Alcohol‐exposed children display delayed motor development and impaired fine‐ and gross‐motor skills, including deficits in the maintenance of balance. In a recent study, we assessed the contribution ...of visual, somatosensory, and vestibular information to the ability to maintain balance. Our findings suggested that alcohol‐exposed children were overly reliant on somatosensory information and were unable to compensate by using the visual and/or vestibular systems. To understand the nature of these observed balance deficits, corrective postural reactions were examined by exposing standing subjects to rapid toe‐up movements of the support surface. Subjects for this study were alcohol‐exposed (ALC) and normal control (NC) children matched for age and sex. Postural reactions were quantified by measuring electromyographic activity of the triceps surae and anterior tibialis muscles. Analyses revealed no differences between the ALC and NC groups on short‐ and medium‐latency electromyographic responses, which are thought to be involuntary mono‐ and polysynaptic spinal reflexes, respectively. However, when compared with the NC group, the ALC group displayed increased long‐latency responses, which are thought to involve a transcortical pathway. Although we are not able to rule out the possibility of additional peripheral (e.g., vestibular) disturbance as a contributing factor to postural instability, our findings suggest that the balance deficits seen in alcohol‐exposed children are, at least in part, central in nature.
Standard activated clotting time (ACT) tests have a poor correlation to bivalirudin levels, leading to uncertainty regarding adequate anticoagulation in percutaneous coronary intervention patients. ...We tested a Thrombelastograph (TEG) ecarin clotting time (ECT) assay for sensitivity to bivalirudin using blood from 80 patients undergoing interventional cardiology procedures with bivalirudin anticoagulation. This was compared to a standard Hemochron ACT assay using diatomaceous earth. With the TEG assay, the direct thrombin activator, ecarin, was used to initiate coagulation and measured as the reaction time. Plasma samples were evaluated for bivalirudin by a chromogenic assay at an independent hematological laboratory. Linear regression of the standard ACT versus bivalirudin level gave an r = 0.306 whereas the TEG ECT gave a much higher r2 = 0.746 (both P < 0.0001). The TEG ECT should prove more useful than the standard ACT for monitoring bivalirudin anticoagulation across the clinically therapeutic range.
Ten subjects completed a series of goal-directed arm flexion movements unexpectedly perturbed by three different types of mechanical load. Examination of electromyograph (EMG) waveforms and kinematic ...information collected during randomly distributed test trials facilitated investigation into the interaction between loading conditions and the response-associated EMG innervation patterns. Results of the EMG waveform analysis revealed that inertial and spring loads produced cocontraction and triphasic activation patterns, respectively. Unexpected application of a stretched-spring load, which produced a change in initial torque values without changing the rate of loading, also resulted in the use of a triphasic activation pattern. These different EMG patterns were observed while movement displacement for all three loads fell within the limits of the target area.
In this and a previous experiment it has been observed that subjects produce innervation patterns (EMG) that are load specific, i.e., they produce concentration patterns for movements made with ...inertial loads and triphasic patterns for movements made with elastic loads. The protocol of these experiments prevented any adaptive responses to the load changes, therefore, it was assumed that pattern matching to load type was a real time updating response of the peripheral feedback systems. This updating response was assumed to be a mechanism for fine tuning the muscle torque by regulation of the mechanical impedance (stiffness) of the limb. Using a standard equation of motion, it was shown that the velocity is equal to the ratio of the muscle torque to the mechanical impedance. Substitution of this ratio for the ordinate of the scaled phase diagrams was then suggested as a real time updating mechanism to account for the scaled phase invariance recorded in this experiment and in the experiment reported by Ruitenbeek, J.C., Biol. Cybernetics 51 (1984) 11-20.
