Croton antisyphiliticus Mart. is a plant popularly used in folk medicine by traditional communities from Brazilian savannah to treat general inflammation. According to ethnopharmacological data, this ...specie can be considered a source of biologically active molecules for the development of new drugs. Thereby, this study reports the results of the dereplication approach of C. antisyphiliticus roots extracts and the in vivo evaluation of its potential antinociceptive and anti-inflammatory in albino Swiss mice. Based on HPLC coupled to Q-Exactive Orbitrap Mass Spectrometer and using GNPS, a total of thirteen polyphenolic compounds were noticed, including four compounds that have been reported for the first time in the genus Croton. Ethanolic and aqueous roots extracts demonstrated a dose-dependent inhibition for the number of writes, reduced pain induced by formalin and hyperalgesia induced by carrageenan. These extracts also reduced paw edema, cell migration, and myeloperoxidase activity, with effects similar to indomethacin and dexamethasone drugs.
Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on ...natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of
Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of
was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of
extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy.
As plataformas digitais de comunicação tornaram-se imprescindíveis como ferramentas para estreitar a comunicação entre a universidade e a comunidade externa. Este artigo relata o uso do YouTube® na ...ação de extensão WebCiência IQ-UFG realizada pela Universidade Federal de Goiás (UFG), analisando métricas de alcance social nas comunidades interna e externa à instituição. Os webinários promovidos tiveram um perfil amplo, com destaque para aqueles de cunho geral, associados à temática Ensino & Sociedade, e aos de fronteira da Química, em especial na área da saúde. As visualizações e likes não apresentaram diferenças significativas entre as modalidades de ensino remoto e presencial, com uma tendência de substituição de participantes internos à UFG por um público externo no ciclo de webinários após o retorno às atividades presenciais. A ação comprovou seu caráter extensionista, pois em todos os webinários houve a participação do público externo (todos os estados brasileiros e outros países).
O Programa Nacional de Alimentação Escolar (PNAE) é uma política pública que busca garantir a nutrição dosestudantes das escolas públicas e fortalecer a agricultura familiar. No entanto, o PNAE ...estabelece que, no mínimo, 30% dos recursos destinados à compra dos produtos que compõem a alimentação escolar devem ser provenientes da agricultura familiar local, o que muitas vezes não é atendido devido a desafios sanitários e à baixa qualidade dos produtos fornecidos.Foi desenvolvido um programa de extensão aplicando um método para formar as agricultoras familiares quanto às exigências sanitárias e à gestão de qualidade dos alimentos, possibilitando a preparação de oficinas e cartilhas pertinentes à gestão da qualidade e da produção, além da adaptação de técnicas de análise química e microbiológica para uso pela comunidade.Obteve sucesso na participação em programas de compras governamentais e na criação de um método para o desenvolvimento da gestão de qualidade na produção de alimentos.
Rhizobacteria are valuable sources of compounds that can be used for the integrated management of diseases in rice. Here, we aimed to explore the metabolism and organize and annotate the metabolites ...of
Bacillus
sp. and
Serratia marcescens
isolates using molecular networking and evaluate their fungicidal effects against
Magnaporthe oryzae
and
Bipolaris oryzae.
We obtained bacterial extracts after 6 and 16-h incubation via liquid–liquid extraction using ethyl acetate as solvent. We performed UHPLC-MS analysis and data processing using molecular networking and conducted biological assays in rice plants. Using the Global Natural Product Social spectral libraries, we annotated the following compounds: austinoneol, Phe-Pro,
N
-acetyl-
l
-leucine, Leu-Gly, Ile-Leu, Phe-Pro, 2,5-piperazinedione, 3-(1H-indol-3-methyl)-6-methyl-cyclo(
d
-Trp-
l
-Pro), and cholic acid. Results of the biological assays showed that the bacterial extracts reduced the mycelial growth of both pathogens in all treatments compared to the control. In the greenhouse setup, 8 days after the challenge for leaf gray spot and leaf blast, all treatments affected up to 4.4% of the leaf area, with an area under disease progress curve of 13.24, showing significant difference compared to the control, which affected 23% of the leaf area, with an AUDPC of 44.65. Our study provides potential new sources of natural products to be applied in the integrated management of rice.
