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  • Identification of prosthetic joint infections with 16S amplicon metagenomic sequencing : comparison with standard cultivation approach
    Mahnič, Aleksander ...
    Prosthetic joint infections (PJIs) are commonly diagnosed via culture-based methods, which may miss hard-to-grow pathogens. This study contrasts amplicon metagenomic sequencing (16S AS) with ... traditional culture techniques for enhanced clinical decision-making. We analyzed sonicate fluid from 27 patients undergoing revision arthroplasty using both methods, emphasizing the distinction between contaminants and true positives. Our findings show moderate agreement between the two methods, with a Cohen's kappa of 0.490, varying across bacterial genera (Cohen's kappa -0.059 to 1). The sensitivity of 16S AS compared to culture was 81% (95% CI, 68% to 94%). Sequencing revealed greater microbial diversity, including anaerobic genera like Anaerococcus and Citrobacter. Interestingly, several culture-negative PJI samples showed diverse bacteria via 16S AS. Despite rigorous controls and algorithms to eliminate contaminants, confirming bacteria presence with 16S AS remains a challenge. This highlights the need for improved PJI diagnostic methods, while also pointing out the limitations of next-generation sequencing (NGS) as a clinical diagnostic tool.
    Source: Diagnostic Microbiology and Infectious Disease. - ISSN 0732-8893 (Vol. 109, iss. 1, art. 116188, 2024, str. 1-5)
    Type of material - article, component part ; adult, serious
    Publish date - 2024
    Language - english
    COBISS.SI-ID - 184967427

    Link(s):

    https://www.sciencedirect.com/science/article/pii/S0732889324000178?via%3Dihub
    https://doi.org/10.1016/j.diagmicrobio.2024.116188
    DOI

source: Diagnostic Microbiology and Infectious Disease. - ISSN 0732-8893 (Vol. 109, iss. 1, art. 116188, 2024, str. 1-5)

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