Cardiovascular diseases are the leading cause of death worldwide, and arterial hypertension is a recognized cardiovascular risk factor that is responsible for high morbidity and mortality. Arterial ...hypertension is the result of an inflammatory process that results in the remodeling and thickening of the vascular walls, which is associated with an immunological response. Previous studies have attempted to demonstrate the relationship between oral disease, inflammation, and the development of systemic diseases. Currently, the existence of an association between periodontitis and hypertension is a controversial issue because the underlying pathophysiological processes and inflammatory mechanisms common to both diseases are unknown. This is due to the fact that periodontitis is a chronic inflammatory disease that affects the interface of teeth and surrounding tissues. However, the most likely explanation for understanding this association is related to low-grade chronic inflammation. An initial path in the study of the relationship between the mentioned pathologies is the possibility of an epigenetic influence, mediated by noncoding RNAs as microRNAs. Thus, in the present review we describe the role of microRNAs related to arterial hypertension and/or periodontitis. In addition, we identified 13 common microRNAs between periodontitis and hypertension. According to the predictions of the DIANA-mirPath program, they can regulate genes involved in 52 signaling pathways.
Dental caries corresponds to an ecological and non-contagious, dynamic and chronic disease of multifactorial origin; currently there is evidence of how genetic factors could be included as ...predisposing agents to suffer it, however this evidence is diverse and incipient. a cross-sectional study was p erformed to investigate the possible associations of DSPP (rs36094464), RUNX2 (rs566712) and KLK4 (rs198968) polymorphisms in early childhood caries. Saliva samples of children (2-11years old) were collected and genotyped for DSPP (rs36094464), RUNX2 (rs566712) and KLK4 (rs198968) polymorphisms. Through the ceft index their caries history was determined and the gene variants were students through molecular biology techniques. polymorphisms of the DSSP (rs36094464) and RUNX2 (rs566712) are associated and contribute to the susceptibility of dental caries disease in early childhood, as they are related to their history of caries. KLK4 (rs198968) polymorphisms are not associated. In conclusions, the studied polymorphisms on DSSP and RUNX2 genes are associated with changes in the tooth microarchitecture, favoring the appearance of microlesions that would contribute to dental caries disease susceptibility in early childhood. Also, no association was found for the studied polymorphism of the KLK4 gene with dental caries disease susceptibility.
Cisplatin (cis-diamminedichloroplatinum (II), DDP) is an antineoplastic agent widely used in the treatment of solid tumors because of its extensive cytotoxic activity. However, the main limiting side ...effect of DDP use is nephrotoxicity, a rapid deterioration in kidney function due to toxic chemicals. Several studies have shown that epigenetic processes are involved in DDP-induced nephrotoxicity. Noncoding RNAs (ncRNAs), a class of epigenetic processes, are molecules that regulate gene expression under physiological and pathological conditions. MicroRNAs (miRNAs) are the most characterized class of ncRNAs and are engaged in many cellular processes. In this review, we describe how different miRNAs regulate some pathways leading to cell death by apoptosis, specifically the intrinsic apoptosis pathway. Accordingly, many classes of natural products have been tested for their ability to prevent DDP-induced apoptosis. The study of epigenetic regulation for underlying cell death is still being studied, which will allow new strategies for the diagnosis and therapy of this unwanted disease, which is presented as a side effect of antineoplastic treatment.
Statins are currently the treatment of choice for hypercholesterolemia. However, wide interindividual variability has been observed in the response to treatment. Recent studies have reported the role ...of lncRNAs in the metabolism of lipids; nevertheless, there are few studies to date that show their role in the response to treatment with statins. Thus, the aim of this study was to assess the levels of expression of three lncRNAs (RP1-13D10.2; MANTIS; lncHR1) associated with genes involved in cholesterol homeostasis in leukocyte cells of hypercholesterolemic patients after treatment with atorvastatin and compare them with levels in subjects with normal cholesterol levels. A secondary aim was to assess the levels of expression in monocytic THP-1 cells differentiated to macrophages. The study included 20 subjects with normal cholesterol (NC) levels and 20 individuals with hypercholesterolemia (HC). The HC patients were treated with atorvastatin (20 mg/day/4 weeks). THP-1 cells were differentiated to macrophages with PMA and treated with different doses of atorvastatin for 24 h. Expression of lncRNAs was determined by RT-qPCR. The lncRNAs RP1-13D10.2 (
< 0.0001), MANTIS (
= 0.0013) and lncHR1 (
< 0.0001) presented increased expression in HC subjects compared with NC subjects. Furthermore, atorvastatin had a negative regulatory effect on the expression of lncHR1 (
< 0.0001) in HC subjects after treatment. In vitro, all the lncRNAs showed significant differences in expression after atorvastatin treatment. Our findings show that the lncRNAs tested present differential expression in HC patients and play a role in the variability reported in the response to atorvastatin treatment. Further research is needed to clarify the biological impact of these lncRNAs on cholesterol homeostasis and treatment with statins.
