Our study aimed to investigate whether CAF (cancer-associated fibroblasts) were involved in long noncoding RNAs (lncRNA)-regulated radioresponse in esophageal squamous cell carcinoma (ESCC).
By use ...of lncRNAs PCR array, 38 lncRNAs were screened in esophageal cancer cells and in normal esophageal epithelial cells Het-1A. LncRNA DNM3OS was detected in tumor tissues of patients with ESCC and in matched normal esophageal epithelial tissues by qRT-PCR analysis and
hybridization assay. The association of DNM3OS and tumor radioresistance was investigated
and
. The influences of DNM3OS on DNA damage response (DDR) was investigated by Western blotting, immunofluorescence imaging, and comet assay. The mechanisms by which CAFs promoted DNM3OS expression was investigated by kinase inhibitors' screening, luciferase assay, and chromatin immunoprecipitation.
Among the 38 lncRNAs tested, DNM3OS was found to have a much higher expression level in esophageal cancer cells than in Het-1A. In tumor tissues of 16 patients with ESCC, the expression level of DNM3OS showed an average increase of 6.3429-fold compared with that in matched normal tissues. DNM3OS conferred significant radioresistance
and
by regulating DDR. CAFs promoted the expression of DNM3OS with a 39.2554-fold and 38.3163-fold increase in KYSE-30 and KYSE-140, respectively. CAFs promoted the expression of DNM3OS in a PDGFβ/PDGFRβ/FOXO1 signaling pathway-dependent manner. FOXO1, a transcription factor downstream of PDGFβ/PDGFRβ signaling pathway, initiated the transcription of DNM3OS by binding to DNM3OS promoter.
Our study highlighted CAF-promoted DNM3OS as an attractive target to reverse tumor radioresistance in ESCC.
A new kind of organic precursor, Ti-DHBA-Si (DHBA=3,5-dihydroxybenzoic acid), was prepared via the reaction of a carboxylic group with titanium alkoxide and the modification of the double hydroxyl ...groups with the coupling agent 3-(triethoxysilyl)-propyl isocyanate (TEPIC). The organic precursor Ti-DHBA-Si and polymer PMMA (PMMA=Polymethyl methacrylate) were then simultaneously coordinated with europium ion, and the final polymeric hybrid material, Eu(Ti-DHBA-Si)3PMMA, was obtained after hydrolysis and co-polycondensation with tetraethoxysilane via a sol–gel process. X-ray diffraction patterns reveal that the hybrid material is amorphous. Scanning electronic microscopy images show uniform microstructure without phase separation. Photoluminescent spectra were characterized, and the results show that the hybrid material exhibits strong red-colored fluorescence, which can be explained by intramolecular energy transfer caused by coordination of the organic counterpart.
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•The lanthanide hybrid materials were assembled via a novel method.•Lanthanide complex was covalently bonded to composite matrices of Si and Ti.•Polymer was introduced to lanthanide organic–inorganic hybrid material.•The hybrid material exhibited strong red photoluminescence and high quantum effciency.•The hybrid material possess regular microsphere structure.
Amorphous nickel powder (Ni(0)) was utilised as a catalyst under mild, aqueous, basic conditions for enhancing the sodium borohydride-mediated reduction of C-N multiple bonds such as oximes, imines, ...hydrazones and nitriles to produce the corresponding amines in good to excellent yields.
This paper describes an efficient procedure for the synthesis of micromide, a natural product that shows anti‐solid‐tumor activity. Our strategy involved the synthesis of N‐nosyl‐protected amino ...acids and their N‐methylation with iodomethane. The hindered oligopeptides containing N‐methyl amino acids were synthesized in excellent yields and high purities.
An efficient method to synthesize micromide, a natural product with anti‐solid‐tumor activity, is described. Our strategy involved the synthesis of N‐nosyl‐protected amino acids and their N‐methylation using iodomethane. The hindered oligopeptides containing N‐methyl‐substituted amino acids were synthesized in excellent yields and high purities.
A novel covalently bonded Eu3＋-based silica hybrid material was designed and its spectrophotometric anion sensing property was studied. The fluorescent receptor （europium complex） was covalently ...grafted to the silica matrix via a sol-gel approach. FTIR, UV-vis spectra, scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, X-ray diffraction （XRD） and photoluminescent spectra were characterized, and the results revealed that the hybrid material with nanosphere structure displayed excellent photophysical property. In addition, the selective anion sensing property of the hybrid material was studied by UV-vis and fluorescence spectra. The results showed that the hybrid material exhibited a smart response with fluoride anions.
