E-resources
Peer reviewed
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Wang, Jin-Hong; Yang, Ming; Fan, Dong-Mei; Xu, Yuan-Sheng; Xiong, Dong-Sheng; Yang, Chun-Zheng
Zhōngguó yīxué kēxuéyuàn xuébào 30, Issue: 5Journal Article
To establish a three-step purification method of preparative-scale antiCD20 (Fab')2 using AKTA prime. AntiCD20 (Fab')2 was extracted by hyperosmotic solution and then purified by CM sepharose FF, phenyl sepharose FF, and protein G sepharose FF. Around 8 mg anti-CD20 (Fab')2, whose purification was 96.678%, was purified. The antigen-binding activity of antiCD20 (Fab')2 was similar to that of antiCD20 (Fab')2 purified by protein G sepharose FF and S-100. The three-step purification method can obtain high-purity preparative-scale antiCD20 (Fab')2 in a simple way.
