We sought to identify biochemical predictors that indicate susceptibility to in-stent restenosis (ISR) after coronary artery bare-metal stenting.
A total of 111 consecutive patients with ...post-percutaneous coronary intervention (PCI) in-stent restenosis of a target lesion within 12 months were matched for age, sex, vessel diameter, and diabetes with 111 controls without post-PCI ISR. Plasma or serum levels of biochemical markers were measured: matrix metalloproteinases (MMP) 2, 3, 9; myeloperoxidase (MPO); asymmetric dimethylarginine (ADMA); lipoprotein (a) (Lpa); apolipoproteins E and D (ApoE and D); and lecitin-cholesterol acyltransferase (LCAT). Multivariable logistic regression association tests were performed.
Increased plasma MMP-3 (OR: 1.013; 95% CI: 1.004-1.023; P = 0.005), MMP-9 (OR: 1.014; 95% CI: 1.008-1.020; P < 0.0001) or MPO (OR: 1,003; 95% CI: 1.001-1.005; P = 0.002) was significantly associated with increased risk of ISR. Increased levels of ADMA (OR: 0.212; 95% CI: 0.054-0.827; P = 0.026), ApoE (OR: 0.924; 95% CI: 0.899-0.951; P < 0.0001), ApoD (OR: 0.919; 95% CI: 0.880-0.959; P = 0.0001), or LCAT (OR: 0.927; 95% CI: 0.902-0.952; P < 0.0001) was associated with risk reduction. No correlation was found between plasma MMP-2 or Lp (a) and ISR risk.
Increased levels of MMP-3, MMP-9, and MPO represent predictors of ISR after bare-metal stent implantation. In contrast, increased ADMA, LCAT, and Apo E and D indicate a decreased in-stent restenosis occurrence.
High density lipoprotein (HDL) decreases in diabetic women more rapidly than what is observed in diabetic men. Here we aimed to study serum LCAT levels and LCAT activity in men and women with type 2 ...diabetes (T2DM) as well as healthy controls.
We measured serum LCAT activity and levels in 40 patients with T2DM plus 40 age–sex and body mass index (BMI)-matched controls. The correlation between LCAT levels and activity was measured in the studied groups, stratified according to gender.
Patients had a lower LCAT activity, diastolic blood pressure, fasting blood sugar, triglyceride, low density lipoprotein cholesterol and a higher LCAT levels than controls. The lower LCAT activity in patients with T2DM was significant after multiple adjustments for age, LCAT levels and BMI, using general linear model (67.9±1.8 vs. 86.5±1.8; P<0.001). Women with T2DM had a lower LCAT activity and a higher LCAT and HDL levels compared to men. The lower LCAT activity in women remained significant after multiple adjustments for age, BMI and LCAT levels; (61.9±3.1 vs. 74.17±3.61; P<0.05). Statin treatment did not have any significant value on the results. HDL was not correlated with LCAT levels or activity in any of the studied populations.
We showed that while LCAT activity is decreased in patients with T2DM, LCAT levels is increased. Patients with T2DM exhibit opposing effects on LCAT activity and LCAT production which is more severe in women. Future prospective studies may elucidate the underlying pathways for these observations.
Lecitin cholesterol acyltransferase (LCAT) deficiency comprises a group of rare disorders related to HDL metabolism. These disorders are characterized by ophthalmologic, hematologic, and renal ...findings. Case diagnosis/treatment: A 15-year-old female who presented with nephrotic syndrome and hypertension was diagnosed with LCAT deficiency by renal biopsy and LCAT enzyme activity. Her edema and hypertension improved with diuretic and antihypertensive therapies. Continued care of her LCAT deficiency is ongoing.
Although rare, LCAT deficiency should be in the differential diagnosis of nephrotic syndrome in the setting of abnormally low HDL cholesterol levels.
Tribological properties of a base oil (poly alpha olefine with 20 wt% deuterated n-hexadecane) were evaluated with a ball-on-flat type tribotest. The addition effect of a lecithin-modified clay to ...the base oil was studied. The presence of deuterium species inside the steel disk after friction tests was investigated by a TOF-SIMS analysis. Deuterium species were observed inside the steel disk with 60 nm depth after friction with the base oil for 60 min. On the other hand, deuterium species were not observed inside the steel disk with 20 nm depth after friction with the oil containing 12 wt% of the lecithin-modified clay. The lecithin-modified clay could be used as a valuable additive for lubricants with hydrogen barrier properties.
