Both the pollination control system and genetic distance are major factors in the utilization of crop heterosis. The recessive genic male sterile line (RGMS) 7-7365A (Bnms3ms3ms4ms4) has been widely ...applied to hybrid seed production because it can generate a completely male sterile population by crossing with the 7-7365C temporary line (Bnms3ms3rfrf). In this study, the sterile genes of 7-7365A were transferred to the new Brassica napus lines 7-749 and 7-750 with a high content of subgenomes by backcross breeding. We used the amplified fragment length polymorphism (AFLP) technique combined with bulk segregant analysis (BSA) to identify markers linked to the BnMs4 gene. Twelve AFLP markers linked to the BnMs4 gene were identified. Of them, SA06MG09 and P08MG16 were the closest makers, which were on either side of the gene at a distance of 0.9 and 0.8 cM, respectively. Twenty AFLP primer combinations were used to screen the F2, BC1F3, and BC2F4 populations from the breeding program, and the markers linked to the BnMs3 and BnMs4 genes were used to screen the BC2F4 populations. As a result, we obtained two types of improved sterile lines, 7-749A and 7-750A, and their indexes of subgenomic components (ISG) were 44.2–49.8 and 20.2–26.6%, respectively. The combining ability analyses of seed yield character were conducted in the crosses from the three sterile lines and ten restorers within a random block design in three environments for two successive years. The general combining ability (GCA) of the two improved sterile lines were significantly higher than the GCA of 7-7365A in every environment tested. The two improved sterile lines had stability in seed yield, and they will be used in the future for hybrid seed production.
Abstract
Background
BRD
7 is a member of bromodomain‐containing protein and was found to be a cofactor of
P
53. Down‐regulation of
BRD
7 has been shown in nasopharyngeal carcinoma cell lines and ...tissues. However, the clinical role of
BRD
7 in colorectal cancer remains unknown.
Materials and methods
Real‐time
PCR
, Western blotting analysis and immunohistochemistry were employed to examine
BRD
7 expression in
CRC
cell lines/tissues compared with normal epithelia cells/adjacent non‐tumorous tissues. In addition, statistical analyses were applied to evaluate the diagnostic value and associations of
BRD
7 expression with clinical parameters of patient samples.
Results
BRD
7 was down‐regulated in colorectal cancer cell lines and cancerous tissues compared with that in normal colon epithelial cells and adjacent noncancerous tissue samples.
BRD
7 protein expression was positively correlated with clinical stage (
P
< 0·001), T classification (
P
= 0·001),
N
classification (
P
< 0·001), M classification (
P
< 0·001) and pathologic differentiation (
P
= 0·008). Patients with low/none
BRD
7 expression had shorter overall survival time than those with higher
BRD
7 expression. Univariate and multivariate analyses indicated
BRD
7 expression was an independent prognostic factor (
P
<
0·001).
Conclusion
BRD
7 may serve as a potential prognostic biomarker of human colorectal cancer.
Nor did it replicate in a GWAS from a similar high-risk population (National Cancer Institute) or in a GWAS from a low-risk population (Beijing). ...the original finding was likely the result of ...inadequate control for population stratification using the genetically unmatched subjects or, less likely, could have been due to chance alone.
Monodisperse Ce sub(1-x)Zr sub(x)O sub(2) nanocrystals have been synthesized using a simple two-phase approach; adjusting the ratio of precursors used, amount of capping agent used, reaction time and ...temperature affords precise control over their composition, structure and size. Size-dependent enhancement of oxygen-storage capacity and kinetics of oxygen storage and release were observed. Systematic studies were conducted in order to understand the size-dependent enhancement of these properties. This work provides important insights into the synthesis and fundamental understanding of multi-component nanocrystals with a large variety of applications.
Monodisperse Ce^sub 1-x^Zr^sub x^O2 nanocrystals have been synthesized using a simple two-phase approach; adjusting the ratio of precursors used, amount of capping agent used, reaction time and ...temperature affords precise control over their composition, structure and size. Size-dependent enhancement of oxygen-storage capacity and kinetics of oxygen storage and release were observed. Systematic studies were conducted in order to understand the size-dependent enhancement of these properties. This work provides important insights into the synthesis and fundamental understanding of multi-component nanocrystals with a large variety of applications.PUBLICATION ABSTRACT
N
-methyladenosine (m
A) is an abundant modification in eukaryotic mRNA, regulating mRNA dynamics by influencing mRNA stability, splicing, export, and translation. However, the precise m
A regulating ...machinery still remains incompletely understood. Here we demonstrate that ZC3H13, a zinc-finger protein, plays an important role in modulating RNA m
A methylation in the nucleus. We show that knockdown of Zc3h13 in mouse embryonic stem cell significantly decreases global m
A level on mRNA. Upon Zc3h13 knockdown, a great majority of WTAP, Virilizer, and Hakai translocate to the cytoplasm, suggesting that Zc3h13 is required for nuclear localization of the Zc3h13-WTAP-Virilizer-Hakai complex, which is important for RNA m
A methylation. Finally, Zc3h13 depletion, as does WTAP, Virilizer, or Hakai, impairs self-renewal and triggers mESC differentiation. Taken together, our findings demonstrate that Zc3h13 plays a critical role in anchoring WTAP, Virilizer, and Hakai in the nucleus to facilitate m
A methylation and to regulate mESC self-renewal.
Bacillus licheniformis CICIM B5102 was used for the commercial production of alkaline protease. The full-length gene apr encoding the alkaline protease was amplified via polymerase chain reaction ...(PCR) using the genomic DNA of B. licheniformis CICIM B5102 as a template. The apr gene was cloned into plasmid pUB110, resulting in the recombinant plasmid pUB-apr, which was then transformed into Bacillus amyloliquefaciens CICIM B4803. The protease productivity was significantly improved in the transformants of B. amyloliquefaciens CICIM B4803. A transformant with high alkaline protease productivity was selected, and the alkaline protease productivity of the strain increased by 46% when compared with that of wild-type B. licheniformis CICIM B5102.
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