Developing an anti-infective shape-memory hemostatic sponge able to guide in situ tissue regeneration for noncompressible hemorrhages in civilian and battlefield settings remains a challenge. Here we ...engineer hemostatic chitosan sponges with highly interconnective microchannels by combining 3D printed microfiber leaching, freeze-drying, and superficial active modification. We demonstrate that the microchannelled alkylated chitosan sponge (MACS) exhibits the capacity for water and blood absorption, as well as rapid shape recovery. We show that compared to clinically used gauze, gelatin sponge, CELOX™, and CELOX™-gauze, the MACS provides higher pro-coagulant and hemostatic capacities in lethally normal and heparinized rat and pig liver perforation wound models. We demonstrate its anti-infective activity against S. aureus and E. coli and its promotion of liver parenchymal cell infiltration, vascularization, and tissue integration in a rat liver defect model. Overall, the MACS demonstrates promising clinical translational potential in treating lethal noncompressible hemorrhage and facilitating wound healing.
Due to their lower systemic toxicity, faster kidney clearance and higher tumor accumulation, ultrasmall gold nanoparticles (less than 10 nm in diameter) have been proved to be promising in biomedical ...applications. However, their potential applications in cancer imaging and treatment have not been reviewed yet. This review summarizes the efforts to develop systems based on ultrasmall gold nanoparticles for use in cancer diagnosis and therapy. First, we describe the methods for controlling the size and surface functionalization of ultrasmall gold nanoparticles. Second, we review the research on ultrasmall gold nanoparticles in cancer imaging and treatment. Specifically, we focus on the applications of ultrasmall gold nanoparticles in tumor visualization and bioimaging in different fields such as magnetic resonance imaging, photoacoustic imaging, fluorescence imaging, and X-ray scatter imaging. We also highlight the applications of ultrasmall gold nanoparticles in tumor chemotherapy, radiotherapy, photodynamic therapy and gene therapy.
The fruit of Forsythia suspensa (Thunb.) Vahl, named Forsythiae Fructus (Lian-Qiao), is a well-known traditional Chinese medicine (TCM) used for clearing away heat and toxic material, eliminating the ...mass and relieving swelling.
This study aims to observe the attenuation of the water extract of Forsythiae Fructus (FSE) on carbon tetrachloride (CCl4)-induced hepatic fibrosis in male C57BL/6 mice.
Hepatic fibrosis was induced in male C57BL/6 mice by intraperitoneal injection with 2 ml/kg CCl4 (mixed 1: 3 in olive oil) twice a week for 4 weeks. At the same time, the mice were orally given with FSE (1, 2 g/kg) every day for 4 weeks. Serum biochemical parameters, gene and protein expression related to liver fibrosis were analyzed. The contents of forsythiaside A and forsythin in FSE were measured by high-performance liquid chromatography (HPLC).
Results of serum alanine/aspartate aminotransferase (ALT/AST) activity and liver histological evaluation both showed the protection of FSE against CCl4-induced liver injury. Further, the anti-fibrotic effects of FSE was evidenced by the results of Masson's trichrome and Sirius red staining, liver hydroxyproline content, and serum amounts of hyaluronic acid, laminin, collagen Ⅳ and type III procollagen (PCIII). FSE also reduced the expression of α-smooth muscle actin (α-SMA) in livers from CCl4-injured mice. Additionally, FSE decreased the increased hepatic expression of fibroblast-specific protein 1 (FSP1) and vimentin induced by CCl4 in mice.
FSE attenuates CCl4-induced liver fibrosis in mice by inhibiting hepatic stellate cells (HSCs) activation, reducing hepatic extracellular matrix (ECM) disposition and reversing epithelial-mesenchymal transition (EMT).
