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Farrugia, Cher; Stafford, Graham P.; Potempa, Jan; Wilkinson, Robert N.; Chen, Yan; Murdoch, Craig; Widziolek, Magdalena
The FEBS journal, March 2021, Volume: 288, Issue: 5Journal Article
Several studies have unveiled a link between gum disease (periodontitis) and increased risk of cardiovascular disease. It has previously been shown that the oral pathogen Porphyromonas gingivalis (Pg) induces vascular damage in a zebrafish larvae infection model; however, the mechanisms underlying Pg‐induced vascular effects remain unclear. Using in vitro assays and transgenic zebrafish larvae, Craig Murdoch and colleagues show that gingipain, a protease expressed by Pg, cleaves cell surface adhesion molecules that enable endothelial cells to bind to one another, leading to increased vascular permeability. These results provide a compelling mechanism by which oral bacteria could promote cardiovascular disease. Several studies have shown a clear association between periodontal disease and increased risk of cardiovascular disease. Porphyromonas gingivalis (Pg), a key oral pathogen, and its cell surface‐expressed gingipains, induce oedema in a zebrafish larvae infection model although the mechanism of these vascular effects is unknown. Here, we aimed to determine whether Pg‐induced vascular damage is mediated by gingipains. In vitro, human endothelial cells from different vascular beds were invaded by wild‐type (W83) but not gingipain‐deficient (ΔK/R‐ab) Pg. W83 infection resulted in increased endothelial permeability as well as decreased cell surface abundance of endothelial adhesion molecules PECAM‐1 and VE‐cadherin compared to infection with ΔK/R‐ab. In agreement, when transgenic zebrafish larvae expressing fluorescently labelled PECAM‐1 or VE‐cadherin were systemically infected with W83 or ΔK/R‐ab, a significant reduction in adhesion molecule fluorescence was observed specifically in endothelium proximal to W83 bacteria through a gingipain‐dependent mechanism. Furthermore, this was associated with increased vascular permeability in vivo when assessed by dextran leakage microangiography. These data are the first to show that Pg directly mediates vascular damage in vivo by degrading PECAM‐1 and VE‐cadherin. Our data provide a molecular mechanism by which Pg might contribute to cardiovascular disease.
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