A novel actinobacterial strain, designated AGMB00827
T
, was isolated from swine faeces. Strain AGMB00827
T
was obligately anaerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped ...bacterium. Comparative analyses based on the 16S rRNA gene and whole genome sequence revealed that strain AGMB00827
T
was affiliated to the genus
Collinsella
, and was most closely related to
Collinsella vaginalis
Marseille-P2666
T
(= KCTC 25056
T
). Biochemical analysis showed strain AGMB00827
T
was negative for catalase and oxidase. Interestingly, strain AGMB00827
T
possessed urease activity, which was determined by traditional methods (API test and Christensen’s urea medium), unlike related strains. Furthermore, the major cellular fatty acids (> 10%) of the isolate were C
18:1
ω
9
c
, C
16:0
, C
16:0
DMA and C
18:2
ω
9,12
c
DMA. Based on the whole genome sequence analysis, the DNA G + C content of strain AGMB00827
T
was 52.3%, and the genome size and numbers of rRNA and tRNA genes were 1,945,251 bp, 3 and 46, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain AGMB00827
T
and
C. vaginalis
KCTC 25056
T
were 71.0 and 23.2%, respectively. Additionally, the genome analysis revealed that strain AGMB00827
T
possesses urease gene cluster including
ureABC
and
ureDEFG
while the related strains do not have those genes, which is consistent with the urease activity. On the basis of polyphasic taxonomic approach, strain AGMB00827
T
represents a novel species within the genus
Collinsella
, for which the name
Collinsella urealyticum
sp. nov. is proposed. The type strain is AGMB00827
T
(= KCTC 25287
T
= GDMCC 1.2724
T
).
Escherichia coli normally colonizes the lower intestine of animals and humans, but some serotypes are foodborne pathogens. The Escherichia coli K_EC180 was isolated from swine feces that were ...collected from a weaner pig. In this genome announcement, E. coli K_EC180 was sequenced using PacBio RS II and Illumina NextSeq 500 platforms. The complete chromosome of E. coli K_EC180 is composed of one circular chromosome (5,017,281 bp) with 50.4% of guanine + cytosine (G + C) content, 4,935 of coding sequence (CDS), 88 of tRNA, and 22 of rRNA genes. The complete genome of E. coli K_EC180 contains the toxin genes such as shiga-like toxins (stxA and stxB).
An obligately anaerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped strain AGMB00832
T
was isolated from swine faeces. Phylogenetic analysis based on the 16S rRNA gene, ...together with the housekeeping genes,
gyrB
and
rpoD
, revealed that strain AGMB00832
T
belonged to the genus
Faecalicatena
and was most closely related to
Faecalicatena orotica
KCTC 15331
T
. In biochemical analysis, strain AGMB00832
T
was shown to be negative for catalase, oxidase and urease. Furthermore, the isolate was positive for β-glucosidase, β-glucuronidase, glutamic acid decarboxylase, proline arylamidase, acid phosphatase and naphthol-AS-BI-phosphohydrolase. The major cellular fatty acids (> 10%) of the isolate were C
14:0
, C
16:0
and C
18:1
ω11t DMA. Based on the whole genome sequence analysis, the DNA G + C content of strain AGMB00832
T
was 44.2 mol%, and the genome size and numbers of rRNA and tRNA genes were 5,175,159 bp, 11 and 53, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain AGMB00832
T
and related strains were ≤ 77.4 and 22.5%, respectively. Furthermore, the genome analysis revealed the presence of genes for alkaline shock protein 23 and cation/proton antiporters, which may facilitate growth of strain AGMB00832
T
in alkaline culture condition. On the basis of polyphasic taxonomic approach, strain AGMB00832
T
represents a novel species within the genus
Faecalicatena
, for which the name
Faecalicatena faecalis
sp. nov. is proposed. The type strain is AGMB00832
T
(= KCTC 15946
T
= NBRC 114613
T
).
Antimicrobial resistance (AMR) is a worldwide health problem affecting humans, animals, and the environment within the framework of the “One Health” concept. The aim of our study was to evaluate the ...prevalence of pathogenic strains of the species Escherichia coli (E. coli), their AMR profile, and biofilm-forming potential. The isolated strains from three swine faeces and free lagoons (ISO 16654:2001/Amd 1:2017) were confirmed using Phoenix M50 and 16S rDNA PCR. The antibiotic sensitivity to 34 clinically applied antibiotics was determined by Phoenix M50 and the disc diffusion method, according to the protocols of the CLSI and EUCAST. We confirmed the presence of 16 E. coli isolates, of which 87.5% were multi-drug-resistant and 31.25% performed strong biofilms. The possibility for the carrying and transmission of antibiotic-resistance genes to quinolones (qnr), aminoglycosides (aac(3)), β-lactamase-producing plasmid genes ampC, and blaSHV/blaTEM was investigated. We confirmed the carrying of blaSHV/blaTEM in one and ampC in seven isolates. The strains were negative for the virulence genes (ETEC (LT, STa, and F4), EPEC (eae), and STEC/VTEC (stx and stx2all)). The results should contribute to the development of effective measures for limitation and control on the use of antibiotics, which is a key point in the WHO action plan.
Odour emanating from anaerobic lagoons and swine production facilities has increased the tension among rural neighbors and among urban and rural residents. Storage of swine manure is associated with ...the production of a variety of odorous compounds, including ammonia, organic acids and alcohols, and sulphides. Although the generation of these chemicals is the result of microbiological activity, little is known about the types of microorganisms responsible for their production. We have initiated an approach to determine and compare the bacterial populations present in both pig faeces and manure storage pits. Total DNA was isolated from both of these ecosystems. DNA sequence analyses of PCR amplified 16S rDNA genes derived from eubacterial sequences were carried out. Similarity analyses of the 16S sequences indicated the presence of primarily low G + C Gram-positive bacteria, such as Clostridium sp., Streptococcus sp., and Lactobacillus sp. in both ecosystems. Many of the sequences were from unidentified microorganisms. These results indicate that the primary eubacteria identified in swine faeces and manure pits are low G + C, Gram-positive bacteria.
In order to incinerate the swine feces, which causes subterranean water pollution in the Miyakonojyo basin, low quality charcoal was used as auxiliary fuel. Through this method, swine feces which do ...not self-burn can be converted into an energy source. From the viewpoint of the energy yield, it is preferable that the processing temperature of the charcoal is less than 300°C. There are some advantages in mixing swine feces and charcoal ; the mixture produces sufficient heat energy for self-burning, the bad odor of feces decreases, the drying of feces becomes easier and faster, and the handling of feces is improved. A technical scale experiment for the continuous incineration of the feces-charcoal mixture was done for 48 hours. During the experiment, the amount of steam generated from the system was measured. The result revealed that the quantity of the steam is insufficient for high-temperature kiln-drying system such as 120°C, but would be useful for a mild-temperature kiln-drying system. Because the energy efficiency is quite high, it is expected that the direct burning of wood biomass, though it is low-technology, will contribute to the large saving of fossil fuel.
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