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Cellular transition to hypoxia following tissue injury, has been shown to improve angiogenesis and regeneration in multiple tissues. To take advantage of this, many hypoxia-mimicking scaffolds have ...been prepared, yet the oxygen access state of implanted artificial small-diameter vascular grafts (SDVGs) has not been investigated. Therefore, the oxygen access state of electrospun PCL grafts implanted into rat abdominal arteries was assessed. The regions proximal to the lumen and abluminal surfaces of the graft walls were normoxic and only the interior of the graft walls was hypoxic. In light of this differential oxygen access state of the implanted grafts and the critical role of vascular regeneration on SDVG implantation success, we investigated whether modification of SDVGs with HIF-1α stabilizer dimethyloxalylglycine (DMOG) could achieve hypoxia-mimicking responses resulting in improving vascular regeneration throughout the entirety of the graft wall. Therefore, DMOG-loaded PCL grafts were fabricated by electrospinning, to support the sustained release of DMOG over two weeks. In vitro experiments indicated that DMOG-loaded PCL mats had significant biological advantages, including: promotion of human umbilical vein endothelial cells (HUVECs) proliferation, migration and production of pro-angiogenic factors; and the stimulation of M2 macrophage polarization, which in-turn promoted macrophage regulation of HUVECs migration and smooth muscle cells (SMCs) contractile phenotype. These beneficial effects were downstream of HIF-1α stabilization in HUVECs and macrophages in normoxic conditions. Our results indicated that DMOG-loaded PCL grafts improved endothelialization, contractile SMCs regeneration, vascularization and modulated the inflammatory reaction of grafts in abdominal artery replacement models, thus promoting vascular regeneration.
Abstract
To solve the problem of contour error (CE) arising from the dual‐axis coupling of the
X
–
Y
coordinate planar motor stage under reciprocating motion conditions in integrated circuit (IC) ...manufacturing, the following two perspectives are considered: indirect intervention from a single‐axis servo tracking error (TE) control and direct intervention from a dual‐axis cross‐coupling control (CCC). An improved motion controller is designed using the iterative learning control (ILC) principle in conjunction with the motion characteristics of the dual‐axis mechanism. Specifically, the variable gain iterative learning controller with a variable forgetting factor (VFF‐VGILC) is designed to address the characteristics of single‐axis trajectory repetition and variable velocity planning in dual‐axis coupling. The significance of the VFF‐VGILC is to increase the servo control bandwidth of a single axis. On the other hand, the cross‐coupled iterative learning controller (CCILC) is designed on the dual‐axis cross‐coupling, while a pre‐compensation strategy is introduced in the CCC structure to form the pre‐CCILC. After experimental designing and testing are performed, the
X
–
Y
coordinate planar motor stage can effectively reduce the CE under various trajectory movements compared with the traditional controller. The trajectory curves drawn can meet the motion control requirements better and effectively enhance the motion control capability of the mechanism.
Rapid endothelialization is a key factor that determines the success of small-diameter vascular grafts as an artery substitute in the treatment of cardiovascular diseases. Aimed to facilitate ...vascular regeneration, we developed a vascular scaffold loaded with resveratrol, which is a natural compound extracted from plants and showed multifaceted effects in cardiovascular protection. The tubular poly(ε-caprolactone) (PCL) scaffold was prepared by electrospinning with resveratrol in the PCL solution. In vitro assay demonstrated that resveratrol could be released from the scaffolds in a sustained and controlled manner. Cell culture results indicated that the migration of endothelial cells (ECs), nitric oxide production, and the ability of tube formation increased in the resveratrol-containing PCL scaffold groups compared with the PCL control. Meanwhile, the level of tumor necrosis factor (TNF)-α, the main proinflammatory factor secreted from macrophages, was reduced, and the messenger RNA expressions of the M2 macrophage-related genes were increased in the resveratrol-containing group. Further, in vivo implantation was performed by replacing rat abdominal aorta. We observed fast endothelialization and enhanced vascular regeneration in rats with resveratrol-containing scaffolds. The presence of resveratrol also induced a large number of M2 macrophages to infiltrate into the graft wall. Taken together, the incorporation of resveratrol into the PCL grafts enhanced the vascular regeneration by modulation of ECs and macrophages.