Mucormycosis is a fatal invasive fungal infection that commonly affects immunocompromised children. The aim of our study was to investigate the clinical manifestations, treatments, and prognosis of ...pediatric patients with mucormycosis.
We conducted a retrospective search in Shenzhen Children's Hospital from July 2013 to July 2023 for all patients with mucormycosis. The clinical manifestation, pathogen detection, radiology, treatments, and prognosis were analyzed.
Four cases were identified. Underlying conditions included acute myeloid leukemia with myeloid sarcoma (n = 1), thalassemia (post-allogeneic hematopoietic stem cell transplantation; n = 1), systemic lupus erythematosus (n = 1), and bilateral nephroblastoma (post-bilateral nephrectomy; n = 1). Two patients were disseminated mucormycosis, one case was pulmonary mucormycosis, and one case was cerebral mucormycosis. Fever, cough, and dyspnea were the main clinical symptoms of pulmonary mucormycosis, headache was the main clinical symptom of cerebral mucormycosis. Lung CT findings included consolidation, multiple nodules, halo sign, air crescent sign, and pleural effusion. The contrast-enhanced CT showed pulmonary artery and pulmonary vein occlusions in two patients and pseudoaneurysm in two patients. Amphotericin B formulations were administered as first-line therapy in all cases; in three cases, Triazole was administered in combination with amphotericin B.
Mucormycosis is a life-threatening disease involving multiple systems. Aorta pseudoaneurysm is a rare and fatal complication, enhanced CT can assist in diagnosis. Early diagnosis and appropriate therapeutic strategies are needed.
UHMWPE granule could induce macrophages and inflammatory responses in interfacial tissues, which eliminated the wear debris of UHMWPE component and further induced dissolution of the surrounding ...bone, leading aseptic loosening. However, the mechanism of synovial cells, especially fibroblast-like synovial (FLS) cells response to UHMWPE, remains unknown. Herein we choose FLS cells as research object. Vimentin (+) CD68 (-) was identified by flow cytometry and immunofluorescent staining assay, and the cells were identified as FLS cells, which was consistent with the experimental requirements. The inhibitory evaluation showed that UHMWPE could significantly promote the proliferation and inhibit apoptosis of FLS cells in dose- and time-dependent manners and increase the levels of proinflammatory cytokines, including IL-6, IL-1β, TNF-α, PGE2, MMP2, and LOX. UHMWPE also can induce the expression of mIL-6R protein in FLS cells and further investigate the relationship between apoptosis and inflammation. Interestingly enough, when we added the interleukin-6 receptor antagonist (IL-6RA), the expression levels of proapoptosis-related proteins increased; in other words, UHMWPE-induced antiapoptosis diminished by IL-6RA (50 μg/ml). Taken together, these findings clearly demonstrated that UHMWPE promote growth in FLS cells through upregulating inflammatory factors to produce antiapoptotic effect.
A new type of combined heterogeneous catalyst, PVP-PdCl
2-CuCl
2/SiO
2-PEG600, was prepared by reaction of silica gel functionalized by polyethylene glycol (PEG) with average molecular weight 600 as ...a carrier system with phase transfer properties, with polyvinylpyrrolidone (PVP)-anchored PdCl
2 and CuCl
2 complex, and characterized by IR, XPS and TEM. The catalyst, in which the Pd/Cu molar ratio is 2/1, has shown good activity and selectivity for dechlorination of aromatic chlorides in water. In the presence of HCOONa as hydrogen donor and basic media, conversion in the dechlorination of
p-chlorotoluene can reach 96.6% at 80°C only for 2.5
h.
Two sugar functionalized naphthalimide derivatives (S1, S2) self-assembled into organogels by a heating-cooling process or triggered by ultrasound. The gelation properties in organic solvents were ...examined by several experiments including UV-vis, fluorescence, FT-IR spectra and SEM, XRD techniques. It was deduced that minor changes in the terminal group had great impact on the gelation and ultrasound responsive properties. Moreover, ultrasound triggered the formation of a yellow emissive gel (S1, with lifetimes in the range of ns) that was readily doped with Eu3+in situ affording luminescent gels with red emission color (with lifetimes in the range of mu s), which expressed efficient energy transfer from the S1 assembly to Eu3+ ion. It was presented that the efficient energy transfer only happened in the ordered fibrous aggregates of S1, whereas, the ET process was not observed in the solution state, indicating the phase control on the ET process. Such findings would pave a new way for the construction of novel rare earth based luminescent materials.
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