Izolacija in identifikacija spojine z antimutagenim učinkom iz polstene mlečnice (Lactarius vellereus (fries) fries) = Isolation and identification of a compound with antimutagenic activity from fleecy milk-cap (Lactarius vellereus (fries) fries) : diplomska naloga
Dmitrović, Jasmina
Na osnovi preizkusanj izvleèkov glive Lactarius vellereus smo ugotovili, da doloèene frakcije delujejo antimutageno, zato smo se odloèili za nadaljnje preizkusanje izvleèkov te glive. Z uporabo ...kolonske kromatografije smo uspeli aktivno frakcijo st. 5 loèiti na podfrakcije. Delo je potekalo vzporedno z analizami na Nacionalnem institutu za biologijo, Oddelek za genetsko toksikologijo in molekularno biologijo raka, kjer so preverjali antimutageno aktivnost nasih vzorcev s testom povratnih mutacij z metabolno aktivacijo. Najbolj èisto izolirano podfrakcijo, pri kateri je bilo opaziti na plosèi za tankoplastno kromatografijo samo eno liso, smo analizirali z jedrsko magnetno resonanco. Po primerjavi spektra s spektrom standarda, lahko za izolirano spojino, ki deluje antimutageno, z veliko verjetnostjo trdimo, da gre za oleinsko kislino. Poleg frakcije, ki smo jo loèili na podfrakcije, je se frakcija st. 4 delovala moèno antimutageno, zato bi bilo smiselno z nadaljnim loèevanjem izolirati se druge uèinkovine, ki morda delujejo se moèneje kot oleinska kislina.
Type of material - undergraduate thesis
Publication and manufacture - Ljubljana : [Dmitrović J.], 2003
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