Many public organizations implement teleworking: an organizational innovation expected to improve the working conditions of public servants. However, it is unclear to what extent teleworking is beneficial for public servants. This study adds to the literature by studying the effects of teleworking on a day-to-day basis. We used a daily diary methodology and followed public servants across five consecutive working days. Studies that apply a daily survey method are more accurate than cross-sectional measures because they reduce recall bias. The results highlight that public servants experience quite negative effects from teleworking, including greater professional isolation and less organizational commitment on the days that they worked entirely from home. Contrary to predictions, working from home did not affect work engagement. We also found that higher leader–member exchange (LMX) reduced the impact of teleworking on professional isolation. These findings not only contribute to the literature by showing the unfavorable effects of teleworking but also highlight that LMX can, to some extent, reduce these negative effects.