E-resources
Peer reviewed
Open access
-
Aguilar‐Rojas, Arturo; Castellanos‐Castro, Silvia; Matondo, Mariette; Gianetto, Quentin Giai; Varet, Hugo; Sismeiro, Odile; Legendre, Rachel; Fernandes, Julien; Hardy, David; Coppée, Jean‐Yves; Olivo‐Marin, Jean‐Christophe; Guillen, Nancy
Cellular microbiology, August 2020, Volume: 22, Issue: 8Journal Article
Entamoeba histolytica is the causative agent of amebiasis, an infectious disease targeting the intestine and the liver in humans. Two types of intestinal infection are caused by this parasite: silent infection, which occurs in the majority of cases, and invasive disease, which affects 10% of infected persons. To understand the intestinal pathogenic process, several in vitro models, such as cell cultures, human tissue explants or human intestine xenografts in mice, have been employed. Nevertheless, our knowledge on the early steps of amebic intestinal infection and the molecules involved during human–parasite interaction is scarce, in part due to limitations in the experimental settings. In the present work, we took advantage of tissue engineering approaches to build a three‐dimensional (3D)‐intestinal model that is able to replicate the general characteristics of the human colon. This system consists of an epithelial layer that develops tight and adherens junctions, a mucus layer and a lamina propria‐like compartment made up of collagen containing macrophages and fibroblast. By means of microscopy imaging, omics assays and the evaluation of immune responses, we show a very dynamic interaction between E. histolytica and the 3D‐intestinal model. Our data highlight the importance of several virulence markers occurring in patients or in experimental models, but they also demonstrate the involvement of under described molecules and regulatory factors in the amoebic invasive process. The human colon is home to multiple pathogens and is often the sole target for intestinal diseases. To overcome ethical and methodological limitations of research on intestinal infections, we built a a 3D‐scaffold with cells and collagen reproducing important characteristics of the intestine. This device with other cutting‐edge technologies have made it possible to successfully examine tissue interaction with Entamoeba histolytica, the agent of amebiasis. We highlighted novel regulatory mechanisms used by this parasite to modulate immune responses and survival within the human intestine.
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.