GSE13355, GSE14905, and GSE73894 were collected from the gene expression omnibus (GEO) database. Differentially expressed genes (DEGs) and differentially methylated region- (DMR-) genes between psoriasis and control samples were combined to obtain differentially expressed methylated genes. Subsequently, a protein-protein interaction (PPI) network was established to analyze the interaction between differentially expressed methylated genes. Moreover, the hub genes of psoriasis were screened by the least absolute shrinkage and selection operator (LASSO), Random Forest (RF), and Support Vector Machine (SVM), which were further performed single-gene gene set enrichment analysis (GSEA) to clarify the pathogenesis of psoriasis. The druggable genes were predicted using DGIdb. Finally, the expressions of hub genes in psoriasis lesions and healthy controls were detected by immunohistochemistry (IHC) and quantitative real-time PCR (RT-qPCR). Results. In this study, a total of 767 DEGs and 896 DMR-genes were obtained. Functional enrichment showed that they were significantly associated with skin development, skin barrier function, immune/inflammatory response, and cell cycle. The combined transcriptomic and DNA methylation data resulted in 33 differentially expressed methylated genes, of which GJB2 was the final identified hub gene for psoriasis, with robust diagnostic power. IHC and RT-qPCR showed that GJB2 was significantly higher in psoriasis samples than those in healthy controls. Additionally, GJB2 may be involved in the development and progression of psoriasis by disrupting the body’s immune system, mediating the cell cycle, and destroying the skin barrier, in addition to possibly inducing diseases related to the skeletal aspects of psoriasis. Moreover, OCTANOL and CARBENOXOLONE were identified as promising compounds through the DGIdb database. Conclusion. The abnormal expression of GJB2 might play a critical role in psoriasis development and progression. The genes identified in our study might serve as a diagnostic indicator and therapeutic target in psoriasis.
Background
Membranous aplasia cutis congenita (MACC) presents at birth characterized by oval epidermis defect. Skin lesions with MACC have various clinic manifestations. In recent years, the ...usefulness of trichoscopy (scalp dermoscopy) has been reported for hair loss diseases. However, the dermoscopic features of MACC were mostly reported by case reports.
Objectives
To summarized the obvious dermoscopic characteristics of MACC.
Materials & Methods
These 56 cases met the clinical diagnostic criteria for MACC without forceps delivery complications or other birth injuries. To find the dermoscopic characteristics of MACC by summarizing 56 infants' dermoscopic pictures.
Results
The dermoscopic manifestation of MACC are characterized by hair follicle openings and hair deficiency in the center of skin lesions, translucent epidermis, hair root and hair bulb arranged along the margins of skin lesion.
Conclusion
The typical dermoscopic characteristics of MACC could help clinicians to early diagnose and differential diagnosis.
Abstract
Background
Treating complicated urinary tract infections (cUTIs) caused by ESBL-producing Enterobacterales represents a significant clinical challenge. The present study was thus developed ...to explore the relative efficacy of β-lactam/β-lactamase inhibitors (BLBLIs) and carbapenems for the treatment of hospitalized patients suffering from cUTIs caused by BLBLI-susceptible ceftriaxone-non-susceptible Enterobacterales.
Methods
Data from 557 patients from four Chinese teaching hospitals diagnosed with cUTIs caused by ceftriaxone-non-susceptible Enterobacterales from January 2017 to May 2022 were retrospectively assessed.
Result
The 30 day rate of treatment failure, defined by unresolved symptoms or mortality, was 10.4% (58/557). Independent predictors of 30 day treatment failure included immunocompromised status, bacteraemia, septic shock, lack of infection source control and appropriate empirical treatment. When data were controlled for potential confounding variables, BLBLI treatment exhibited a comparable risk of 14 day (OR 1.61, 95% CI 0.86–3.00, P = 0.133) and 30 day treatment failure (OR 1.45, 95% CI 0.66–3.15, P = 0.354) relative to carbapenem treatment for the overall cohort of patients. In contrast, BLBLI treatment in immunocompromised patients was associated with an elevated risk of both 14 day (OR 3.18, 95% CI 1.43–7.10, P = 0.005) and 30 day treatment failure (OR 3.06, 95% CI 1.07–8.80, P = 0.038) relative to carbapenem treatment.
Conclusions
These results suggested that carbapenem treatment may be superior to BLBLI treatment for immunocompromised patients suffering from cUTIs caused by ceftriaxone-non-susceptible Enterobacterales species. However, these results will need to be validated in appropriately constructed randomized controlled trials to ensure appropriate patient treatment.