SANHUEZA, J.; BUSTOS, L.; RODRIGUEZ, N.; BORIE-ECHEVERRIA, E. & SALINAS, P. Polymorphisms in DSSP (rs36094464) and RUNX2 (rs566712) genes contribute to the susceptibility of dental caries in ...childhood. Int. J. Morphol., 39(3):802-808, 2021. Dental caries corresponds to an ecological and non-contagious, dynamic and chronic disease of multifactorial origin; currently there is evidence of how genetic factors could be included as predisposing agents to suffer it, however this evidence is diverse and incipient. a cross-sectional study was p erformed to investigate the possible associations of DSPP (rs36094464), RUNX2 (rs566712) and KLK4 (rs198968) polymorphisms in early childhood caries. Saliva samples of children (2-11years old) were collected and genotyped for DSPP (rs36094464), RUNX2 (rs566712) and KLK4 (rs198968) polymorphisms. Through the ceft index their caries history was determined and the gene variants were students through molecular biology techniques. polymorphisms of the DSSP (rs36094464) and RUNX2 (rs566712) are associated and contribute to the susceptibility of dental caries disease in early childhood, as they are related to their history of caries. KLK4 (rs198968) polymorphisms are not associated. In conclusions, the studied polymorphisms on DSSP and RUNX2 genes are associated with changes in the tooth microarchitecture, favoring the appearance of microlesions that would contribute to dental caries disease susceptibility in early childhood. Also, no association was found for the studied polymorphism of the KLK4 gene with dental caries disease susceptibility. KEY WORDS: Caries; Childhood; Polymorphisms; DSPP; RUNX2
Fundamento: la diabetes mellitus constituye una de las enfermedades crónicas más frecuentes en la población mundial, provoca daños en varios órganos y la cavidad bucal no está exenta de ella. ...Objetivo: diseñar una estrategia de intervención educativa dirigida a modificar los conocimientos sobre salud bucal en pacientes diabéticos. Métodos: se realizó una investigación observacional, de intervención longitudinal y prospectiva con un universo de 27 pacientes diabéticos, pertenecientes al Consultorio Médico número 27 que fueron atendidos en la Clínica Estomatológica Ormani Arenado Llonch en Pinar del Río, durante el año 2016, se utilizaron métodos teóricos, empíricos y estadísticos. Resultados: se diseñó y aplicó una estrategia de intervención educativa sobre salud bucal que incluyó cuatro acciones estratégicas específicas que fue validada de manera teórica por criterio de expertos, a través del Método Delphi. Conclusiones: el nivel de conocimientos sobre salud bucal de los pacientes diabéticos que en su mayoría fueron evaluados como mal antes del desarrollo de la estrategia y posterior a ello, se logró modificar de manera significativa una evaluación de bien. DeCS: SALUD BUCAL; DIABETES MELLITUS; HIGIENE BUCAL/educación; PROMOCIÓN DE LA SALUD; ENSAYO CLÍNICO.
Introducción: un estado nutricional deteriorado es un factor de riesgo importante de morbilidad y mortalidad en los adultos mayores, lo que evidentemente debería conducir al reconocimiento de los ...factores de riesgo de desnutrición en los adultos mayores institucionalizados, que permita evaluar el riesgo de malnutrición y así facilitar la intervención nutricional precoz. Objetivo: evaluar el estado nutricional de los ancianos institucionalizados en el hogar de ancianos Carlos Castellanos Blanco de Pinar del Río, durante el 2016. Métodos: se realizó una investigación descriptiva y transversal. El universo estuvo constituido por los 282 ancianos de la institución social pinareña que se encontraban institucionalizados durante ese período. La muestra quedó integrada por 201 ancianos que cumplieron con los criterios de inclusión y exclusión establecidos en la investigación. Resultados: según índice de masa corporal y sexo la mayoría (61 %) se encuentran entre 21 - 23 Kg / m2de superficie corporal, la circunferencia del brazo según sexo demuestra que el 54,7 % de ancianos se encuentran entre límites normales (21- 22), de ellos el 34,8 % son mujeres, la medida de la circunferencia de la pantorrilla mostró que en el 77,1 % es < 31, con un mayor número de ancianos del sexo masculino (43,2%). Existió un discreto predominio de los ancianos bien nutridos con el 38,2 % sobre los que tienen riesgo de desnutrición (36,3 %), sin diferencias significativas con el sexo. Conclusiones: los ancianos institucionalizados en el hogar de ancianos, en el 2016 fueron evaluados como bien nutridos.
Aim
To explore the impact of the COVID‐19 pandemic on the mental health of nurses working in primary, secondary, and tertiary healthcare centers in Navarre (Spain).
Background
Healthcare workers, ...especially nurses, are at high risk for developing mental health problems during the COVID‐19 pandemic.
Introduction
Spain ranks among the European countries with the highest incidence of and mortality from COVID‐19 and has a 31% deficit in the number of nurses compared with the average for the European Union.
Methods
This was a cross‐sectional study involving 800 Registered Nurses in Navarre, Spain. Four standardized instruments, along with a self‐administered online questionnaire, were used to measure the impact in terms of depression, anxiety, insomnia, and posttraumatic stress disorder. The STROBE checklist for cross‐sectional studies was used to report this study.
Results
Of the 800 nurses, 68% had some level of depression, anxiety, insomnia, and distress, and of these, 38% had moderate or severe symptoms. Those who worked in hospital COVID units and in nursing homes showed a higher impact on their mental health.
Discussion
The sustained pressure that nurses have experienced in their work during the COVID‐19 pandemic has negatively affected their mental health.
Conclusion
This study found that nurses who worked in hospital COVID units and in nursing homes during the pandemic had worse mental health outcomes.
Implications for nursing/policy
Recommendations for nursing policy include the need to implement coaching and emotional programs to support nurses on the frontlines of the pandemic. There is also an urgent need for the implementation of national training programs to strengthen health emergency preparedness, improve response capacity, and increase the resilience of nurses to disasters.
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