The COVID-19 pandemic presented instructors and learners with novel challenges related to the delivery and consumption of instructional content. Within academic libraries, these changes have resulted ...in an expanded reliance on asynchronous learning content delivered through a variety of instructional approaches. The study described herein was designed to evaluate the effectiveness of a blended and flipped program of information literacy (IL) instruction. Deploying a pre/post-test methodology within a quasi-experimental study of student learning outcomes, this research compares achievement across two cohorts of students receiving IL instruction as part of a first-year writing program (FYWP) requirement. While one cohort participated in a multi-shot, in-person, synchronous instructional program during the pre-pandemic period, another cohort participated in a blended and flipped instructional program delivered under pandemic-related restrictions. Comparative analysis revealed net positive achievement outcomes for both the pre-pandemic and pandemic cohorts, as well as interesting points of contrast between the two groups. In particular, students in the pandemic cohort arrived to their first year with less research experience than their pre-pandemic peers—signaling a gap in knowledge that pandemic-era library instruction must overcome. Fortunately, the blended and flipped instructional program experienced by the pandemic cohort proved successful in helping those students overcome their research experience gap, while delivering additional benefits that help confirm previous research in this area of practice.
A 10-O-deacetylbaccatin III 10-O-acetyltransferase biocatalyst from Taxus plants was expressed in bacteria whole-cells that were fed 10-O-deacetylbaccatin III and cyclopropane carboxylic acid. ...Product analysis by qualitative LC/ESI-MS suggested that the C10-acylated products baccatin III, 10-O-n-propionyl-10-O-deacetylbaccatin III, and 10-O-cyclopropanecarbonyl-10-O-deacetylbaccatin III were made in vivo. The results implied that the cells provided non-natural cyclopropanecarbonyl CoA, from a broad-specificity CoA ligase, and natural products, acetyl CoA and n-propionyl CoA, from reserves in the bacteria for use by acyltransferase to acylate 10-O-deacetylbaccatin III in vivo. The 10-acyl-10-O-deacetylbaccatin III are precursors used to synthesize new-generation paclitaxel analogs SB-T-1214 and SB-T-121303, which are effective against cancer cells resistant to paclitaxel and its drug derivatives. The kcat and KM of the acyltransferase for cyclopropanecarbonyl CoA (0.83 s−1, 0.15 M) and n-propionyl CoA (1.2 s−1, 0.15 M) guided scale-up efforts. The 10-acyl-10-O-deacetylbaccatin III analogs (∼45 mg each) were made in vitro by the acyltransferase when incubated with the commercial taxane 10-O-deacetylbaccatin III and synthesized cyclopropanecarbonyl or n-propionyl CoA. The structures of the 10-acyl products were verified by NMR analyses that confirmed C10 acylation of the taxane substrate. LC/ESI-MS/MS analysis also supported the identities of the biocatalyzed products. This effort provides a biocatalysis framework to produce new-generation taxane precursors.
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•Whole-cell biocatalysis to make a non-natural taxane analog via a non-natural acyl CoA.•In vitro biocatalytic production of new-generation paclitaxel precursors at mg-scale.•Demonstrated the flexibility of a short-chain n-alkanoyltransferase for cyclopropanecarbonyl.
