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Mitić, Žarko; Cakić, Milorad; Nikolić, Goran M.; Nikolić, Ružica; Nikolić, Goran S.; Pavlović, Radmila; Santaniello, Enzo
Carbohydrate research, 02/2011, Volume: 346, Issue: 3Journal Article
Synthesis, UV–vis, ATR-FTIR, FT-IRIS, EPR spectroscopic and different physicochemical techniques are described for the direct analysis of bioactive copper(II)–pullulan complexes which permits the important anti-hypocupremic and anti-microcitary anemic preparation to be obtained. Bioactive copper(II) complexes with polysaccharides, like pullulan and dextran, are important in both veterinary and human medicine for the treatment of hypochromic microcitary anemia and hypocupremia. In aqueous alkaline solutions, Cu(II) ion forms complexes with the exopolysaccharide pullulan and its reduced low-molecular derivative. The metal content and the solution composition depend on pH, temperature, and time of the reaction. The complexing process begins in a weak alkali solution (pH >7) and involves OH groups of pullulan monomer (glucopyranose) units. Complexes of Cu(II) ion with reduced low-molecular pullulan (RLMP, M w 6000 g mol −1) were synthesized in water solutions, at the boiling temperature and at different pH values ranging from 7.5 to 12. The Cu(II) complex formation with RLMP was analyzed by UV–vis spectrophotometry and other physicochemical methods. Spectroscopic characterizations (ATR-FTIR, FT-IRIS, and EPR) and spectra–structure correlation of Cu(II)–RLMP complexes were also carried out.
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