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Xing, Lu-juan; Hu, Ya-ya; Hu, Hong-yan; Ge, Qing-feng; Zhou, Guang-hong; Zhang, Wan-gang
Food chemistry, 03/2016, Volume: 194Journal Article
•The antioxidant activity of XHP was evaluated.•XHP were separated based on molecular weight and polarity differences.•Peptide with the strongest activity had 74.45% scavenging effect on DPPH.•The sequence of main antioxidative peptide was identified as Asp-Leu-Glu-Glu. This study mainly focused on the purification and identification of antioxidative peptides generated in dry-cured Xuanwei ham. Based on scavenging effect on free radicals and ferrous ion, the antioxidant activity of crude peptides from Xuanwei ham was assessed. From the scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH) radicals and superoxide anion (O2−), it was suggested that XHP generated during the ripening period had a strong antioxidant activity. By using size exclusion chromatography, anion exchange column and reversed-phase HPLC, fractions with a strong antioxidative activity were separated based on their molecular weight and polarity differences. The fraction with strong antioxidant effect was further characterized by LC–MS/MS. The results suggest that antioxidative peptides are produced during the long processing of Xuanwei ham among which the tetrapeptide Asp-Leu-Glu-Glu could be one of the main peptides that play key role in the antioxidant activity.
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