Alkylpyrazines are important heterocyclic compounds used as flavorants in food and beverage industries. In this study, a regioselective semibiocatalytic process was developed to synthesize ...2-ethyl-3,5-dimethylpyrazine (235-EDMP) over its 3-ethyl-2,5-dimethyl pyrazine (325-EDMP) isomer and vice versa. We initially explored how sterics could direct the coupling orientations between diamines and diketones to access 235- or 325-EDMP selectively. Also, the physical parameters of the reaction conditions were changed, such as reduced temperature, the order-of-addition of the reactants, and supplementation with chiral zeolites to template the orientation of the coupling partners to direct the reaction regiochemistry. Each reaction trial resulted in 50:50 mixtures of the EDMP isomers. An alternative approach was explored to control the regioselectivity of the reactions; α-hydroxy ketones replaced the diketones as the electrophilic coupling reactant used in previous trial experiments. The hydroxy ketone reactants were made biocatalytically with pyruvate decarboxylase. The coupling reaction between 2-hydroxypentan-3-one and propane-1,2-diamine resulted in the desired 235-EDMP at >70% (∼77 mg) relative to 325-EDMP in the mixture. The 3-hydroxypentan-2-one congener was biocatalyzed and reacted with propane-1,2-diamine as a proof of principle to synthesize 325-EDMP (∼60% relative abundance, ∼73 mg) over 235-EDMP. These results suggested a mechanism that was directed by the hydroxy ketone electrophilicity and the sterics at the diamine nucleophilic centers.
Low-use tangible print collections represent a long-standing problem for academic libraries. Expanding on the previous research aimed at leveraging machine learning (ML) toward predicting patterns of ...collection use, this study explores the potential for adaptive boosting (AdaBoost) as a foundation for developing actionable predictive models of print title use. This study deploys the AdaBoost algorithm, with random forests used as the base classifier, via the adabag package for R. Methodological considerations associated with dataset congruence, as well as sample-based modeling versus novel data modeling, are explored in relation to four AdaBoost models that are trained and tested. Results of this study show AdaBoost as a promising ML solution for predictive modeling of print collections, with the central model of interest able to accurately predict use in over 85% of cases. This research also explores peripheral questions of interest related to general considerations when evaluating ML models, as well as the compatibility of similar models trained with e-book versus print book usage data.
Peripheral nerve blocks can be performed using ultrasound guidance. It is not yet clear whether this method of nerve location has benefits over other existing methods. This review was originally ...published in 2009 and was updated in 2014.
The objective of this review was to assess whether the use of ultrasound to guide peripheral nerve blockade has any advantages over other methods of peripheral nerve location. Specifically, we have asked whether the use of ultrasound guidance:1. improves success rates and effectiveness of regional anaesthetic blocks, by increasing the number of blocks that are assessed as adequate2. reduces the complications, such as cardiorespiratory arrest, pneumothorax or vascular puncture, associated with the performance of regional anaesthetic blocks
In the 2014 update we searched the Cochrane Central Register of Controlled Trials (CENTRAL; 2014, Issue 8); MEDLINE (July 2008 to August 2014); EMBASE (July 2008 to August 2014); ISI Web of Science (2008 to April 2013); CINAHL (July 2014); and LILACS (July 2008 to August 2014). We completed forward and backward citation and clinical trials register searches.The original search was to July 2008. We reran the search in May 2015. We have added 11 potential new studies of interest to the list of 'Studies awaiting classification' and will incorporate them into the formal review findings during future review updates.
We included randomized controlled trials (RCTs) comparing ultrasound-guided peripheral nerve block of the upper and lower limbs, alone or combined, with at least one other method of nerve location. In the 2014 update, we excluded studies that had given general anaesthetic, spinal, epidural or other nerve blocks to all participants, as well as those measuring the minimum effective dose of anaesthetic drug. This resulted in the exclusion of five studies from the original review.
Two authors independently assessed trial quality and extracted data. We used standard Cochrane methodological procedures, including an assessment of risk of bias and degree of practitioner experience for all studies.
