This paper presents the energy and exergy analysis of thermal power plant Tuzla in Tuzla, Bosnia and Herzegovina. The main aim of this paper is to analyze the components of a 200 MW steam power plant unit in order to identify and quantify the sites with the highest exergy losses and to calculate exergy efficiency values of all components when operating at nominal load. The influence of the change in ambient temperature and block load on the value of exergy losses and exergy efficiency was taken into analysis. The analysis further includes the impact of steam block operation without high-pressure and low-pressure heaters on the exergy efficiency of the steam block. The goal of the analysis is to determine the functional state of individual steam block components after a long period of exploitation and maintenance in order to take appropriate measures to improve their technical performance. Exergy losses during nominal operation of the steam power plant unit are the largest in boiler and amount to 313.42 MW, followed by a turbine with 205.60 MW, condenser 1 with 6.03 MW, condenser 2 with 5.75 MW, while other components of the steam power plant have exergy losses in the range of 0.03 to 2.15 MW. Operation of the unit at nominal load without HPH results in an exergy efficiency decrease from 5.60 to 9.80 %, while in case of operation without HPH and LPH it results in a decrease in exergy efficiency from 9.86 to 16.40 % depending on the pattern used to calculate. The conclusion after the analysis indicates that the biggest exergy losses are in the boiler and turbine and consequently these components have the lowest exergy efficiency values. The increase in ambient temperature has different effects on individual components of the thermal power plant, increasing exergy losses of the boiler while reducing the turbine exergy losses and condensers.