E-resources
Peer reviewed
-
薛松; 黄高铂; 杨晓飞; 张野; 李彧
临床肝胆病杂志, 2021, Volume: 37, Issue: 10Journal Article
R735.7; 目的 观察血浆IL-6及程序性死亡受体(PD-1)在肝细胞癌(HCC)患者外周血CD8+T淋巴细胞中的表达,评估IL-6对HCC患者CD8+T淋巴细胞中PD-1表达和功能的影响.方法 纳入2019年1月—2019年9月期间在陕西省人民医院或空军军医大学第二附属医院(第四军医大学唐都医院)就诊的HCC患者44例(HCC组),同时纳入年龄和性别匹配的健康对照者19例(HC组),采集外周血,分离血浆和外周血单个核细胞,分选CD8+T淋巴细胞,ELISA法检测血浆IL-6水平,流式细胞术检测PD-1在CD8+T淋巴细胞中的表达水平.使用IL-6中和抗体刺激分选的CD8+T淋巴细胞24 h,CCK-8法检测细胞增殖,ELISA法检测培养上清IFNγ和TNFα水平,实时定量PCR法检测穿孔素、颗粒酶B和颗粒溶素mRNA相对表达量,Western blot法检测STAT3和Src磷酸化水平.计量资料两组间比较采用t检验或配对t检验;计数资料组间比较采用χ2检验.结果 HCC组患者血浆IL-6水平较HC组显著升高(99.67±20.92)pg/mL vs(81.05±16.76)pg/mL,t=3.427,P=0.0011.虽然CD3+CD8+T淋巴细胞比例在HCC组和HC组患者之间的差异无统计学意义(P>0.05),但PD-1+CD8+细胞比例在HCC组患者中显著升高(3.79% ±1.36%vs 2.20% ±0.47%,t=5.335,P<0.0001).使用IL-6中和抗体抑制HCC组患者CD8+T淋巴细胞的IL-6虽不影响细胞增殖,但可降低PD-1表达(2.67% ±0.91%vs 3.33% ±1.12%,t=2.177,P=0.035),增加IFNγ分泌(13.50±3.82)pg/mL vs(10.82±1.37)pg/mL,t=3.170,P=0.0028,穿孔素和颗粒酶B mRNA相对表达量亦显著升高(t值分别为6.161、14.140,P值均<0.0001),同时伴有磷酸化STAT3水平降低(P<0.0001).结论 抗人IL-6中和抗体可能通过增加穿孔素和颗粒酶B水平、增强细胞因子分泌以及抑制PD-1表达增强HCC患者CD8+T淋巴细胞的功能.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.