The aim of the study was the evaluate the immunomodulating effect of a 1,2,4-triazole derivative liposome emulsion (Trifuzol) in puppies vaccinated against parvovirus enteritis and canine distemper. The used Trifuzol liposome emulsion led to a significant increase in the absolute number of lympho-cytes and their immunoregulatory subpopulations, phagocytic activity of neutrophils. The level of antibodies against parvovirus enteritis (CPV) and canine distemper (CDV) in vaccinated and Trifuzol-treated puppies reached zero values which were statistically significantly lower than those in vac-cinated-only puppies (21.0 U/mL and 27.0 U/mL, respectively). It was concluded that the tested liposome emulsion of Trifuzol had an positive effect on the humoral immunity in puppies and can be used in clinical practice as an immunomodulatory agent.