Guided bone regeneration (GBR) membranes have been commonly used for bone defect repair in clinical medicine. However, the existing GBR membranes are often limited by weak mechanical strength, poor ...antibacterial effect, overquick degradation or non-degradation, etc. Here, inspired by natural nacre and the most commonly used GBR membrane (Bio-Gide), we report a new kind of GBR membrane fabricated by combining evaporation-induced self-assembly with a subsequent ice-templating procedure. Similar to the Bio-Gide membrane, it also consists of a bilayer structure including a compact nacre-like layer and a porous layer. Furthermore, it exhibits high mechanical properties and other functions not achieved by the Bio-Gide membrane. The obtained high mechanical properties and multiple functions, including effective bacteriostasis, appropriate degradation rate, and biocompatibility, are superior to those of previously reported GBR membranes. This multifunctional bilayer membrane, fabricated via a simple and straightforward method, may be an appropriate candidate as a bioactive GBR membrane for clinical application.
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•Evaporation-induced self-assembly and ice-templating technique are combined•A unique bilayer structure is designed to fulfill functional requirement•Nacre-inspired structure endows the GBR membrane with high strength and toughness•Most biological functions for an ideal GBR membrane were achieved
Treatment of bone defects by applying a barrier membrane, namely a guided bone regeneration (GBR) membrane, is commonly used in clinical medicine. In contrast to commercially available GBR membranes such as collagen membrane or titanium-reinforced PTFE membrane, which exhibit the problems of weak mechanical strength, poor antibacterial effect, overquick degradation or non-degradation, etc., we demonstrated a multifunctional bilayer nanocomposite GBR membrane fabricated by combining nacre-inspired self-assembly with an ice-templating self-assembly technique. The compact nacre-like layer provides a barrier function while ensuring high strength and toughness of the GBR membrane. The porous layer is designed for promoting osteoblast adhesion. In addition, effective antimicrobial activity, appropriate degradation rate, and favorable biocompatibility are fulfilled, rendering this multifunctional GBR membrane as an appropriate candidate for clinical application.
The guided bone regeneration (GBR) technique, based on applying a physical barrier membrane, is one of the most effective approaches to achieve osteogenesis. Inspired by biological functions for an ideal GBR membrane, the bilayer multifunctional GBR membrane designed in this work exhibits superior mechanical properties and relevant biological functions, which is distinct from previously reported GBR membranes and may be an appropriate candidate as a bioactive GBR membrane for clinical application. This would boost the substantial progress of biomedical engineering.
Salacia menglaensis J.Y. Shen, L.C. Yan & S. Landrein (Celastraceae), a new species from Huidu, Mengla, Yunnan, China, is described and illustrated. It is similar to S. korthalsiana, S. glaucifolia ...and S. polysperma, but differs from them by the fruit size (2–3 × 2–3 cm), with only 1–2 mature seeds and by the non-glaucous abaxial leaf surface. We also provide a conservation assessment for this rare vine species.
The present study was conducted to investigate the relationship between the anti-stress and hepato-protective effects of Schisandra Lignans Extract (SLE) on stress-induced liver damage. Seven weeks ...old male mice were fixed in a restraint tube for 18 h to induce liver damage. SLE was orally administered to animals for 5 days at dosages of 100 and 200 mg/kg/day before exposed to restraint stress. Oral administration of SLE significantly reduced restraint-induced liver damage in experimental animal. SLE was further found to significantly alleviate the provocation of corticosterone in stressed mice. SLE also significantly decreased oxidative damage and increased anti-oxidative capability of liver cells by preventing the over production and accumulation of free radicals. In conclusion, the protective effects of SLE on stress-induced liver damage were confirmed, and the correlation between hepatoprotective and anti-stress effects of schisandra lignans was possible related to its alleviation on the malignant effects of stressors for bio-homeostasis, such as balance of oxidation and reduction in cells.
Abstract Many studies have reported the association between the FASLG –844T/C polymorphism and cancer risk, but the data are remaining controversial. A pooled analysis was performed to assess this ...relationship comprehensively. Medline, PubMed, Embase and Web of Science were searched, and data were extracted and cross-checked independently by three authors. A total of 18 published studies including 22389 subjects were involved in this analysis. Overall, the –844C allele was associated with a significantly increased cancer risk (for CC versus TT: OR = 1.23, 95% confidence interval (CI) = 1.04–1.45; for CC + TC versus TT: OR = 1.15, 95% CI = 1.01–1.30; for CC versus TT + TC: OR = 1.20, 95% CI = 1.05–1.38). In the subgroup analysis by ethnicity, significantly elevated risks were found among Asians (for CC versus TT: OR = 1.61, 95% CI = 1.37–1.89; for CC + TC versus TT: OR = 1.36, 95% CI = 1.16–1.60; for CC versus TT + TC: OR = 1.44, 95% CI = 1.22–1.70). In the subgroup analysis by study design, significantly increased risks were found among population-based case-control studies (for CC versus TT: OR = 1.40, 95% CI = 1.06–1.84; for CC + TC versus TT: OR = 1.25, 95% CI = 1.01–1.55; for CC versus TT + TC: OR = 1.31, 95% CI = 1.06–1.61). These findings indicate that the FASLG –844C allele is emerging as a low-penetrant cancer susceptibility allele for cancer development. However, more comprehensive understanding of the association would certainly have an immense prospect in the promising field of individualised preventive care.
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