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Searle, Brian C; Lawrence, Robert T; MacCoss, Michael J; Villén, Judit
Nature methods, 08/2019, Volume: 16, Issue: 8Journal Article
Proteins can be phosphorylated at neighboring sites resulting in different functional states, and studying the regulation of these sites has been challenging. Here we present Thesaurus, a search engine that detects and quantifies phosphopeptide positional isomers from parallel reaction monitoring and data-independent acquisition mass spectrometry experiments. We apply Thesaurus to analyze phosphorylation events in the PI3K/AKT signaling pathway and show neighboring sites with distinct regulation.
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