Accidents involving snakes from Bothrops spp. and Crotalus spp. constitute the most important cause of envenomation in Brazil and Argentina. Musa spp. (banana) have been reported to be used in ...popular medicine against snakebite by the members of the Canudos Settlement, located in Goiás. In this way, the aim of this work was to evaluate the antivenom effect of the Ouro (AA), Prata (AAB), Prata-anã (AAB) and Figo (ABB) cultivars against in vitro (phospholipase, coagulation and proteolytic) and in vivo (lethality and toxicity) activities caused by the venoms and toxicity (Artemia salina nauplii and Danio rerio embryos) of Musa spp. as well as the annotation of chemical compounds possibly related to these activities. From the in vitro antiophidic tests with the sap, we observed 100% inhibition of the phospholipase and coagulant activities with the cultivars Prata-anã and Figo against the venoms of B. alternatus and C. d. collineatus, B. diporus and B. pauloensis, respectively, and neutralisation of the lethality against the B. diporus venom. It was observed that the cultivars of Musa spp. did not show toxicity against Artemia salina nauplii and Danio rerio embryos. The sap analysis via HPLC-MS/MS allowed the annotation of the 13 compounds: abscisic acid, shikimic acid, citric acid, quinic acid, afzelechin, Glp-hexose, glucose, sucrose, isorhamnetin-3-O-galactoside-6-raminoside, kaempferol-3-glucoside-3-raminoside, myricetin-3-O-rutinoside, procyanidin B1 and rutin. Therefore, it can be seen that Musa spp. is a potential therapeutic agent that can act to neutralise the effects caused by snakebites.
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•Coagulant, phospholipase, proteolytic and lethal activities were reduced by Musa spp. cultivars.•Cultivars saps were studied by UHPLC-Q-Orbitrap MS/MS and molecular networking analysis.•The saps are rich in organooxygen compounds, prenol lipid, carboxylic acid and flavonoids.•Musa spp. cultivars do not show toxicity against Artemia salina nauplii and Danio rerio embryos.•Musa spp. cultivars are a strategy against envenomation by Bothrops and Crotalus genus.
ABSTRACT Rice blast caused by the Magnaporthe oryzae pathogen is a major disease in this crop, and may cause devastating losses. This study aimed to investigate the profile of Waitea circinata ...mycorrhiza volatile compounds in antagonism to M. oryzae, under different growth periods and temperatures, using a completely randomized design. Volatile organic compounds were extracted by headspace solid phase microextraction and analyzed by gas chromatography mass spectrometry, while multidimensional scaling was used to compare the produced volatile organic compounds. The main compounds responsible by the antagonism to M. oryzae were longifolene, trans-β-farnesene, (Z)-α-bisabolene and δ-amorphene, which can be used as biofungicides and incorporated into rice blast management strategies.
RESUMO A brusone causada pelo patógeno Magnaporthe oryzae é a principal doença do arroz, e pode causar perdas devastadoras. Objetivou-se investigar o perfil de compostos voláteis da micorriza Waitea circinata no antagonismo a M. oryzae, em diferentes períodos e temperaturas, utilizando-se delineamento inteiramente casualizado. Compostos orgânicos voláteis foram extraídos por micro extração em fase sólida, no modo headspace, e analisados por cromatografia gasosa acoplada a espectrometria de massas, enquanto o escalonamento multidimensional foi usado para comparar os compostos orgânicos voláteis produzidos. Os principais compostos responsáveis pelo antagonismo a M. oryzae foram longifoleno, trans-β-farneseno, (Z)-α-bisaboleno e δ-amorfeno, que podem ser usados como biofungicidas e incorporados às estratégias de manejo da brusone do arroz.
In this paper, the chemical study of Hortia superba and antimycobacterial potential of Hortia species were investigated. Crude extracts and limonoids, alkaloids, dihydrocinnamic acid derivatives and ...coumarins isolated from Hortia superba, Hortia oreadica and Hortia brasiliana were evaluated against Mycobacterium tuberculosis H37Rv, Mycobacterium kansasii and Mycobacterium avium. The results obtained demonstrated an inhibitory effect of the dichloromethane extract of leaves of H. oreadica (MIC 31.25 µg mL-1), indolequinazoline (15.62 µg mL-1) and furoquinoline (31.25 µg mL-1) alkaloids, and dihydrocinnamic acid derivatives (62.50 µg mL-1), on the growth of M. tuberculosis. These results are promising in relation to the search for biologically active natural products and could be useful in the development of effective new drugs against mycobacteria.
This paper explores the medicinal plants used by Coqueiros Community, Brazil, for the treatment of various ailments. We apply the structured interview and the participant observation, and the local ...knowledge was analyzed using quantitative measures applied to the general sampling to contribute to selection of promising species for biotechnological development. This community cited 105 species from 53 plant families. The most representative families were Fabaceae, Asteraceae, Anacardiaceae, Myrtaceae, Annonaceae, Apocynaceae, Lamiaceae and Rutaceae. Among the species mentioned in this survey, 66,7% of them occur in the Cerrado as native vegetation. The species considered the most important by interviewees are those with action forthe largest number of health problems. Thus, the statistical indexes used here, such as Relative Impotance (RI), Informant Consensus Factor (ICF) and Relative Frequency of Citation (RFC), contributed to identify these species. To conclude, from this research we have known the potential of the Brazilian vegetation for the development of biotechnology.
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