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朱闰月 赵志勇 杨宪立 聂冬霞 徐斐 武爱波 宋素泉
分析化学, 2015, Volume: 43, Issue: 7Journal Article
采用基质固相分散( Matrix solid phase dispersion, MSPD)技术,结合高效液相色谱-电喷雾离子化串联质谱联用技术( HPLC-ESI-MS/MS)分析,在多反应监测( MRM)模式下建立了鸡蛋中呕吐毒素、黄曲霉素、玉米赤霉烯酮等15种真菌毒素生物标志物的同时定量分析的方法。样品与吸附剂C18颗粒直接混合装柱,并用20 mL 1 mmol/L甲酸铵的乙腈/甲醇(1:1, V/V)溶液洗脱、吹干,萃取液经复溶、过滤后即可进行检测,整个分析过程无需复杂的净化、离心等步骤。本方法在0.2-100 ng/mL范围内具有良好的线性关系,相关系数R2逸0.9931,检出限在0.05-2.0μg/kg 之间,定量限在0.2-4.0μg/kg 之间,样品的提取回收率为61%-90%。
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