Two experiments investigated whether unexpected and differential loading of a rapid, unsighted arm movement resulted in the central nervous system (CNS) regulating limb stiffness by modifying the ...associated neuromuscular activity. In Experiment 1, subjects completed multiple, spring-loaded training trials until a prespecified criterion of learning was attained. On selected trials, the spring load was unexpectedly replaced by an inertial load. Results indicated that to maintain positional accuracy during this inertial load trial, limb stiffness was increased by coactivating the antagonist muscles, i.e. by changing the associated neuromuscular activity from a predominantly triphasic pattern to one of coactivation. In Experiment 2, the sequence of loading was reversed producing a change in the required limb stiffness from a relatively high to low level. This change was observed as a pattern of coactivation being replaced by a triphasic activity pattern. These results support the notion that limb stiffness is regulated primarily through modification of the neuromuscular activity pattern prior to movement termination. It was also demonstrated that the size of the unexpected load did not affect the basic activation pattern selected by the CNS. It is proposed that the signal which triggers the CNS to regulate limb stiffness is based on peripheral information generated as a result of agonist activity occurring during the first part of the movement.
Diabetic subjects completed a series of rapid, goal-directed arm movements under two conditions of unexpected external loading. Evaluation of electromyographic (EMG) patterns revealed that ...cocontraction and triphasic activity were predominantly associated with inertial and spring loading, respectively. During inertial load responses some EMG patterns indicated a modified cocontraction pattern. Response accuracy was unaffected by type of load but movement time was greater for the inertial load condition.
The influence of amount and type of exploratory activity upon 3 male and 5 female undergraduates' ability to detect the symmaterical or asymmetrical characteristics of individually presented plastic ...nonrepresentational shapes was examined. A decrease in scanning time and number of identification errors between the first and fourth days of testing indicates that extended perceptual experience plays a major role in the efficient pick-up and utilization of haptic information. A signal-detection analysis demonstrated that the use of a simultaneous-apprehension scanning strategy resulted in greater sensitivity to asymmetrical shapes than did the use of a simultaneous-apprehension scan. The data support the hypothesis that experience, the form parameter of symmetry, and the type of scanning strategy used to explore a stimulus function together to influence haptic perception of nonrepresentational shapes.
In Cambodia, dengue virus (DENV) was first isolated in 1963 and has become endemic with peak epidemic during raining season. Since 2000, the Dengue National Control Program has reported from 10,000 ...to 40,000 cases per year with fatality rates ranging from 0.7 to 1.7. All four dengue serotypes are found circulating in Cambodia with alternative predominance of serotypes DENV-2 and DENV-3. The DENV-1 represents from 5% to 20% of all circulating viruses, depending upon the year. In this work, 79 clinical strains of DENV-1 were isolated between 2000 and 2009 and their genome fully sequenced. Four distinct lineages with different dynamics were identified. The main evolutionary drive was negative selective pressure but each lineage was characterized by the presence of specific mutations acquired through evolution. Coexistence, extinction and replacement of lineages occurred over the 10-year period. Lineages 1, 2 and 3 were all detected since 2000–2002 and disappeared in 2003, 2004–2005 and 2007, respectively. Lineages 1 and 2 displayed different dynamics. Lineage 1 was very diverse whereas lineage 2 was very homogeneous. Lineage 4 which derived from lineage 3 in 2003 remained the only one at the end of the sampling period in 2008–2009 owing to a selective sweep. The lineages dynamic of DENV-1 viruses and consequences for molecular epidemiology are discussed.
Time series analysis was used to investigate the hypothesis that during acquisition of a motor skill, knowledge of results (KR) information is used to generate a stable internal referent about which ...response errors are randomly distributed. Sixteen subjects completed 50 acquisition trials of each of three movements whose spatial-temporal characteristics differed. Acquisition trials were either blocked, with each movement being presented in series, or randomized, with the presentation of movements occurring in random order. Analysis of movement time data indicated the contextual interference effect reported in previous studies was replicated in the present experiment. Time series analysis of the acquisition trial data revealed the majority of individual subject response patterns during blocked trials were best described by a model with a temporarily stationary, internal reference of the criterion and systematic, trial-to-trial variation of response errors. During random trial conditions, response patterns were usually best described by a "White-noise" model. This model predicts a permanently stationary, internal reference associated with randomly distributed response errors that are unaffected by KR information. These results are not consistent with previous work using time series analysis to describe motor behavior (Spray & Newell, 1986).