We included 32 RCTs with 2844 adult participants. Twenty-six assessed upper-limb and six assessed lower-limb blocks. Seventeen compared ultrasound with peripheral nerve stimulation (PNS), and nine compared ultrasound combined with nerve stimulation (US + NS) against PNS alone. Two studies compared ultrasound with anatomical landmark technique, one with a transarterial approach, and three were three-arm designs that included US, US + PNS and PNS.There were variations in the quality of evidence, with a lack of detail in many of the studies to judge whether randomization, allocation concealment and blinding of outcome assessors was sufficient. It was not possible to blind practitioners and there was therefore a high risk of performance bias across all studies, leading us to downgrade the evidence for study limitations using GRADE. There was insufficient detail on the experience and expertise of practitioners and whether experience was equivalent between intervention and control.We performed meta-analysis for our main outcomes. We found that ultrasound guidance produces superior peripheral nerve block success rates, with more blocks being assessed as sufficient for surgery following sensory or motor testing (Mantel-Haenszel (M-H) odds ratio (OR), fixed-effect 2.94 (95% confidence interval (CI) 2.14 to 4.04); 1346 participants), and fewer blocks requiring supplementation or conversion to general anaesthetic (M-H OR, fixed-effect 0.28 (95% CI 0.20 to 0.39); 1807 participants) compared with the use of PNS, anatomical landmark techniques or a transarterial approach. We were not concerned by risks of indirectness, imprecision or inconsistency for these outcomes and used GRADE to assess these outcomes as being of moderate quality. Results were similarly advantageous for studies comparing US + PNS with NS alone for the above outcomes (M-H OR, fixed-effect 3.33 (95% CI 2.13 to 5.20); 719 participants, and M-H OR, fixed-effect 0.34 (95% CI 0.21 to 0.56); 712 participants respectively). There were lower incidences of paraesthesia in both the ultrasound comparison groups (M-H OR, fixed-effect 0.42 (95% CI 0.23 to 0.76); 471 participants, and M-H OR, fixed-effect 0.97 (95% CI 0.30 to 3.12); 178 participants respectively) and lower incidences of vascular puncture in both groups (M-H OR, fixed-effect 0.19 (95% CI 0.07 to 0.57); 387 participants, and M-H OR, fixed-effect 0.22 (95% CI 0.05 to 0.90); 143 participants). There were fewer studies for these outcomes and we therefore downgraded both for imprecision and paraesthesia for potential publication bias. This gave an overall GRADE assessment of very low and low for these two outcomes respectively. Our analysis showed that it took less time to perform nerve blocks in the ultrasound group (mean difference (MD), IV, fixed-effect -1.06 (95% CI -1.41 to -0.72); 690 participants) but more time to perform the block when ultrasound was combined with a PNS technique (MD, IV, fixed-effect 0.76 (95% CI 0.55 to 0.98); 587 participants). With high levels of unexplained statistical heterogeneity, we graded this outcome as very low quality. We did not combine data for other outcomes as study results had been reported using differing scales or with a combination of mean and median data, but our interpretation of individual study data favoured ultrasound for a reduction in other minor complications and reduction in onset time of block and number of attempts to perform block.
There is evidence that peripheral nerve blocks performed by ultrasound guidance alone, or in combination with PNS, are superior in terms of improved sensory and motor block, reduced need for supplementation and fewer minor complications reported. Using ultrasound alone shortens performance time when compared with nerve stimulation, but when used in combination with PNS it increases performance time.We were unable to determine whether these findings reflect the use of ultrasound in experienced hands and it was beyond the scope of this review to consider the learning curve associated with peripheral nerve blocks by ultrasound technique compared with other methods.
Demand-driven acquisition (DDA) programs are playing an increasingly important role in academic libraries. However, the literature surrounding this topic illustrates the wide-ranging, and frequently ...unpredictable, results of DDA implementation. As uncertainty abounds, librarians continue to seek out deeper understandings of those processes driving the use and purchase of DDA materials. Implicit in this search is a desire to understand how local environmental factors and user preferences dictate broader collection use and purchasing patterns. A small number of these studies have sought deeper insights through predictive modeling, though success has been limited. Following this line of inquiry, this study explores how machine learning might enable more effective collection development and management strategies through the predictive modeling of complex collection use and purchasing patterns. This research describes a replicable implementation of an adaptive boosting (AdaBoost) model that predicts the likelihood of DDA titles being triggered for purchase. The predictive capacity of this model is compared against a more traditional logistic regression model. This study's results show that the AdaBoost model possesses much higher predictive capacity than a regression-based model informed by the same set of predictors. The AdaBoost algorithm, once trained with local DDA data, provides accurate predictions in 82% of cases.
