Biological nitrogen (N) fixation (BNF), an important source of N in terrestrial ecosystems, plays a critical role in terrestrial nutrient cycling and net primary productivity. Currently, large uncertainty exists regarding how nutrient availability regulates terrestrial BNF and the drivers responsible for this process. We conducted a global meta‐analysis of terrestrial BNF in response to N, phosphorus (P), and micronutrient (Micro) addition across different biomes (i.e, tropical/subtropical forest, savanna, temperate forest, grassland, boreal forest, and tundra) and explored whether the BNF responses were affected by fertilization regimes (nutrient‐addition rates, duration, and total load) and environmental factors (mean annual temperature MAT, mean annual precipitation MAP, and N deposition). The results showed that N addition inhibited terrestrial BNF (by 19.0% (95% confidence interval CI: 17.7%‒20.3%); hereafter), Micro addition stimulated terrestrial BNF (30.4% 25.7%‒35.3%), and P addition had an inconsistent effect on terrestrial BNF, i.e., inhibiting free‐living N fixation (7.5% 4.4%‒10.6%) and stimulating symbiotic N fixation (85.5% 25.8%‒158.7%). Furthermore, the response ratios (i.e., effect sizes) of BNF to nutrient addition were smaller in low‐latitude (<30°) biomes (8.5%‒36.9%) than in mid‐/high‐latitude (≥30°) biomes (32.9%‒61.3%), and the sensitivity (defined as the absolute value of response ratios) of BNF to nutrients in mid‐/high‐latitude biomes decreased with decreasing latitude (p ≤ 0.009; linear/logarithmic regression models). Fertilization regimes did not affect this phenomenon (p > 0.05), but environmental factors did affect it (p < 0.001) because MAT, MAP, and N deposition accounted for 5%‒14%, 10%‒32%, and 7%‒18% of the variance in the BNF response ratios in cold (MAT < 15°C), low‐rainfall (MAP < 2,500 mm), and low‐N‐deposition (<7 kg ha−1 year−1) biomes, respectively. Overall, our meta‐analysis depicts a global pattern of nutrient impacts on terrestrial BNF and indicates that certain types of global change (i.e., warming, elevated precipitation and N deposition) may reduce the sensitivity of BNF in response to nutrient enrichment in mid‐/high‐latitude biomes. This study addresses how nutrient addition regulates biological nitrogen (N) fixation (BNF) in terrestrial ecosystems and uncovers the latitude patterns and drivers of BNF in response to nutrient enrichment. We found a negative effect of N addition, a positive effect of Micro addition, and an inconsistent effect of P addition on terrestrial BNF and also observed a less sensitivity of BNF to nutrient addition in low‐latitude biomes than in mid‐/high‐latitude biomes. Our findings indicate that certain types of global change (warming, elevated precipitation and N deposition) may reduce the nutrient constraints of BNF in mid‐/high‐latitude biomes.