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Ricciardi, A; Parente, E; Clementi, F. (Basilicata Univ., Potenza (Italy). Dipartimento di Biologia Difesa e Tecnologie Agroforestali)
Annali di microbiologia ed enzimologia, (1997), Volume: 47Journal Article
Growth and expolysaccharide (EPS) production by Streptococcus thermophilus SY and Lactobacillus delbrueckii subsp. Bulgaricus NCFB 2772 in pure culture or in association were studied during batch fermentations at controlled pH (6.0) in a deproteinized whey-based substrate at 37, 40 and 45 deg C. EPS production was growth-associated for both species. Optimal temperature for EPS production by Str. thermophilus SY was 37 deg C, with a maximum production of 216 mg/l after 8 h of incubation. Optimal temperature for EPS production by Lb. delbrueckii subsp. bulgaricus NCFB 2772 was 40 deg C, with a maximum production of 120 mg/l after 3 h of incubation. Growth in association did not result in an increased EPS production. In mixed cultures, the maximum EPS concentration (220 mg/l) was obtained after 8 h at 40 deg C E' stata studiata la produzione di esopolisaccaridi da parte di Streptococcus thermophilus SY e Lactobacillus delbrueckii subsp. Bulgaricus NCFB 2772, in coltura pura e in associazione, in substrato a base di siero di latte deproteinizzato a pH 6.0, a 37, 40 e 45 gradi C. In tuti i casi la produzione di polisaccaride e' risultata associata alla crescita. La massima produzione da parte di Streptococcus thermophilus SY (216mg/l) e' stata osservata a 37 gradi C, dopo 8 h di fermentazione, mentre per Lb. delbrueckii subsp. bulgaricus NCFB 2772 i livelli piu' elevati di EPS (120 mg/l) sono stati ottenuti a 40 gradi C, dopo 3 h di fermentazione. L'impiego delle due specie in associazione non ha portato un significativo incremento del livello di EPS, risultando la massima produzione pari a 220 mg/l (a 40 gradi C, dopo 8 h di fermentazione)
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