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Morfometrične in funkcionalne lastnosti granuloza-luteinskih celic v pogojih naravnega in spodbujenega ciklusa = Morphometric and functional characteristics of human granulosa-luteal cells in natural and stimulated IVF-ET cycles : doktorska disertacijaGeršak, KsenijaObjective: to investigate human granulosa-luteal cells derived from natural and stimulated IVF-ET cycles. Setting: IVF unit, Department of Obstetrics and Gynaecology, Medicl Centre Ljubljana. ... Material in methods: Cells were obtained from dominant follicles of 52 women with natural cycles in which preovulatory hCG was given when the follicle was mature, and from 50 folliclesof 45 women undergoing IVF-ET with hMG and hCG. Subpopulations were observed by computerized image analysis using HE and immuperoxidase staining for hCG localization. Hormone assays were done on all follicular Fluids for hCG, estradiol and progesterone. ResultsČ Four subpopulations were larger thanthose from the stimulated ones. In luteinized cell types, there was no difference in cell area between natural and stimulated cycles. No differences were detected in the relative number of each of the four subpopulations withinthe follicular aspirates from naturally developing follicles. In stimulated ones, follicles with fertilized oocytes had more nonluteinized cells and fewer large luteinized cells. Three staining types of hCG localization were foud: on the surface membrane, on the surface membrane and within the cytoplasm, and within the cytoplasm alone. The hCG stained cells from natural cycles were larger than the ones from stimulated cycles. The natural developing follicles contained more hCG stained cells than the stimulated ones. The follicles with fertilizable oocytes had more cells with cytoplasmic hCG localization regardless type of IVF-ET protocol. Only in natural cycles there was a correlation between follicular fluid hCG levels andthe percentage of the hCG stained cells. Human chorionic gonadotropin levels in natural cycles were also influenced by fertilizability of oocytesČ in follicles with fertilized oocytes, follicular fluid had higher hCG levels than with nonfertilized oocytes. Conclusion: We found the differences in morphometric charaster.Type of material - dissertation ; adult, seriousPublication and manufacture - Ljubljana : [K. Geršak], 1996Language - slovenianCOBISS.SI-ID - 58084864
Author
Geršak, Ksenija
Other authors
Tomaževič, Tomaž
Topics
Granulosa cells |
Cytology |
Granulosa cells |
Metabolim |
Menstrual cycle |
Physiology |
Menstrual cycle |
Drug effects |
Granuloza celice |
Citologija |
Granuloza celice |
Metabolizem |
Menstrualni ciklus |
Fiziologija |
Menstrualni ciklus |
Učinki zdravil |
umetna oploditev |
zunajtelesna oploditev |
jajčniki |
rumeno telesce |
luteinske celice |
morfologija |
lastnosti |
disertacije |
corpus luteum GLEJ: rumeno telesce
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Library | Call number – location, accession no. ... | Copy status |
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National and University Library, Ljubljana | GS II 470848 glavno skladišče | available - reading room |
MF, Central Medical Library, Lj. | doktorske disertacije GERŠAK Ksenija Morfometrične IN: 099700609 |
available - outside loan, loan period: 1 months |
MF, Central Medical Library, Lj. | doktorske disertacije GERŠAK Ksenija Morfometrične IN: 099700608 |
available - outside loan, loan period: 1 months |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Geršak, Ksenija | 00814 |
Tomaževič, Tomaž | 07007 |
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