The problem of road route evaluation in the process of planning and designing is reduced to making a decision regarding route selection which is preceded by a detailed analysis of each potential alternative assessed by experts. The aim of this paper is to show that conclusions regarding the evaluation and selection of the adequate road transport route can be obtained by the application of the multi-criteria analysis. In order to fulfill our research aim, a model which includes criteria and sub-criteria for road route selection is set. The defined model can be applied in the decision-making process related to the road route evaluation and selection. Its value lies in the elaborated methodology of the multi-criteria optimization applied in the transport planning and designing. The defined model was applied in the evaluation and selection of the Jelsane - Postojna highway route by using the PROMETHEE II method for the multi-criteria ranking of alternatives.