Protozoa of the genus cause the disease giardiosis in a large number of animal species, but also in humans. This disease can be either subclinical or followed by diarrhea, malabsorption, weight loss and stunted growth. The aim of this study was to examine the presence of sp. in different species of animals in Belgrade Zoo (asymptomatic infection) and to compare the sensitivity of parasitological and molecular-genetic methods. Examination of the presence of sp. protozoa was performed in 52 fecal samples, using fecal flotation and molecular-genetic PCR method. No positive samples were detected when deploying the parasitological method, while PRC analysis revealed the presence of sp. in one animal - ring-tailed lemur ( ), which indicates the advantage of the molecular-genetic method over the parasitological one. The absence of sp. in other samples can be explained by adequate housing conditions, successful implementation of hygienic and sanitary measures, as well as good veterinary practice. Use of PCR method for testing the presence of sp. showed a higher sensitivity when compared to the fecal flotation method.