Since the invention of the train, the problem of train noise has been a constraint on the development of trains. With increases in train speed, the main noise from high-speed trains has changed from ...rolling noise to aerodynamic noise, and the noise level and noise frequency range have also changed significantly. This paper provides a comprehensive overview of recent advances in the development of high-speed train noise. Firstly, the train noise composition is summarized; next, the main research methods for train noise, which include real high-speed train noise tests, wind tunnel tests, and numerical simulations, are reviewed and discussed. We also discuss the current methods of noise reduction for trains and summarize the progress in current research and the limitations of train body panels and railroad sound barrier technology. Finally, the article introduces the development and potential future applications of acoustic metamaterials and proposes application scenarios of acoustic metamaterials for the specific needs of railroad sound barriers and train car bodies. This synopsis provides a useful platform for researchers and engineers to cope with problems of future high-speed rail noise in the future.
High-speed train is a large-scale electromechanical coupling equipment with a complex structure, where the coupling is interlaced between various system components, and the excitation sources are ...complex and diverse. Therefore, reliability has become the top priority for the safe operation of high-speed trains. As the operating mileage of high-speed trains increases, various key systems experience various degrees of performance degradation and damage failures. Moreover, it is accompanied by the influence of external environmental high interference noise and weak early fault information. Thus, those factors are serious challenges for the condition monitoring and fault diagnosis of high-speed trains. Therefore, this paper summarizes the research progress and theoretical results of the fault detection, fault isolation, and fault diagnosis methods of the key systems of high-speed trains. Finally, the paper summarizes the applicability of the main methods, discusses the challenges and opportunities of condition monitoring and fault diagnosis of high-speed trains, and looks forward to improving its diagnosis level.
Elevated ozone (O
3
) modulates phytohormone signals, which subsequently alters the interaction between plants and herbivorous insects. It has been reported that elevated O
3
activates the plant ...abscisic acid (ABA) signaling pathway, but its cascading effect on the performance of herbivorous insects remains unclear. Here, we used the ABA-deficient tomato mutant
notabilis
(
not
) and its wild type, Ailsa Craig (AC), to determine the role of ABA signaling in mediating the effects of elevated O
3
on
Bemisia tabaci
in field open-top chambers (OTCs). Our results showed that the population abundance and the total phloem-feeding duration of
B. tabaci
were decreased by O
3
exposure in AC plants compared with
not
plants. Moreover, elevated O
3
and
B. tabaci
infestation activated the ABA signaling pathway and enhanced callose deposition in AC plants but had little effect on those in
not
plants. The exogenous application of a callose synthesis inhibitor (2-DDG) neutralized O
3
-induced resistance to
B. tabaci
, and the application of ABA enhanced callose deposition and exacerbated the negative effects of elevated O
3
on
B. tabaci
. However, the application of 2-DDG counteracted the negative effects of O
3
exposure on
B. tabaci
in ABA-treated AC plants. Collectively, this study revealed that callose deposition, which relied on the ABA signaling pathway, was an effective O
3
-induced priming defense of tomato plants against
B. tabaci
infestation.
Mitchell syndrome (MITCH) is a rare autosomal dominant hereditary disorder, characterized by episodic demyelination, sensorimotor polyneuropathy and hearing loss. MITCH is caused by heterozygous ...mutation in the ACOX1 gene, which encodes straight-chain acyl-CoA oxidase, on chromosome 17q25.1. Only 5 unrelated patients have been reported so far, and no reports from China. Here, we describe the first MITCH case in a Chinese individual.
A 7-year-old girl initially presented with diffuse desquamatory rash at age 3. Her clinical symptoms in order of presentation were diffuse desquamatory rash, gait instability, ptosis with photophobia, hearing loss, abdominal pain, diarrhea, nausea, and dysuria. Genetic analysis demonstrated that the patient carried a heterozygous variant c.710A>G(p.Asp237Ser) in the ACOX1 gene, which can cause MITCH symptoms. This is the first MITCH case with gastrointestinal and urinary tract symptoms. After administrating N acetylcysteine amide (NACA), some symptoms were relieved and the patient's condition improved.
This is the first MITCH case in the Chinese population, and we expanded the genotype spectrum of it. The p.Asp237Ser may be a mutational hotspot in ACOX1 regardless of race. In terms of diagnosis, patients with recurrent rash, gait instability, and hearing loss with some autonomic symptoms should raise the suspicion of MITCH and proper and prompt treatment should be given.
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