U ovom su radu istraživani učinci žutanjka jajeta i/ili lecitina u razjeđivačima sjemena na kakvoću smrznute-odmrznute sperme nerasta. Uključeno je ukupno 19 ejakulata od 8 nerasta pasmine Duroc. ...Svaki je ejakulat bio raspodijeljen i zamrznut u 3 različita razrijeđivača, koji su sadržavali dodatak žutanjka jajeta (20,0 %) (skupina I), lecitina (6,0 %) (skupina II) i kombinaciju žutanjka jajeta (10,0 %) i lecitin (3,0 %) (skupina III). Nakon odmrzavanja vrednovane su: pokretljivost spermija, karakteristike gibljivosti, vijabilnost, integritet akrosome, permeabilnost membrane spermija i aktivnost mitohondrija pomoću računalno podržanog sustava za analizu sperme (CASA), SYBR-14/ etidiumhomodimer-1, FITC-PNA, JC-1 bojenja i HOS testa. Po odmrzavanju, pokretljivost spermija u skupinama I i III nisu se značajno
razlikovale (P>0,05), ali su oba razrijeđivača bili bolji od skupine II (P<0,05). Karakteristike gibljivosti, uključujući pravocrtnu brzinu (VSL), linearnost (LIN) i koeficijent kolebljivosti (WOB), bili su viši u skupinama I i III u odnosu na skupinu II (P<0,05). Isto tako, vijabilnost spermija, permeabilnost membrane i aktivnost mitohondrija bili su viši u skupinama I i III u odnosu na skupinu II (P<0,05). Zaključno, uporaba lecitina bez žutanjka jajeta u razrjeđivaču za krioprezervaciju sjemena nerasta pogoršala je kvalitete zamrznuto-odmrznutog sjemena. Stoga se preporučuje uporaba 20,0 % žutanjka jajeta ili kombinacije 10,0 % žutanjka jajeta i 3,0 % lecitina kao dodataka razrijeđivaču za duboko smrzavanje sjemena nerasta.
Atheroselorosis cardiovascular disease is the leading cause of death in industrial societies. For coronary heart disease, hypercholeterolemia and dyslipoproteinemia are the major risk factors. Low ...serum levels of cholesterol in the HDL fraction is the most common abnormality found in patients with confirmed coronary artery disease. A therapeutical strategy consists in increasing the serum HDL, cholesterol concentration in order to improve the ‘reverse cholesterol transport’. Studies in transgenic mice and rabbits for human apo A-I or human lecithin cholesterol acyl-transferase showed that overexpression of these proteins increases serum HDL cholesterol concentration and reduces diet induced atherogenesis. Furthermore, adenovirus-mediated transfer of human apo A-I and LCAT genes in mice also increases circulating apo A-I and LCAT. Apo A-I and LCAT are two potential targets for gene therapy of patients with atherosclerosis associated with a low HDL cholesterol level.
To investigate the deterioration of fats and oils by hot plate heating, several experiments were carrid out. Lard (2g, 10g) and /or vegetable oils (2g, 10g) kept in beaker (500mL) were heated using ...hot plate for 10min at 200°C. Lard and vegetable oils with or without silicone oil (5 ppm) were also heated using the same way. The effect of some antioxidants such as tocopherol, silicone oil and lecithin on the thermal deterioration was compared. To prevent the deterioration of lard during heating, adding a mixture of tocopherol (0.1%), soybean lecithin (0.5%) and silicone oil (5ppm) showed better effect than adding them separately. Addition of silicone oil (5ppm) alone could supperss the deterioration of fats and oils by heating, residual tocopherol increased. Silicone oil effectively suppress the thermal deterioration, but no such effect was noticed on autoxidation of oils. Commercially available cooking oils with lecithin showed preventive effect on deterioration of oils by heating. The degree of thermal decomposition of tocopherol in heated oils at 200°C was found to much more high levels of oleic acid in sufflower oil (HOSO) than high levels of linoleic acid in safflower oil (HLSO). Deterioration of oils was affected remarkably to the specific surface area and heating temperature.
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