The steady-state thermal environment creates obvious challenges to health and energy, which arouse people's concern on the dynamic thermal environment. As an important way to improve thermal comfort and reduce energy consumption, airflow has been widely concerned. Being a highly acceptable airflow, simulating natural wind can be adopted to set up a dynamic thermal environment. Influencing factors of the dynamic thermal environment are much more spread out than the steady-state thermal environment and the human thermal comfort problem gets start from the relatively simple steady-state thermal comfort, those two determiners work and led to the consequence that research on the human comfort model of the dynamic thermal environment is relatively limited. Based on the fuzzy comprehensive evaluation method, this paper establishes the human thermal comfort model of simulating natural wind environment. Combined with the human thermal comfort experiment, the evaluation indexes of Mean Thermal Sensation Vote (MTSV) and Dissatisfaction Rate (DR) are obtained. By comparing the PMV-PPD index and MTSV-DR index with the experimental results of human thermal comfort, it was found that there was a large deviation between the PMV-PPD index and the experimental results, but the MTSV-DR index was in good agreement. According to the range of dissatisfaction rate, the appropriate combination of environmental parameters is recommended. It provides a new way of thinking for human thermal comfort evaluation in dynamic thermal environment. •The simulating natural wind is introduced into the steady-state air-conditioning environment to form a dynamic simulating natural wind environment.•The human thermal comfort model is established by fuzzy comprehensive evaluation, and the MTSV-DR indexes are put forward to evaluate the comfort of the environment.•The MTSV-DR is compared with PMV-PPD and the experimental result to verify the reliability of the MTSV-DR.•Adding simulating natural wind in steady-state air-condition environment can increase the indoor comfortable temperature and achieve the purpose of energy saving.•When the dissatisfaction rate of the population is required to be less than 10% (DR is less than 10%), the MTSV is between -0.05 and +0.05.6)It is recommended that the indoor temperature be 28℃, the turbulence intensity be 0.68, and the blowing time be 5 minutes.