Although research on human-mediated exchanges of species has substantially intensified during the last centuries, we know surprisingly little about temporal dynamics of alien species accumulations ...across regions and taxa. Using a novel database of 45,813 first records of 16,926 established alien species, we show that the annual rate of first records worldwide has increased during the last 200 years, with 37% of all first records reported most recently (1970-2014). Inter-continental and inter-taxonomic variation can be largely attributed to the diaspora of European settlers in the nineteenth century and to the acceleration in trade in the twentieth century. For all taxonomic groups, the increase in numbers of alien species does not show any sign of saturation and most taxa even show increases in the rate of first records over time. This highlights that past efforts to mitigate invasions have not been effective enough to keep up with increasing globalization.
Site-directed mutations and substrate analogues were used to gain insights into the branch-point reaction of the 3,5-dihydro-5-methylidene-4 H-imidazol-4-one (MIO)-tyrosine aminomutase from Oryza ...sativa ( OsTAM). Exchanging the active residues of OsTAM (Y125C/N446K) for those in a phenylalanine aminomutase TcPAM altered its substrate specificity from tyrosine to phenylalanine. The aminomutase mechanism of OsTAM surprisingly changed almost exclusively to that of an ammonia lyase making cinnamic acid (>95%) over β-phenylalanine Walter, T., et al. (2016) Biochemistry 55, 3497-3503. We hypothesized that the missing electronics or sterics on the aryl ring of the phenylalanine substrate, compared with the sizable electron-donating hydroxyl of the natural tyrosine substrate, influenced the unexpected lyase reactivity of the OsTAM mutant. The double mutant was incubated with 16 α-phenylalanine substituent analogues of varying electronic strengths and sterics. The mutant converted each analogue principally to its acrylate with ∼50% conversion of the p-Br substrate, making only a small amount of the β-amino acid. The inner loop structure over the entrance to the active site was also mutated to assess how the lyase and mutase activities are affected. An OsTAM loop mutant, matching the loop residues of TcPAM, still chiefly made >95% of the acrylate from each substrate. A combined active site:loop mutant was most reactive but remained a lyase, making 10-fold more acrylates than other mutants did. While mutations within the active site changed the substrate specificity of OsTAM, continued exploration is needed to fully understand the interplay among the inner loop, the substrate, and the active site in defining the mutase and lyase activities.
We study symmetry-enriched topological (SET) phases in 2+1 space-time dimensions with spatial reflection and/or time-reversal symmetries. We provide a systematic construction of a wide class of ...reflection and time-reversal SET phases in terms of a topological path integral defined on general space-time manifolds. An important distinguishing feature of different topological phases with reflection and/or time-reversal symmetry is the value of the path integral on non-orientable space-time manifolds. We derive a simple general formula for the path integral on the manifold
Σ
2
×
S
1
, where
Σ
2
is a two-dimensional non-orientable surface and
S
1
is a circle. This also gives an expression for the ground state degeneracy of the SET on the surface
Σ
2
that depends on the reflection symmetry fractionalization class, generalizing the Verlinde formula for ground state degeneracy on orientable surfaces. Consistency of the action of the mapping class group on non-orientable manifolds leads us to a constraint that can detect when a time-reversal or reflection SET phase is anomalous in (2+1)D and, thus, can only exist at the surface of a (3+1)D symmetry protected topological (SPT) state. Given a (2+1)D reflection and/or time-reversal SET phase, we further derive a general formula that determines which (3+1)D reflection and/or time-reversal SPT phase hosts the (2+1)D SET phase as its surface termination. A number of explicit examples are studied in detail.
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