Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern

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Shared Mutations in Emerging SARS-CoV-2 Circulating Variants May Lead to Reverse Transcription-PCR (RT-PCR)-Based Misidentification of B.1.351 and P.1 Variants of Concern

Losada, Carmen; Rico-Luna, Carla; Otero-Sobrino, Álvaro; Molero-Salinas, Andrea; Buenestado-Serrano, Sergio; Candela, Ana; Pérez-Lago, Laura; Muñoz, Patricia; Catalán, Pilar; de Viedma, Darío García

Microbiology spectrum, 10/2021, Volume: 9, Issue: 2

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Reverse transcription-PCRs (RT-PCRs) targeting SARS-CoV-2 variant of concern (VOC) mutations have been developed to simplify their tracking. We evaluated an assay targeting E484K/N501Y to identify B.1.351/P1. Whole-genome sequencing (WGS) confirmed only 72 (59.02%) of 122 consecutive RT-PCR P.1/B.1.351 candidates. Prescreening RT-PCRs must target a wider set of mutations, updated from WGS data from emerging variants.

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