Examination of the internal structure and composition of cultural object is extremely important for its conservation and should be carried out before conservation is planned. Knowledge of the internal structure of an object is also important for the study of ancient manufacturing technologies and sometimes also for determination of its function. In the examination of the interior structure and composition of cultural objects often only methods involving non-destructive examination (NDE) are acceptable. The shape, structure and condition of metal parts can be revealed, e.g. by conventional X-ray Radiography (XR), or computed tomography (CT), while Neutron Radiography (NR) primarily helps to detect the presence of organic materials and in their identification. In the paper some typical examples of the use of NR in the examination of the internal structure of selected cultural objects are presented. The examination of these objects by NDE techniques was an integral part of their conservation process, as well as of their study by archaeologists and historians.